巻号一覧
14 巻, 2 号
選択された号の論文の3件中1～3を表示しています
- |<
- <
- 1
- >
- >|
-
基礎的検討およびその血清インスリン抽出への応用林 正紀, 赤沼 安夫, 葛谷 健, 井出 健彦, 葛谷 信貞1971 年 14 巻 2 号 p. 63-70
発行日: 1971/03/31
公開日: 2011/08/10
ジャーナル フリーSepharose coupled with insulinantibody was used for the extraction of insulin from serum samples. The principle of this method is based on the absorption of insulin to Sepharose particles which were attached by its specific antibody to make advantage for the separation of antigen-antibody complex from the solution.
A globulin fraction of guinea pig anti-insulin serum (AIG) prepared by salt precipitation was allowed to react with Sepharose 2 B which had been activated with CNBr according to Axen-Porath's method. One g wet Sepharose bound 5.4 mg to 10 mg AIG protein. AIGSepharose complex adsorbed 134 insulin at pH 8.2 while its binding to control Sepharoses (CNBr-activated Sepharose, Anti-HGH globulin-Sepharose and Normal guinea pig globulin-Sepharose) was almost negligible. On the other hand 125I-HGH was bound to Anti-HGH globulin- Sepharose whereas its absorption to AIG-Sepharose was minimal. Thus was the specificity of antigen-antibody reaction preserved even after the coupling of antibody to Sepharose. The maximal binding capacity of AIGSepharose to pork insulin was about 420-460 mU per g of the complex.
Most of the bound insulin was dissociated by the addition of 1 M acetic acid or 0.01 N HC1 to AIGSepharose. The matrix restored its insulin binding capacity by washing with Tris-HCl-albumin buffer (pH 8, 2)
290 ml of solution of pork insulin (109 μU/ml) was applied to the AIG-Sepharose column to fix insulin to the matrix, and then 1 M acetic acid was passed through this column to dissociate the bound insulin. The insulin concentration of effluent reached the maximum of 2, 980μU/ml during the dissociation.
The application of this complex for the extraction of insulin in serum was studied with the use of dog pancreatic vein serum samples containing insulin from 740 to 1, 575 μ/ml as measured by immunoassay. The recovery of serum IRI was in the range of 80 to 90 96.
Our present study suggests that the anti-insulin globulin-coupled Sepharose is applicable for the extraction of insulin from the various biological fluids. The method using antibody-Sepharose column seems also useful for the separation of various peptide hormones or macromolecules, whenever their antibodies are available.抄録全体を表示PDF形式でダウンロード (1622K) -
とくに代謝に及ぼす影響について吉村 望, 吉武 潤一1971 年 14 巻 2 号 p. 71-78
発行日: 1971/03/31
公開日: 2011/08/10
ジャーナル フリーThe effects of the intravenous infusion of dihydroxyacetone (DHA) on the metabolism and insulin release were investigated in nondiabetic and diabetic human subjects and in some patients having endocrine abnormalities.
1) In non-diabetic subjects, metabolites changes suchas decreases in the levels of blood sugar, plasma inorganic phosphate, free fatty acids and total acetone bodies were observed significantly, while the blood lactate concentration increased in parallel with the pyruvate and plasma immunoreactive insulin (IRI) levels tended to rise.
These findings suggest that administered DHA seems to be utilized via glycolytic pathway.
2) In well controlled diabetics, very similar effects seen in non-diabetics on the metabolism were observed. However, there were an increasing tendency in blood sugar and plasma glycerol and a profound drop in blood redox potential in diabetics not satisfactorilly controlled.
Therefore in not well controlled diabetics, these results ndicate the possibility that the administered DHA might be metabolized mainly via the gluconegenic pathway rather than glycolytic.
3) DHA showed the hypoglycemic effects on the patients of acromegaly or primary aldosteronism in some extent, and especially in case of insulinoma.
4) It was ascertained that DHA had an insulinogenic action even in diabetics as well as in those with acromegaly and aldosteronism.抄録全体を表示PDF形式でダウンロード (998K) -
1971 年 14 巻 2 号 p. 79-136
発行日: 1971/03/31
公開日: 2011/08/10
ジャーナル フリーPDF形式でダウンロード (8255K)
- |<
- <
- 1
- >
- >|