A three-year-old female Japanese indigenous dog displayed typical signs of hypothyroidism, including myxedema, low body temperature, low voltage on ECG and high level of serum cholesterol above 500mg/dl. The concentrations of serum thyroxine (T4) and TSH of the patient and control dogs were estimated by a commercial kit of radioimmunoassay. The level of serum T4was under 0.1μg/dlin the patient, and 0.98±0.5, ranging from 0.4 to 2.4, μg/dl in 10 control dogs. Serum TSH level ranged from 2.7 to 2.9μU/mlin the patient and from 0.9 to 2.1μU/ml, averaging 1.66 μ0.4μU/ml, in 5 control dogs. In the patient, serum levels of both cholesterol and creatine phosphokinase began to decrease after oral administration of thyroid extract, and serum T4level reached 0.4μg/dlon the 56th day after the treatment. In the patient and a control dog, the injection of 200μg of thyrotropin-releasing hormone (TRH) did not show apparent increase of their serum TSH level.
Induction of parturition was examined in fattening Holstein cows at various stages of pregnancy. Abortion was induced in most of cows administered with PGF2α at the early stage of pregnancy (1-4 months). Parturition was hardly accelerated in cows administered with PGF2α alone at the advanced stage of pregnancy (approximately5 months and later). Subsequent administration of estradiol with dexamethasone after the first PGF2α treatment caused abortion within 2-4 days. When it was not enough to cause abortion, further injection with dexamethasone, estrogens and PGF2α was required for abortion to occur in cows at the advanced stage of pregnancy. Parturition could always be induced artificially in cows at the advanced stage of pregnancy within 2-4days after the final treatment, although retained placenta was frequently observed in these cows.
Sensitivity and specificity for detecting antibodies against Brucella canis were compared between the rapid slide agglutination test (RSAT) and the tests conventionally usedin Japan (tube agglutination test, gel-immunodiffusion (GD) test) by using samples collected from field dogs, and experimentally infected dogs. Sera classified as negative by the RSAT were proved to have titers of 1: 80 or lower by the tube agglutination test. Sera classified as positive by the RSAT and 2-mercaptoethanol-treated RSAT (2ME-RSAT) had titers of 1: 160 or higher. All the sera with positive precipitation line in the GD test were included in the RSAT and 2ME-RSAT positive proup. The RSAT was evaluated as useful screening test for the serodiagnosis of Brucella infection, since it was simple and rapid with sensitivity and specificity comparable with those of the conventional tests, although B. canis organisms were detected earlier from the blood than by the RSAT. Of 368 serum samples collected at a dog pound in Hyogo Prefecture, 16 (4.3%) were classified as positive by the RSAT, 2ME-RSAT, and GD test with titers of 1: 160 or higher.
The first colostrum-fodingtime often birth, serum gamma-globlin, IgG, IgM and IgA levels in the serum and colostrum were determined in 73 Holstein-Friesian calves. Inter relation was observed among them, as well as the subsequent occurrence of clinical diarrhea in the first month of life. Significant positive correlations were observed between the colostrum-feeding time and the health of the animals; the serum gamma-globlin, IgG, IgM and IgA levels and the health of the animals; and the serum gamma-globlin, IgG and IgM levels and the colostrum-feeding time. The serum immunogloblin levels of the calves were influencedmore distinctly by the colostrumfeedingtime than the colostral immunogloblins levels. The calves had to be fed colostrum in the first hour often their birth to get high-level serum immunogloblin for defense against neonatal diarrhea
Fenchlorphos, an organophosphorus preparation, in a form of 1: 200 dilution of 25% enulsionwas used for dipping of 50 cats and 50 dogs from which Demodex canis, Notoedres cati, Otodectes cynotis, or Sarcoptes scabiei had been isolated. Four dippings at weekly intervals brought about complete healing. The preparation was also destructive to Ctenocephalidesc anis, C. felis, and Polyplax spinulosa
Seven neonatal piglets of a litter of ten were affected with gangrenous enteritis. They manifested no noticeable changes, but hemorrhage and necrosis of the small intestine. Clostridium perfringens type C was isolated from their organs. It was compared with C. perfringens isolated from feces of the dam of these piglets and the remaining 3 ones unaffected. There were no differences in biochemical properties between the two. When inoculated intradermally into rabbits, the organism of enteritic origin induced hemorrhagic changes, but that of fecal origin did not. All the strains of the former were sensitive to calbadox and kitasamycin, except two which were resistant to kitasamycin.