Twenty-nine cattle were examined. In them, 31 hoofs were affected with pododermatitis circumscripta, 4 with pododermatitis traumatica, and 2 with white zone disease. Technovit 6091 manufactured by Kulzer Co., Ltd., West Germany was applied to 15 hoofs of cattle showing remarkable supporting-leg lameness. The remaining hoofs served as untreated controls. The duration required for healing was 19.6 days in the experimental group with severe lameness and 21.5 days in the control group. A hoof affected with pododermatitis traumatica due to driving a nail and another hoof with white zone disease were complicated with severe deep sepsis. Within 1-2 days after the application of Technovit 6091, the state of burdening was markedly improved by simultaneous treatment with antibiotics every day in both cases.

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Hydroxyapatite (HA) has been used for treatment in periodontal and orthopedic surgery in human medicine. Granulated HA implant applied to two clinical cases. In one case a cat had a lesion in the frontal bone. In another case a dog had spondylolis thesis. Satisfactory results were obtained after granulated HA implant.

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Serum total cholesterol (T-CHO) were determined in a total of 715 lactating Holstein cows during a period from January to April, 1983. Parity, days after calving and milk yield influenced TCHO level significantly. TDN and CP levels in the ration, as well as body weight, were not significant factors. Morbidity was a factor for changes in T-CHO level. This level decreased significantly in cows with diseases. There was no marked difference in T-CHO level between cows with diseases and healthy ones.

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When a pregnant sow was inoculated subcutaneously with 10^6.0TCID₅₀/2ml of the HT^-SK-C strain at 74 days of pregnancy, it showed no clinical signs and the virus was not recovered from pharynx, larynx, nasal cavity or rectum. Viremia was not obscrvcd. At 104 days of pregnancy, it was sacrificedfor autopsy. An atempt was made to recover the virus from its fetus and their accessary organs. It had 13 fetuses, which were normal macroscopically. The inoculated virus was not recovered from the respiratory organs or rectum of 7 fetuses tested.
tested. Apregnant sow was inoculated subcutaneously with 10^6.0TCID₅₀/2ml of the HT-SK-C strain at 42 days of pregnancy. It was challenged intravenously with 10^5.0TCID₅₀/5ml of thc virulent strain at 66 days of pregnancy along with an uninoculated control pregnant sow. At 46 days after challenge (112 days of pregnancy), these pregnant sows were sacrified fbr autpsy The inoculated sow harborcd l5 fbtuses, which were all alive with no clinical signs. No challenge vrius was recovered from 8 fbtuses examined. The cohabiting control pregnant sow harbored l l fbtuses, of which 7 were hormal, 3 macerated and one was mummifbrm. The challenge virus was detected from tested organs. HI antibody titers of 5 gilts were 1: 40-1: 320 at 14 weeks after inoculation with 10^6.0TCID₅₀/2ml of the HT^--SK-C strain.

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Ferrous compounds were examined for effects on mice to reduce the toxicity of hexavalent chromium. When a chromate (as K₂Cr₂O₇) and FeCl₂ simultaneusly injected intraperitoneally (i.p.) to mice, there was a remarkable decrease in chromate-induced lethality. In mice administered orally or i.p. with both chromate and FeCl₂, the chromium content decreased in liver and kidneys, but increased in spleen and testis as compared with that in mice administered with the chromate alone. When injected i.p. with chromate and administered orally with FeCl₂, or pre-injected i.p. with FeCl₂ befor chromate injection, mice showed no remarkable alteration in the chromium content. FeSO₄ and chromate injected i.p. also exerted the same influence as FeCl₂. FeCl₃ and Fe (SO₄) ₃, had little influence on the chromium content. In mice simultaneously injected i.p. with chromate and FeCl₂, chromium excreted in urine was less than in mice administered with chromate alone.

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A high performance liquid chromatographic (HPLC) method was developed for the quantitative determination of guanidinosuccinic acid (GSA) and methylguanidine (MG) as uremic toxins in serum. The method involved protein precipitation with perchloric acid, and pre-column HPLC with benzoin. In it, 1.5M tris-phosphate buffer was used to stabilize the pH of the reaction mixture. Reaction was allowed to take place at 100°C for 4 min with the isocratic condition of HPLC. HPLC for simultaneous determination of GSA and MG was carried out on a Nucleosil 5C 18 column with methanol-0.5M tris-hydrochloric acid buffer (pH8.6)(65: 35) as the mobile phase. Recoveries of GSA and MG added to porcine serum to 5μg/ml were 93.7 and 90.1%, respectively. The limits of detection were 0.01μg/ml for each guanidine compound.

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When bovine colostrum was inoculated with 10³ and 10⁶/ml organisms of Salmonella typhimurium and S. dublin, there was no difference in the maximum bacterial count or survival period between the two species. When 7 colostral samples were inoculated with 10², 10³, and 10⁶/ml bacteria, one sample failed to show bacterial growth. In the other 6 samples the bacterial count reached 10⁷-10⁹/ml at 24-48 hours of incubation. It maintained this level for 1-5 days and then decreased. There was a variation of pH value of fermenting colostrum from 3.83 to 4.83 at which salmonellae perished. The survival period ranged from 3 to 20 days. There was no difference in it between the two species. In the colostrum in which fermentation was completed, salmonellae perished within 2 hours after inoculation.

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An improved method was developed from the conventional precipitation method for isolation of pathogenic bacteria from water.
Various kinds of bacteria grown in culture media were suspended in sterilized water. Ferric chloride and diatomaceous earth were added to the resulting suspension to a final concentration of 0.02 % and 0.1 %, respectively. The suspensions were adjusted to be neutral or slightly alkaline with 1 N sodium hydroxide solution. They were allowed to make precipitates for 90 minutes. Then the supernatants were removed. It was demonstrated that the precipitates held a majority of the bacteria. Furthermore, the bacteria could be recovered from the precipitate even if only a few bacteria (less than 100 cfu/liter) were suspended in the original water. This method is applicable to various kinds of water, such as drinking water, surface water, and drainage, if an adequate amount of diatomaceous earth is added to the water.

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A piglet 33 days old presented nervous symptoms on a farm in Mine city, Yamaguchi Prefecture, in May, 1985. Histopathological examination revealed purulent meningitis accompanied with purulent endophthalmitis and rhinitis. Streptococcus suis (Group T) was isolated from the brain, lung, eyeball, and nose. A combination of purulent endophthalmitis and rhinitis was regarded as a new form of streptococcal infection in pigs.

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A mesothelioma was found in a 4-year-old sow weighing 250 kg. Grayish white tumor masses 1 to 10 mm in diameter and round or flattened in shape were growing on the parietal and visceral pericardium. They showed reticular and tubular patterns lined with epithelioid cells. All the tumor cells contained hyaluronic acid.

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