A complication of ovarian cyst and endometritis in a Holstein heifer, two years and ten months of age, was treated with hepatic stimulant and sexual hormone and held under long-term observation on morphological changes of the ovary and the state of appearance of estrus. Eventually it was slaughtered at the abattoir on account of serious prognosis. Histopathological examination was performed on its ovaries and revealed findings from which this case was diagnosed as stratumgranulosum cell tumor of a unilateral (left) ovary with follicle formation.
The streptococci isolated from various dairy products in 1955 and 1957 were studied and identified, being especially taken into consideration the enterococcus group. At the same time, some selective media for isolation, identification, and count of entrococcus-group organisms were examined. The results obtained are summarized as follows. 1) Of 99 strains originated from 76 materials, 78 were identified as Streptococcus faecalis and 3 as Str. liquefaciens, but 18 remained unknown. Str. faecalis was identified in 55 of 57 strains originated from powdered milk and only 26 of 42 strains from butter. 2) The thermoresistance of the enterococcus group cultured in skim milk was tested by heating at 65°C for 30 minutes and at 75°C for 15 minutes. Of 81 strains, 70 survived in the former test and 15 in the latter test. 3) The streptococci which showed typical growth both in B.T.B.azide dextrose broth and on B. T. B. azide dextrose agar modified by NASU were presumed to be of the enterococcus group. 4) The presence of ethyl violet in the broth was not necessary for detection of the enterococcus group in dairy products. 5) Luxuriant growth of the enterococcus group in the above-described broth and in 0.1% methylene blue milk generally occurred at 37°C rather than 45°C.
Surveys on monieziasis of sheep and goats were Conducted in several runs in the Kanto and the Northeastern regions of Japan. At first, various technics were examined for the efficiency of detecting cestode ova. The methods of examination used were as follows: detection of proglottid, filtration and floatation method with saturated saline solution, floatation method, filtration and precipitation method, filtration and precipitation method with formalin and ether, precipitation method, and direct smear method. The efficiencies of the last-named two methods were found to be too low for practical use.More over, vegetable fibers of fodder contained in the feces were apt to hinder the observation on para-site eggs. The efficiency of the directdeetection of proglottid was also extremely low.On the contrary, the filtration and floatation method with saturated saline solution was recognized to be the most effective in detecting moniezian eggs in the feces. Lambs and one-year-old sheep showed high infection rates. A survey on goat flocks revealed also a similar incidence level. In several runs, the rate of infection with moniezian cestodes among, sheep was as high as 30 to 80 per cent.