Two each of fresh embryos collected by flushing after superovulation from 10 Japanese black cows were ipsilaterally and nonsurgically transferred into the uterus of 44 recipient Holstein cows. Twenty-six recipients (59%) became pregnant producing 10 (38%) twin calves, but abortion occurred in 2 (8%) cases. Two each of frozen embryos prepared by in vitro fertilization were transferred with the same manner into 402 recipient Holstein cows. Onehundred forty recipients (35%) became pregnant producing 17 (12%) twin calves, but abortion occurred in 14 (10%) cases. No significant difference was observed in the gestation period and the weight of calves between the two groups. Thirty-four calves were produced from the transfer of fresh embryos on 26 recipients and 143 calves were produced from transfer of frozen embryos on 140 recipients.
Two piglets aged 2 months in an integrally-operated swinery presented posterior trunk palsy and astasia. Autopsy revealed an abscess 3cm in diameter between the thoracic vertebral column and pleura, reaching the vertebral canal and compressing the medulla, which was curved with hemorrhage and neuronal degeneration and necrosis. Actinobacillus pleuropneumoniae (Ap) serotype 2 was isolated from the abscess. Neutrophil and lymphocyte infiltration was seen in some alveoli in the lungs. Ap serotype 2-specific antigen was detected inthe abscess and pulmonary lesions by enzyme-labeled antibody method. Antibody against Ap serotype 2 was detectable in not only the two diseased cases but also all other piglets reared in the same swinery.
Sixty-four Holstein calves, 24.5 day of age (mean), which were fed daily 4l of milk replacer containing 4% modified soybean protein, were examined for the incidence of diarrhea and protective effect of sodium cromoglicate (SC). Calves with significant increase in blood ammonia (BA) within 10 minutes after feeding (Group A, n=12) showed a higher incidence of diarrhea (58.3%), whereas the incidence was lower (12.5%) in those with decreased BA (Group B, n=16)(P<0.05). Of 36 calves with increase in BA after feeding, 23 calves, which were given 100mg/day SC with milk replacer, showed lower incidence (8.7%) of diarrhea as compared with 13 calves, which were given no SC showing high incidence (61.5%) of diarrhea (P<0.01).
The changes in amino acid composition in the horny hoof wall of dairy cows were investigated during the periparturition period, in relation with actual milk yield, dry matter intake (DMI), total digestible nutrients (TDN) and crude protein (CP). The growth of the horny hoof wall was most rapid during the dry period as well as one month prior to and one monthfollowing calving. The horny hoof wall during the dry period was revealed to have lower concentrations of glycine, methionine and histidine and high levels of proline. The concentrations of aspartic acid, threonine, alanine, valline, isoleucine, tyrosine and arginine were closely correlated to milk yield and to DMI, TDN and CP, while those of serine, glycine, phenylalanine and lysine were not to DMI, TDN and CP. From the contents of hystidine, lysine, and arginine the horny hoof wall was considered to be most hardly keratinized during the dry period, while most softened following calving.
Two cases of Yorkshire Terrier, showing clinically “honking” cough and dyspnea, and radiographically tracheal collapse were subjected to the partial excision of soft palate. Clinical signs were markedly improved after operation with decreased level of tracheal collapse by fluoroscopy.
bacteriological survey was performed with chicken meat from two retail shops, A and B, in Ebetsu city of Hokkaido during the period 1987 to 1992. The geometric means of standard plate counts (SPC) and coliform counts (CC) per gram samples from shop B were 7.1×106and 5.0×105 (N=61) in 1987, 2.0×106and 5.2×104 (N=10) in 1990, and 4.7×103and 1.6×102 (N=90) in 1992, respectively. The results were nearly the same with shop A. The isolation rates of Salmomella from shop B samples were 34%(N=61) in 1987, 40%(N=20) from March to August in 1990, 27%(N=52) from October 1990 to July 1991 and 8%(N=90) in 1992.