A total of 52 cattle 3 to 10 years old naturally infected with liver flukes (Fasciola sp., ) were divided in to four groups. A control group consisted of 15 cattle. Groups A (8 cattle), B (13 cattle), and C (16 cattle) were administered orally with 3, 6, and 12mg/kg of triclabendazole, respectively. Group C maintained EPG at a zero level for 8 weeks. Biochemical analysis of the serum proved a decrease in γ-GTP and GLDH. These findings showed the fficacy of triclabendazole against liver flukes. Groups A and B also showed a decrease in EPG which failed, however, to reach a zore level. The dose of 3 to 6mg/kg was suggested to be insufficient. No side effects were recognized in any animal administered.
We induced renal dysfunction in dogs by giving them 3 mg/kg of mercuric chloride, and we then gave them electric acupuncture treatment in order to evaluate the efficacy of acupuncture and moxibustion therapy against systemic diseases in dogs. Symptoms improved in 4 of 6 dogs given electric acupuncture; the four animals showing improvement survived even after the end of the experimental period (10 days after the mercuric chloride was given). Increased GOT and BUN values in these four dogs reduced more rapidly than those in the control dogs. Marked cellular infiltration suggesting regeneration of necrosis was observed in the renal tissues. Symptoms such as anuria, vomiting or diarrhea did not improve in the 6 control dogs given no electric acupuncture; all of the control dogs died during experimental period. These result suggest that electric acupuncture restores renal function and repairs the renal tissues in experimental renal dysfunction; that the clinical application of electric acupuncture to dogs with renal diseases can be expected in the future; and that electric acupuncture is effective in the treatment of various systemic diseases.
Dogs were administered orally with 1 mg/kg of milbemycin D (MD). The blood concentration of MD reached a peak 1 hour after administration, but became undetectable (less than 0.5 ng/ml) 120 hours after administration. The number of microfilariae of Dirofilaria immitis (mf) in the peripheral blood decreased, showing a good correlation with the blood concentration of MD. It was reduced remarkably, but failed to be zero even 120 hours after administration. The following order of MD concentration was observed: blood plasma=brain<muscle<lung<kidney<lung<adipose tissue. This concentration was 1490.9 ng/g when it reached a peak in the adipose tissue. It ranged from 95.7 to 377.8 ng/g in the other tissues. It became undetectable (less than 5.0 ng/g) in the adipose tissue 240 hours after administration, showing that MD remained longer in this tissue than any other tissue, and that it had affinity to fat. MD concentration was 1.3 ng/ml at the highest in the cerebrospinal fluid.
The volume and nature of pus were examined in the uterine cavity of 33 bitches in which poymetra had been produced experimentally by injection of Escherichia coli into the uterus with the cervix ligated. The following results were obtained. The volume of pus varied from nearly none to 560 ml, or 56.89±107.31ml (mean±SD). It was more than 10 ml in 75.8% of the bitches. In the diestrous group it was 81.45±167.14 ml. There was a tendency that the volume of pus increased with the lapse of time. The pus was classified into three color groups, yellowish green, reddish brown and milky white. The yellowish green group was the largest and mainly seen from proestrus to diestrus. The reddish brown group was seen mostly in postpartum cases. The viscosity of pus varried from thick-souplike to pasty one. The thick-soup-like pus was found in 57.6% of the bitches. The trickling-threadlike pus was frequently seen in the diestrous cases.
An ldentification of 142 isolates of staphylococci from normal cow's milk was made by using kloos and Schleifer's simplified scheme. Its result was compared with that of identification by the Api staph system. Antibiotic susceptibilities of 138 of these isolates were carried out routinely by the agar dilution method with 10 antibiotics. The 142 isolates were identified as follows by the conventional tests: S. taphylococcus aureus, 17 (12.0%), S. simulans 49 (34.5%), S. xylosus, 30 (21.1%), S. saprophyticus, 13 (9.2%), S. cohnii, 12 (8.5%) and S. hominis, 10 (7.0%). The remaining 11 (7.7%) were not identified. The agreement of the result of identification by the Api staph systme was as follows: 100% for S. aureus, 89.8% for S. simulans and 60% for S. xylosus. As a whole, 55.6% of the isolates tested were identified correctly. All the isolates tested were susceptible to durgs, such as PC·G, AB·PC, CX, KM, CZ and S·T combination. Of the 138 isolates, 16 (11.6%), 8 (5.8%) and 1 (0.7%) were resistant to OTC and SM, CTC, and FM, respectively.
A total of 68 cases of bovine leukosis were found in Niigata prefecture from 1970 to 1985. Of them, 43 were studied. They consisted 31 of cases of the adult type. 7 cases of the thymic type. 3 cases of the calf type. one cese of the skin type and one case of the spleen type. The adult type developed tumor in the intrapelvic organs which used to be touched by rectal palpation. The thymic type showed swelling of the thymus and thoracic lymph nodes. The calf type developed symmetrical swelling of the superfidcial lymph nodes. The skin type showed remarkable tumors in the cutaneous tissue., The spleen type had no clinical characteristics. Pathologically, the adult type revealed massive and lumpy tumors in the serosa, loose connective tissue, urinary and genital organs, and their accompanying lymph nodes. In the thymic type, neoplastic proliferation was observed throughout such organs as the thymus, lung, liver, spleen, kidney, bone marrow and lymph nodes. The calf type had lesions similar to the those of the thymic type without tumor formation in the thymus. Neoplastic lesinons were observed in the cutaneous tissue of the skin type, and in the spleen and intrathoracic lymph nodes of the spleen type. All the cases of the spleen type and the adult type were proved to be BLV-antibody positive by the immunodiffusion test.
In December, 1985, abnormal and stillborn young were found in 26 neonates of 21 litters out of 37 neonates of 30 litters born from Suffolk ewes on 5 sheep farms in Aomori Prefecture. Six abnormal young showed almost the same symptoms, such as bending and crooking of the joints, including the fetlocks, of the four extremities, sigmoid rachiocampsis, hydrocephalus, glial nodule of the cerebrum, and degeneration and winding of skeletal muscle fibers. When determined, Akabane virus neutralizing antibody titer was 1: 64 in a neonate before having colostrum, 1: 16-1: 32 in 3 neonates after having colostrum, and 1: 8-1: 256 in 19 ewes raised in the same barn as the dams. It was concluded that the abnormal and stillborn young had been produced by infection with Akabane disease virus.
In November, 1985, 28 piglets (13 of group A and 15 of group B), approximately 60 days old, were purchased from two breeding farms and introduced into a fattening farm in Nagano Prefecture. After 2-3 days all the biglets of group A showed high fever and nervous symptoms, and 7 died within a few days. Two severely sick animals were examined. Haemophilus parasuis was purely isolated from main organs. Purultent fibrinous meningitis was observed in them thhistopathologically. The disease in question was diagnosed as H. parsuis infection associated with purulent brinous mentingitis.
Aortic body tumor was found in a 7-year-old Holstein cow with no clinical signs. A neoplastic mass was located at the base of the heart and covered with a fibrous capsule. It was 20×15×7 cm in size and brownish white in color. Metastatic lesions were observed in the premediastinal lymph node. Histologically, the tumor was composed of spindle-shaped and polyhedral cells. The tumor cells were subdivided into irregular lobules by connective tissue septa. Their cytoplasm was slightly eosinophilic and their nuclei were round to oval in shape. Electoron microscopy revealed that the tumor cells contained membrane-limited granules.