In August, 1966, blindness began to occur collectively among baby chicks caused by some commercial feeds in many districts of the western part of Japan. It usually affected some strains, such as Kimber K 44, Babcock B 390, and Warren Sex-Sal-Link F, but not a White Leghorn Strain.
The present experiment revealed that blindness had been provoked among chicks given feeds supplemented with ammeline and fish meal.
1. A specific substance causing blindness in baby chicks was an unknown organic compound contained in some fish meal. It had been added to the fish meal unlawfully as a false protein factor to increase the nitrogen content.
2. Regardless of the specific substance contained in the feed blindness was provoked in baby chicks by feeding a diet containing ammeline.
3. The clinical symptoms and gross and microscopic findings of the retina of blind chicks fed some fish meal were in accord with those of blind ones fed a diet supplemented with ammeline.
4. Blindness and dilatation of the pupil were observed in chicks 48hours to 7days after oral administration with ammeline added to a diet at more than 0.5%. Abnormal waves were found in the electroretinogram of chicks 24hours after administration with ammeline at a level of 3%.
6. Ammeline had a specific affinity to the retina of the chick. Early histological examination revealed the disappearance of fuscin granules from the retinal epithelium, conspicuous edema, degeneration and destruction of the visual cells, and retinal detachment. So that the chicks lost their own sight. Late examination revealed the lesions of the retina replaced partly by proliferated connective tissue fibers. The diminution and disappearance of paraboloid glycogen from the inner segment of the visual cells were demonstrated by using Bauer-Feulgen's method for glycogen.
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