A total of 44 ova with apparently normal morphology were collected nonsurgically from Holstein cows (I to VII-para) for basic breeding. They were transported to a place about 70 km distant and transplanted into the uterine horn on the same side with the ovary containing a corpus luteum in 44 nulliparous Holstein cows. These cows were selected in such manner that they showed estrus 24 hours or less before or after that in the donor cows. The transplantation was completed in 4-8 hours after collection when it was done by operation at the flank or by way of the cervical canal. As a result, 18 cows (40.9%) were judged to be pregnant by the rectal examination performed about 40 and 60 days after estrus noticed before the transplantation. It was anticipated that this technique might make it possible to apply the transplantation of fertilized ova to practical purposes.
Red-fin disease occurred continually in four schools of eels on a fish farm in Mie Prefecture for a year beginning with June, 1978. Affected eels swam inactively near the surface of water at 27-29°C, manifesting anorexia. Main autopsy findings were hyperemia and hemorrhage of the fins, abdominal region, and anus, congestion of the gills. and hemorrhage and discoloration of liver, spleen, kidney, and intestine. Aeromonas hydrophila was isolated from principal organs. The water of the fish pond was inferior in quality and transparency. The disease became under control by the improvement of environmental conditions and treatment with oxolinic acid.
Ducks of two groups, A and B, possessed HI antibody against Newcastle disease (ND) virus were studied. They were free from clinical signs and exhibited an HI titer of 1: 32 or less. An HI titer of 1: 4 or more was noticed in 49 of 105 ducks of group A and in 10 of 40 ducks of group B. Eleven strains of virus were isolated from 9 of 37 ducks of group A, and identified as those of ND virus, or a lentogenic virus. They agglutinated erythrocytes of chicken, duck, guinea pig, sheep, and cattle, but not those of horse. Their infectivity and hemagglutinability were completely inactivated by heating at 56°C for 60 and 30 minutes, respectively. No clinical signs were manifested by SPF ducks inoculated with the isolated virus at 3 or 80 days of age. The virus was recovered from these ducks 7 days after inoculation, when antibody was found to have been produced.
For the purpose of surveying the contamination and the gene-expression of murine leukemia viruses among experimental mice, the COMUL (complement fixation for murine leukemia) test was employed to detect specific antigen by the method reported by Huebner et al., USA. Antiserum was obtained from an immunized rabbit with the virus inner structural polypeptides antigens which had been produced from the virus treated with ether. The virus was produced from the persistent infected cell culture with murine leukemia virus, Friend strain. A purified and concentrated virus material was obtained by differential high-speed centrifugation in the centrifugal tube containing with gradient sucrose. Immune rabbit serum was employed to detect the main antigen of the viral polypeptides by the complement fixation reaction. The reaction was suggested a specific reaction mainly with p30 antigen by the immuno-gel-diffusion test, since one continuous precipitate line was observed among the serum prepared by the author and anti-p30 immune serum employed as reference, against ether extracted virus antigens. Rabbit immune serum was applied to the COMUL test for antigen detection with materials from the liver and the spleen prepared by ether extraction from mice collected in some laboratory. The result showed that the incidence of positive reaction was found low in the liver and high in the spleen. Contrary to the detection of antigen, the antibody detection was attempted by the immuno-gel-diffusion reaction with serums obtained from mice aged more than 6 months and was found negative in all the cases. Considering the results obtained together with the fact that mice diseased with leukemia a generally accompanied with splenomegaly, the test with rabbit immune serum will be useful for a preliminary survey of murine leukemia to detect specific antigen in spleen and tissue culture cells inoculated with test materials.
A diagnosis of aortic thromboembolism was made on two castrated Japanese indigenous male cats 4 years and 5 months and 6 years old, respectively. These cats was affected with cold sensation in both posterior extremities and pulsus deletus of the femoral artery. Aortography revealed hemokinetic disturbances around the point of origin of the external iliac artery. One dog died and confirmed to be affected with thrombosis at this point. Thombectomy was performed on the other dog, which died on the following day of the operation.