Ureaplasma diversum was isolated or detected in four cases of Japanese Black and Japanese Polled aborted fetuses in Yamaguchi prefecture. In all cases pneumonic lesions were histopathologically confirmed. Pneumonic lesions had mononuclear cell infiltration mainly consisting of lymphocytes, which had characteristic nodules and follicles. In the placenta of Case 1 interstitial edema, mononuclear cell infiltration, arteritis with thrombus formation, and chorionic epithelial necrosis were present. In regard to the sensitivity of detection for U. diversum, the genetic test was more effective than cultivation. PCR using DNA that was extracted from paraffin-embedded lesion tissue showed a positive reaction regardless of the PCR negative when using organ emulsion. These results suggest the possibility that pneumonia and fetal placental lesions were caused by U. diversum and were related to the Japanese beef cattle abortions.
An imported sheep from New Zealand was diagnosed with Johne's disease from a real-time PCR test of fecal samples and was culled during the import quarantine. The DNA (0.03 to 0.163 pg/2.5 μl) of Mycobacterium avium subsp. paratuberculosis (MAP) was detected in the feces and a maximum of 14.3 pg/2.5 μl of DNA was detected in tissue specimens (ileum to cecum, mesenteric lymph nodes). In the bacterial cultivation of tissue samples using MGIT liquid medium, the growth of acid-fast bacteria was confirmed after 6 weeks of incubation and 200 to 600 pg/2.5 μl of MAP DNA was detected in the medium. DNA Typing by RFLP analysis of IS1311 PCR products revealed that the isolates were ovine-type but not bovine-type. The primary culture of the bacteria was only successful using MGIT liquid medium. However, a subculture of the isolate was possible from the liquid medium to Herrold's solid medium, and the colony growth was enhanced in the presence of 10％ CO2. Chronic granulomatous enteritis was observed histopathologically and such lesions were consistent with the isolated areas of the bacteria. Real-time PCR and bacterial isolation using MGIT liquid medium are highly sensitive and efficient diagnostic methods for detecting ovine Johne's disease.
Twin dogs, of a two month old intact female Labrador mix, had extensive congenital secondary cleft palates. Case 1 was operated on for a Sliding bipedicle flap that required re-suturing and reoperation due to partial cleavage. In Case 2, the Sliding bipedicle flap repair also used an autologous buccal mucosa graft and the symptoms disappeared with a good prognosis. In Case 1 where the Sliding bipedicle flap repair was performed, problems were caused by the instability of the bipedicle flaps and the excessive tension from the lack of the nasal mucosa as a result of the extensive defect. However, the autologous buccal mucosa graft was able to compensate for those disadvantages by reducing the tension on the nasal mucosa and stabilizing the bipedicle flaps in Case 2. The combination of the Sliding bipedicle flap repair and the autologous buccal mucosa graft may be effective for extensive congenital secondary cleft palates.
A chipmunk presented with marked swelling of the left testicle. Histologically, the testicular mass was composed of sheets of polyhedral cells consistent with a seminoma. Invasion of the neoplastic cells to the adjacent tissues and marked atypia were noted, such as atypical karyomegalic cells and multinucleated cells, as well as abnormal mitotic figures. The neoplasm was considered to be potentially malignant. To the best knowledge of the authors, this is the first report of seminoma in a chipmunk.