Carcases were artificially contaminated with Salmonella organisms, which were examined for rise and fall after that. When a piece of meat weighing 2g was dipped in a bacterial suspension at 5°C for 60 seconds, the bacteria contained in the piece began to decrease in number several days after contamination. Salmonella enteritidis and S. typhimurium, however, remained alive in the meat for 58 and 49 days, respectively. The surface of a thin piece of meat covering 2cm2 was painted with a bacterial suspension by the aid of a brush, and preserved at -2 to 2°C. In this piece, the bacteria began to decrease in number several days after painting. S. enteritidis and S. typhimurium, however, were alive even 103 and 80 days after painting, respectively.
A patho-epizootiologic survey on atrophic rhinitis (AR) in swine was carried out at the Sagamihara Slaughterhouse in order to reveal the rate of occurrence of the disease in Kanagawa Prefecture during 1968-1969. Of 41, 692 pigs inspected, 438 were clinically diagnosed as AR from a severe facial lesion and clinical signs. Of 184 pigs selected from a large number of pigs with no facial lesions, 52 were diagnosed as AR anatomically. These diseased animals showed atrophy of the nasal turbinates and facial bones, inflammation of the nasal mucosa, inflammatory exudation in the nasal cavity, and other clinical signs. Most of them remained of poor body weight, showing retarded growth.