A Japanese-Black beef calf presenting intermittent excretions of diarrhea and poor appetite. The animal food intake was insufficient, which resulted in delayed growth. The body weight was approximately 70 kg at 7 months old. Blood biochemistry examination revealed elevated levels of serum total bile acids (268 μmol/l) and blood ammonia (87 μg/dl), suggesting a congenital portosystemic shunt. Color Doppler ultrasonography provides evidence of this disease, such as turbulent flow between the portal vein and caudal vena cava outside the liver. During a laparotomy, contrast radiography of the portal vein did not reveal any contrast enhancement of the branched structures of the intrahepatic portal veins. Intraoperative portography revealed the apparent contrast enhancement of the branched structures of the intrahepatic portal veins, and the shunt was detected macroscopically by diagnostic interception using forceps. This was followed by the complete ligation of the Shunt vessel. The growth of the animal improved after the operation, and the animal's body weight increased to approximately 630 kg at 27 months old. In bovine practice, the use of portography during a laparotomy is helpful for diagnosing this disease and confirming the appropriate ligation region for a specific shunt vessel.