Patients with diabetes prick their skin for blood glucose monitoring.They subsequently insert a needle for injecting insulin. Because two needle sticks are necessary to complete these maneuvers, a major stress is potentially present for patients anticipating two painful procedures in succession. To slove this problem, we suggest a new system, in which a small amount of blood can be vacuumed from the needle insertion site of insulin injection and used for blood glucose measurement. In this paper, we would like to explain our clinical results in humans. Eleven healthy men and 23 diabetic patients were studied by comparing blood glucose data obtained by the conventional method and the new method. As a result, the blood glucose level obtaned by the conventional method was 165±73mg/dl (mean±SD), and that by the new method was 164±73mg/dl. The correlation was strong (0.99;p<0.001). During the procedure used for the new method, no subjects complained of any pain on vacuuming. This indicates that the two maneuvers can be safely accomplished from the same stick.Thus, patients will be able to share in the benefits of the new system, which makes blood glucose measurement maneuvers completely free of additional pain.