Two male calves were inoculated subcutaneously with 10ml of blood infected with small-sized piroplasma and examined for clinical and hematological changes for 129 days after inoculation. As a result, there was an almost parallel relationship between the severity of anemia and the number of parasitizing protozoa, both of which reached a maximum level 50-70 days after infection. The anemia was accompanied by hyperchromic macrocythemia. The bone marrow began to show an enhanced function of producing cells of the erythrocytic series in the early stage of anemia. The enhanced function persisted in the convalescent stage after the erythrocyte count returned to normal. The ratio of myeloid to erylthroid cells reflected these changes well. Erythrocytes were reduced in resistance and serum lipoid phosphorus decreased in amount. These changes seemed to be related to the mechanism of hemolysis. A marked increase in serum gamma globulin was observed in the recovery stage of infection.
Four strains of acid-fast bacilli were isolated from a cow diagnosed as tuberculosis macroscopically and histologically at autopsy. In the primary isolation, small smooth colonies appeared about 20 days after cultivation and growth was stimulated by glycerol. Later, colonies were enlarged, but not pigmented. These isolates were weakly positive for catalase activity, but negative for niacin, nitrate reduction, urease, Tween 80 hydrolysis, and formamidase tests. Guinea pigs inoculated intraperitoneally with 0.2ml of 12-day-old culture in Dubos medium of these strains reacted more strongly to avian than mammalian PPD. At atuopsy, they has small lesions at the site of inoculation, in the peritoneal membrane, Liver, and spleen. The inoculated acid-fast bacilli were recovered from the lesions. No macroscopic lesions were observed in rabbits inoculated intravenously with 1.6×108bacilli, which were recovered from lung, spleen, and liver. There were no microscopic findings showing the presence of acid-fast bacilli. The isolates could be identified to beMycobacterium intracellulare.