Serum α
1 acid glycoprotein (α
1AGP) was examined in cows with bovine leukemia (BL), and compared with other diseased cows. Inhibitory effects of α
1AGP isolated from a healthy cow and from a BL cow were also examined on the mitogenicity of healthy cow lymphocyte. In cows with BL and liver abscess, α
1AGP was detected as several protein bands, not observed in healthy cows, at the position of isoelectric point (3.2-4.2) by plate electrofocusing. By radioimmunodiffusion, serum α
1 AGP averaged 284.4±95.5 μg/m
l for healthy female cows (less than 450 μg/m
l in 95%) 316.7±101.3μg/m
l for reared cows and 395.7±295.5 μg/m
l for calves (those up to 14-day-old showed higher values compared with adults and reared cows). Cows with BL and other various diseases showed remarkably high α
1AGP levels, 1021.6±1747.6 and 1116.7±858.7 μg/m
l, respectively. In cows with BL, the following values showed positive correlation with serum α
1AGP concentration: WBC count, lymphocyte count, sialic acid value and LDH level. The inhibitory effects of each mitogen, which was added with refined cow α
1AGP and BL cow sera different α
1AGP content, were revealed on the blastogenesis of healthy cow lymphocytes almost proportionally to the α
1AGP concentration.
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