All issues
Volume 14, Issue 10
Displaying 1-9 of 9 articles from this issue
- |<
- <
- 1
- >
- >|
-
[in Japanese]1961 Volume 14 Issue 10 Pages 421-424,436
Published: October 20, 1961
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSDownload PDF (1171K) -
V. Evaluation and Application of Various Diagnostic MethodsI. OHISHI, S. KOBAYASHI, S. KUME1961 Volume 14 Issue 10 Pages 425-428
Published: October 20, 1961
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSComparison was made among three methods of diagnosis for canine filariasis:(1) the smear and stain method, (2) the concentration method, and (3) the intradermal reaction.
Experiments were performed with 103 dogs in spring and summer and 122 dogs in the other seasons. The dogs used were all more than one year old and autopsied at the end of the experiments.
Method 1 used a thick smear of 30cmm of blood collected from the auricular vein, which was stained with methylene blue. It gave a microfilaria detection rate of 93.5 per cent in summer and 75.5 per cent in winter when applied to dogs which were found to harbor microfilariae at autopsy.
Method 2 was applied to 1cc of venous blood, using aceton. A 100 per cent detection rate of microfilariae was obtained from this method, regardless of season.
Method 3 was carried out with antigen prepared from the body of adult canine filariae which had been fractionated with acetone after the treatment with sound-waves. The antigen was dissolved in physiologic salt solution at a concentration of 0.01 per cent and injected intradermally in the hypogastric region in a net amount of 10μg/0.1cc. Positive reaction was shown in 95 per cent of those which were found parasitized at autopsy and which, nevertheless, had been negative in the detection of microfilariae from the peripheral blood.View full abstractDownload PDF (1002K) -
K. MINO, G. OTSUKA1961 Volume 14 Issue 10 Pages 429-432
Published: October 20, 1961
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSof coagulase-positive staphylococci was devised by the authors. At first, a medium was prepared with the following composition: polypeptone, 10g; beef extract, 10g; sodium chloride, 75g; mannitol, 10g; lactose, 2g; and 0.2 per cent BTB solution, 12ml. It was adjusted to pH 7.2. Then it was distributed to test-tubes in 9-ml amounts and sterilized in the autoclave under a pressure of 15 pounds for 10 minutes. To each test tube, lml of 0.1 to 0.3 per cent solution of potassium tellurite in sterilized distilled water and then lml of the specimen to be tested were added. Cultivation was made at 37°C for 48 hours.
A loopful of the resulting culture was smeared on blood agar plate, Chapman's mannitol sodiumchloride medium, or staphylococcus medium No.110, so that coagulase-positive staphylococci might be isolated. This method was found to be practical.View full abstractDownload PDF (833K) -
I.Chick-Embryo Adaptation of Isolated Virus and Preparation of Dried Vaccine from Avianized VirusTSURUO ICHIHARA, TSUYOSHI ICHIHARA, S. WATANABE, Y. KITAHARA1961 Volume 14 Issue 10 Pages 433-436
Published: October 20, 1961
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSThe so-called mink strains of canine distemper virus isolated by the authors were subjected to serial passages through the chorioallantoic membranes of seven-day embryonating hen's eggs. As a result, two of the five strains used acquired adaptability to and lost pathogenicity for embryonating eggs when they had reached the 20th and the 35th passage, respectively. The resulting avianized live virus was inoculated into minks and dogs to confirm its safety. The high immunogenicity of this virus was proved in these animals which had been challenged with distemper virus or which had been exposed to contact with infected animals of the same species after vaccination with avianized virus. Therefore, it was made clear that this adapted virus was available to prepare very effective vaccine.
Dried vaccine was prepared experimentally from avianized virus. After storage at 4°C for 12 months, this vaccine still contained live virus, which was demonstrated by inoculation experiments on the chorioallantoic membrane. Satisfactory results were also obtained from mass inoculation of dogs in the field.
It was confirmed that the avianized live virus isolated by the authors was identical with the Lederle strain and other known chick-embryo-adapted live virus strains with regard to antigen when examined by cross immunization tests.View full abstractDownload PDF (746K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese]1961 Volume 14 Issue 10 Pages 437-438
Published: October 20, 1961
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSDownload PDF (348K) -
[in Japanese], [in Japanese]1961 Volume 14 Issue 10 Pages 439-441
Published: October 20, 1961
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSDownload PDF (532K) -
[in Japanese], [in Japanese]1961 Volume 14 Issue 10 Pages 442-445
Published: October 20, 1961
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSDownload PDF (7648K) -
[in Japanese]1961 Volume 14 Issue 10 Pages 446-450
Published: October 20, 1961
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSDownload PDF (1292K) -
[in Japanese]1961 Volume 14 Issue 10 Pages 451-454
Published: October 20, 1961
Released on J-STAGE: June 17, 2011
JOURNAL FREE ACCESSDownload PDF (1038K)
- |<
- <
- 1
- >
- >|