To examine the effect of prostaglandin (PG) F
2α and estradiol benzonate (E
2B) in inducing estrous signs in dairy cows without estrous signs, 15 mg PGF
2α and 4 mg E
2B were injected simultaneously into dairy cows with functional corpus luteum (CL) and acyclic or non-functional CL. Within 3 days after the injection, 79.1% of the cows showed estrous signs and were inseminated. The conception rate was 38.7% for those inseminations. Based on the plasma progesterone (P
4) level, the cows were defined as the functional CL group (P
4>1 ng/m
l) and the acyclic or non-functional CL group (P
4<1 ng/m
l). In the functional CL group, 100% of the cows were inseminated, and the conception rate was 35.3%. In contrast, 55.6% of the cows were inseminated and the conception rate was 30.0% in the acyclic or non-functional group. In the cows with functional CL and no estrous signs, the insemination rate was 79.2% and the conception rate was 39.5% when PGF
2α and E
2B were injected simultaneously. In contrast, the insemination rate was 60.4% and the conception rate was 37.9% when only PGF
2α was injected. The simultaneous injection with E
2B and PGF
2α brought about clear estrous signs and a higher insemination rate, although the conception rate was not improved. In conclusion, simultaneous injection with E
2B and PGF
2α may improve reproductive performance in cows showing no estrous signs.
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