In this study, nasopharyngeal swabs (Swab) and bronchoalveolar lavage fluids (BALF) were collected from 50 Japanese black cattle diagnosed with severe chronic pneumonia, and identification of the causative bacteria of pneumonia and its antibiotic susceptibilities were investigated. Mycoplasma bovis and Pasteurella multocida were mainly isolated from Swab and BALF. However, the rates at which the same bacterial species were isolated from both specimens in the same cow were relatively low. In addition, M. bovis and P. multocida isolated from BALF were mainly sensitive to fluoroquinolones, but the antibiotic susceptibilities of the same two species isolated from Swab were low. From the above, M. bovis and P. multocida were mainly isolated from the nasopharyngeal area and bronchoalveolar area of cattle with severe chronic pneumonia, but the antibiotic susceptibility differs depending on the collection site, even if the same bacterial species is a possibility. It seems necessary to be careful about estimating the pneumonia causing bacteria by Swab in calves with severe pneumonia.
A female, 1-month-old Japanese Black calf, which had showed weakness since birth and had difficulty standing and walking, was clinically and pathologically examined. No abnormality was found in the hip joint with simple X-ray radiography. Macroscopic examination revealed a cavity with serous fluid at T2 and duplication of the spinal cord from T3 to the lumbosacral segment. Histological examination confirmed that the inner surface of the dilated central canal was lined with ependymal cells.
A case of acute erythremic myelosis (pure erythroleukemia) was detected in a Japanese Black cow with depression and emaciation. The white blood cell count was not very high, but there was a high percentage of neoplastic cells on blood smears. Necropsy examination showed marked enlargement of the spleen and some lymph nodes. The spleen, lymph nodes and thymus were almost completely replaced by neoplastic cells. The cells sometimes expressed hemoglobin (Hb), but the other lymphohematopoietic markers tested were absent. In contrast to a previously reported case with several mature erythroid cells, the current case was composed mostly of large blastic cells and was diagnosed as a blastic type of acute erythremic myelosis, which has not been reported in cattle to our knowledge. The diagnosis of erythroid neoplasms is usually based on Giemsa-stained smears of blood or bone marrow in veterinary literature. However, this report suggests that the diagnosis is more correct when Hb immunohistochemistry is applied to smears or formalin-fixed, paraffin-embedded tissue.