Anorexia, retarded growth, depression, leg weakness and blindness were observed in 13 day-old chicks of one layer flock. The mortality rate was 10%.Histopathologically, vacuolar changes were observed in the parenchyma of the optic lobe, medulla oblongata, spinal cord and retina. Ultrastructurally, vacuoles were found within and beneath the myelin sheath, made by the dissociation of myelin lamella. The same clinical signs and histopathological changes were reproduced in the chickens by feeding them the diet used in the flock. The diet contained lasalosid in addition to the premixed salinomycin. These clinical signs and pathological lesions suggest ionophore toxicosis as the cause of the disease.
Shiga toxigenicity examination was carried out on about 57 isolates of enterotoxaemic Escherichia coli from piglets with edema disease on nine farms. As a result, high pathogenic strains producing a large quantity of shiga toxins was detected from piglets on two farms. There were no correlations among the toxigenicity of isolates, serotypes and long-term edema disease outbreak farms.
To elucidate gaseous dynamics during abomasal displacement (AD), concentrations of H2 and CH4 in the exhaled breaths and the stomachs of cows with AD (n=20) were measured and compared with concentrations in cows without AD (n=10). Concentrations of H2 and CH4 in the rumen did not differ significantly between cows with and without AD, nor did they differ significantly between the rumen and abomasum of cows with AD. However, concentrations of H2 and CH4 in exhaled breath of cows with AD were significantly lower than those of cows without AD (5.2 ppm versus 22.1 ppm H2 and 694.8 ppm versus 6036.0 ppm CH4, respectively). Theseresults suggest that an excessive accumulation of abomasal gas, which is an essential prerequisite for AD, occurred due to the inhibition of eructation and inflow to the abomasum of gas produced in the rumen of cows with AD.
Fifty seven canine and 26 feline cases of malignant lymphoma were reviewed histopathologically and by immunohistochemistry and flow-cytometry using T-and B-cell markers. Based on these results, each case was diagnosed according to the WHO 2nd classification of malignantlymphoma of domestic animals. Anatomical classification was also conducted. In both animals, B-cell lymphomas were predominant in up to two-thirds of all cases. In particular, large B-cell lymphoma accounted for one-third of the cases. Of T/NK cell lymphomas, large granular lymphoproliferative disorders were most common in both dogs and cats. More than halfof canine lymphomas were multicentric forms, while feline lymphomas were observed in various organs.
Immunoblot analysis revealed canine heartworm (Dirofilaria immitis) infection in 19 cats (6.0%, 12 males and seven females) of among 315 domestic cats (136 males, 168 females and 11 unknown) in Yamaguchi Prefecture. No significant difference was observed based on the criteria of sex (P=0.095), with males (12/136, 8.8%) and females (7/168, 4.2%), behavior (P=0.594), with indoor only (5/90 cats, 5.6%) and both indoor and outdoor (11/199 cats, 5.5%), or age (P=0.559), with<2 years old (5/117 cats, 4.3%), 3-6 years old (6/86 cats, 7.0%) and 7<years old (7/92 cats, 7.6%). Nor was a marked difference observed among the eastern (7/106 cats, 6.6%), central (6/123 cats, 4.9%), western (5/65 cats, 7.7%) and northern (1/11 cats, 9.1%) parts of Yamaguchi Prefecture.
A 17-month-old male Welsh Corgi Pembroke presented with a three-week history of hematuria.An ultrasonographic examination of the urinary bladder revealed a marked thickening of thewall and remarkable unevenness of its mucosal surface. Treatment with antibiotics and hemostatics produced no clinical improvement. A partial resection of the bladder wall was performed for a histopathological examination one month after admission, and the lesion was diagnosed as embryonal rhabdomyosarcoma. The dog's condition gradually worsened and it died about two months after admission.
The authors investigated the tendency and feature of adverse reaction reports of rabies vaccines for animal use submitted from the 2003 to 2005 fiscal years. The results confirmed that the rabies vaccine was safer than commercial canine combination vaccines given the percentage of adverse reactions that appeared. However, adverse reactions occur frequently in dogs less than one year old and dogs between ten and twelve years old. Adverse reactionsalso tended to occur on the day of the vaccination, and particularly within six hours for serious cases. Moreover, this investigation showed that anaphylaxis accounts for half of all adverse reactions. The results suggest that dogs should be observed carefully on the day after the vaccination, as described in the insert.