Journal of the Japan Veterinary Medical Association
Online ISSN : 2186-0211
Print ISSN : 0446-6454
ISSN-L : 0446-6454
Volume 67, Issue 4
Displaying 1-6 of 6 articles from this issue
Farm Animal Medicine and Animal Health
  • Yoji FUKUI, Shozo ARAI, Shinichi SAKAKIBARA, Hiroshi SAWADA
    Article type: Original Article
    2014 Volume 67 Issue 4 Pages 249-254
    Published: April 20, 2014
    Released on J-STAGE: May 20, 2014
    JOURNAL FREE ACCESS
    Under controlled temperature and humidity conditions, we examined a method of measuring skin temperature in cattle using infrared thermography (IRT), and compared left-and-right differences in mean skin temperature. The coefficient of variation for maximum orbital temperature and mean body hindquarters showed good reproducibility, at 0.7% and 0.5%, respectively. Depending on the measurement distance, there were significant reductions in the maximum orbital temperature of cattle. In contrast, even if the measurement distance for IRT varied, the mean temperature of the body hindquarters was the same. No significant difference was seen between left and right skin temperatures in cattle body surface parts. In sites with exposed skin, such as the orbital area, the maximum temperature was automatically detected by IRT in the same place. However, in cattle sites with thick hair coverage, such as the hindquarter region, maximum and minimum temperatures were not detected by IRT in the same place. Therefore, in order to perform IRT measurements with sufficient reproducibility on areas in cattle that have a lot of hair, it is considered necessary to calculate the mean temperature of the area.
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  • Yoshio YAMAGUCHI, Takashi SIMODA, Kenichi MIYOSHI, Koumei SHIRASUNA, A ...
    Article type: Original Article
    2014 Volume 67 Issue 4 Pages 255-258
    Published: April 20, 2014
    Released on J-STAGE: May 20, 2014
    JOURNAL FREE ACCESS
    To examine the effect of prostaglandin (PG) F and estradiol benzonate (E2B) in inducing estrous signs in dairy cows without estrous signs, 15 mg PGF and 4 mg E2B were injected simultaneously into dairy cows with functional corpus luteum (CL) and acyclic or non-functional CL. Within 3 days after the injection, 79.1% of the cows showed estrous signs and were inseminated. The conception rate was 38.7% for those inseminations. Based on the plasma progesterone (P4) level, the cows were defined as the functional CL group (P4>1 ng/ml) and the acyclic or non-functional CL group (P4<1 ng/ml). In the functional CL group, 100% of the cows were inseminated, and the conception rate was 35.3%. In contrast, 55.6% of the cows were inseminated and the conception rate was 30.0% in the acyclic or non-functional group. In the cows with functional CL and no estrous signs, the insemination rate was 79.2% and the conception rate was 39.5% when PGF and E2B were injected simultaneously. In contrast, the insemination rate was 60.4% and the conception rate was 37.9% when only PGF was injected. The simultaneous injection with E2B and PGF brought about clear estrous signs and a higher insemination rate, although the conception rate was not improved. In conclusion, simultaneous injection with E2B and PGF may improve reproductive performance in cows showing no estrous signs.
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  • Hirotake KIMOTO, Ryoutarou NAKASHIMA, Masakiyo OZONO, Taketoshi SAITOU
    Article type: Short Communication
    2014 Volume 67 Issue 4 Pages 259-262
    Published: April 20, 2014
    Released on J-STAGE: May 20, 2014
    JOURNAL FREE ACCESS
    Trichuriasis was found in piglets at a simple nursery with a paddock on a commercial farrow-to-finish swine farm in Kagoshima Prefecture. There had been a history of trichuriasis on this farm four years ago, when sawdust was used as compost. This nursery was built on a field where the sawdust had been spread. The piglets were being grazed on the paddock for the first time. The EPG of the Trichuris suis eggs was measured in soil samples using the O-ring method. Trichuris suis eggs were recovered from the pig house nursery (600 EPG), two plots on the paddock surface (680 and 800 EPG) and the remaining sawdust from four years ago (160 EPG). Embryonated Trichuris suis eggs were observed in all soil from which eggs were recovered. These observations suggest that this case was caused by the embryonated eggs from four years ago remaining in the soil that had been spread with untreated compost. This report demonstrates the importance of examining the soil of any land piglets have been grazed on for Trichuris suis eggs.
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Small Animal Medicine
  • Hiroki YAMAZAKI, Satoshi TAKAGI, Kenji HOSOYA, Yuki HOSHINO, Masahiro ...
    Article type: Original Article
    2014 Volume 67 Issue 4 Pages 263-268
    Published: April 20, 2014
    Released on J-STAGE: May 20, 2014
    JOURNAL FREE ACCESS
    Canine histiocytic sarcoma (HS) has aggressive local infiltration and high metastatic rates. The morphology of this tumor shows highly undifferentiated cells with a mixed pattern of round-cell and mesenchymal appearances. These features can therefore sometimes be misleading and cause difficulties in achieving a definitive diagnosis. Immunohistochemical staining using canine antibodies targeting CD11d, CD18 and MHC class II is helpful for the final diagnosis of canine HS, however this requires special techniques and equipment. We had reported previously that the mRNA expression levels of the four surface antigens CD11b, CD11c, CD86, and MHC class IIα between canine HS and other disease samples were comparatively analyzed using qRT-PCR, resulting in the establishment of diagnostic tools for canine HS. The aim of this study was to evaluate diagnostic accuracy for suspected HS dogs clinically using this genetic diagnostic tool. Twelve dogs with suspected HS were included. First, the mRNA expression of each surface antigen in these samples was analyzed, and we applied the expression levels to the diagnostic criteria for canine HS. From the results, eight of the twelve dogs were estimated to have HS using this method. Finally, the estimated eight dogs were histologically determined to have HS, and the remaining four dogs were excluded from having HS. These results indicated that cell surface antigen analysis using qRT-PCR has the potential for clinical application, and may be useful as an adjunctive diagnostic tool for canine HS.
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  • Hitoshi ANO, Miho FUJINO, Hiromu KATAMOTO
    Article type: Short Communication
    2014 Volume 67 Issue 4 Pages 269-273
    Published: April 20, 2014
    Released on J-STAGE: May 20, 2014
    JOURNAL FREE ACCESS
    A thirteen-month-old castrated female Somali cat was presented for the investigation of petechiae of the skin's surface and thrombocytopenia. Based on the hematological examination, bone marrow cytology and differential diagnosis, the cat was diagnosed with idiopathic immune-mediated thrombocytopenia and treated with prednisolone and cyclosporine. However, the response to the treatment was poor. Furthermore, intravenous human immunoglobulin administration showed only a temporary effect. We therefore performed a splenectomy. Afterwards, the platelet count recovered once, but it subsequently decreased recurred following a reduction in the prednisolone dose. We therefore performed combination therapy of prednisolone and cyclophosphamide. The platelet count recovered promptly, and a good response was obtained thereafter. In this case, after attempts were made to investigate the therapeutic effects of various drugs on the feline immune-mediated thrombocytopenia, it was suggested that a combination of a splenectomy and drug therapy might be effective even when the reaction to the drug therapy was poor.
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Veterinary Public Health, Wildlife and Environmental Conservation
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