A new anthelmintic, tetrachlorodifluoroethane (TDE), was applied to the treatment of ruminant animals infected with Fasciola hepatica. 1. A dose of 100mg/kg of TDE was given to thirteen cattle (Japanese native oxen) naturally infected with liver flukes. Egg counts were carried out during some weeks before and after treatment. All cattle, but one, gave negative egg counts 28 days after treatment. 2. Seven cattle (Holstein cows) naturally infected were given a dose of 30 to 50mg. Three egg counts were made during four weeks after treatment. Counts were reduced in and after the second week. 3. Of six goats artificially infected, two were given a dose of 300mg/kg and the remaining four a dose of 500mg/kg. Autopsy was performed on each of them between 4 to 62 days after treatment. As a result, a dose of 300mg/kg showed such an effect as expected, killing about 80 to 100 per cent of the flukes parasitizing. The same result was obtained from the four goats given 500mg/kg each. 4. To eight sheep and twelve goats, both naturally infected, was administered a single dose of 500mg/kg of TDE in fleld trials. Egg counts were made on them 7, 14, 21, and 28 days after administration and gave negative results in 90 to 100 per cent of the animals tested each time. 5. Side effects, such as depression, a slight decrease in appetite, and diarrhea, were founded in a few animals 2 to 3 days after treatment.
A disease of unknown etiology with diarrhea as main clinical symptom broke out among swine in Kumamoto Prefecture in October, 1958, and in Fukuoka Prefecture in January, 1960. All adult pigs involved recovered, showing severe diarrhea for a day or two, but infected sucklings died without exception. A strain of virus was isolated and called the Ukiha. In cross-immunization tests, pigs conferred immunity against the Ukiha strain were protected completely from the infection with the Shizuoka and the New York strain of transmissible gastro enteritis (TGE) virus. Tissue emulsion was prepared from the intestnal wall of an infected pig and centrifuged. The resulting supernatant was inoculated in the amni otic cavity of embryonated eggs for serial passage of virus. Cultured tissue from diseased swine was administered per os to experimental pigs for serial passage. As a result, it was successful to infect pigs positively with the 10 th and 20 th egg-passage virus and the 10 th, 20 th, 50 th, and 70 th pig-pa ssage virus.