A single dose of 33.50×53.28mg (as effective ingredient) of dichlorvos per kg of body weight was administered to 46 dogs. The rate of complete removal was 90.0% in 30 cases of ascariasis, 94.7% in 19 cases of hookworm infection, 74.1% in 27 cases of whipworm infection, and 0% in 22 cases of tapeworm infection. Such transient ill-effects as anorexia, depression, diarrhea of soft feces, and emesis were seen. No abortion or premautre delivery occurred to treated bitches. Cholinesterase activity began to decrease in value already an hour after medtcation, reaching a minimum at 12hours, and was 91.2% of a normal value at 120 hours. There was a tendency for erythrocyte count, hemoglobin amount, and erythrocyte volume to decrease and for the leukocyte count to increase. When administered with three times the abovementioned dose of dichlorvos, 2 dogs manifested toxic symptoms characteristic of poisoning of organic phosphorus, but survied. In conclusion, a single dose of dichlorvos was effective against the parasites studied, except the tapeworm, in the digestive tract of the dog.
A disease of unknown etiology attacked an animal in 1965, 6 animals in 1967, and an animal in 1968 at the “Y” Breeding Swine Center in Niigata Prefecture. It was characterized by sudden death preceded by few clinical symptoms. Autopsy on 3 cases of sudden death revealed nasal hemorrhage, jetting of stink from the transection of blood vessels and at the time of laparotomy, congestion and hemorrhage in the subcutaneous tissue and muscle of the neck, cardiac muscle, the gastric fundus, lymph nodes of the small intestine and other organs, hemorrhagic and edematous inflammation of the lung, and hemorrhage and partial phlegmonous changes by gas production of the liver. As a result, the disease was demonstrated to be enterotoxemia caused by infection with type-A organisms of Clostridium welchii.
Three dairy cows and 4 swine were submitted to autopsy within 1-6hours after found to be dead. No antemortem findings were known from them Autopsy revealed changes represented by spongy touch caused by foamy gas and bloody infiltration. A number of Gram-positive bacilli were observed in stamp smears of cardiac blood and pulmonary, hepatic, and muscular tissues. Type-A organisms of Clostridium welchii were principally detected from anaerobic cultures. Histopathologically, degenerative, hemorrhagic, and necrotic changes were seen in various tissues and organs. Mouse inoculation tests gave almost the same results.
1. Embryonated eggs were inoculated simultaneously with different doses of avian infectious bronchitis virus (IBV; the attenuated Nerima strain) and Newcastle disease virus (NDV; the B1 strain). In them, the propagation of one virus inoculated at the smaller dose was inhibited by the other virus inoculated at the larger dose. 2. No mutual interference was observed when 102.0-104.0 of IBV and 105.0 of NDV were incoulated simultaneously. Interference occurred when 105.0 of IBV and 105.0 of NDV were given simultaneously, and the IBV-neutralizing titer was lower than that of the control group. 3. IBV and NDV were administered at an interval of 3-10days. No interference took place when both viruses were given by nasal instillation even at an interval of 3days. Interference occurred between both viruses administered with drinking water at an interval of up to 7days. 4. Chicks bearing maternal antiby were inoculated simultaneously with both viruses. As a result, they showed only a lower IBV-neutralizing titer than those inoculated with IBV alone. 5. Chicks inoculated with IBV once at 28days of age showed a higher IBV-neutralizing titer at49days. of age than those inoculated with the same virus twice at 4 and 28days of age. Such tendency as this was not observed in NDV-neutralizing titer.
A Holstein grade female calf 17days old suffered from diarrhea occasionally and decreased gradually in appetite. It was sacrificed after 4weeks for autopsy. Main findings were diphtheroid enteritis in the jejunum, hemorrhagic erosions or ulcers in the abomasum, and petechial hemorrhage or necrotic focus in the liver, lungs, and mesenteric lymph nodes. Histopathological there was the formation of embolism in blood vessels containing a large number of hyphae. Furthermore, a grcat many hyphae invaded the edematous areas. these hyphae might have been spread over tous areas. There hyphae might have been spread over the whole body through the blood circulation. These results seemed to indicate that this calf had been involved in fungous infection.