Anti-tumor activity of the human blood monocytes against cultured human cancer cells was examined in vitro. The cell lines used in this study were Okajima (a stomach cancer), KatoIII (a stomach cancer) and Hattori (a breast cancer). The anti-tumor activity of the monocytes from healty volunteers was not detected by 51Crrelease assay of the target tumor cells, but was demonstrated by 3H-thymidine incorporation test. This suggested that the anti-tumor activity was attributed to the cytostatic action. The inhibition of the growth of the tumor cells was observed irrespective of their origin. The growth of human normal fibroblasts was not inhibited by the activity of the monocytes. The anti-tumor activity was enhanced by in vitro treatment of monocytes with immunopotentiaters, such as Propionibacterium acnes (P. acnes), P. acnes-cell wall skeleton (CWS), a streptococcal preparation (OK-432) and Nocardia-rubra-CWS. The activity was not enhanced by protein bound polysaccharide Kureha (PSK). The activity of the monocytes from human umbilical cord blood was almost same to that of peripheral blood monocytes from healthy volunteers. The antitumor activity of peripheral blood monocytes from patients with head and neck cancer was also almost same to that of monocytes from healthy volunteers and was enhanced by the treatment with those immunopotentiaters in vitro.
The authors asserted the importance of auditory screening test for school children not only in the medical, but also in the educational point of view and presented a new method of screening test. Sixty five thousand seven hundred and eighty nine primary and middle school children were tested by this method in 1979. The method are as follows: When a child fails to respond to 20dB (JIS) pure tone in 1kHz or 4kHz, he is tested immediately at 40dB level. Thus one can obtain more information as to the nature and grade of hearing impairment of the child than the usual screening test. The authors classified the hearing status into No. 0 (normal) to No. 8 types. According to this classification the hearing of both ears of the child is described in 2 numbers for example 2-5. This method may be very useful for statistical purpose as well as for individual care of the hearing impaired school children.
A 35-year-old male patient visited our clinic complaining of a mass in his right parotid region for the past three months. The mass increased in size slowly and was 3×3cm in diameter. It was elastic and hard in consistency, and free from adhesion to the skin and surrounding tissues. Facial nerve palsy was absent and no lymphadenopathy was noted. The tumor was removed with the diagnosis of Warthin's tumor. It was located in an intraglandular lymph node of the parotid gland and was histologically diagnosed as nodular lymphoma of small cleaved type. He was treated with irradiation of 5000 rads of cobalt-60 postoperatively to the bilateral parotid and whole neck regions. The literature on malignant lymphoma of the parotid gland was reviewed.
Phonodynamic studies were carried out on live canine larynges with normal or pathological vocal folds. Experimental phonation was achieved by air flow blown through a cut-end of the trachea. Prior to the experiment, bilateral vocal folds were sutured at the cartilaginous portion. In addition, bilateral recurrent laryngeal nerves were prepared for stimulation to drive the thyroarytenoid muscles after severance of the PCA branches. Experimental data obtained were as follows; 1) subglottic pressure, 2) sound intensity, 3) air flow rate, 4) fundamental frequency, and 5) vibratory pattern photographed by an ultra high-speed camera. From results obtained, we concluded as follows; bilateral stimulation of the RLNs resulted in increase in intensity of the sound and subglottic pressure ane decrease in fundamental frequency of the sound. In addition, a better glottic closure with quasi-regular vibratory pattern was obtained by bilateral RLNs stimulation. These facts indicated that better vocal efficiency was obtained. These results were discussed mainly from the view point of phonodynamics and their implication for phonosurgery.
A case of EBNA-positive non-Burkitt's lymphoma of the nasopharynx is reported. The patients was 19-year-old female who complained of headache and bilateral cervical lymphoadenopathy for the past one month. Otolaryngological examination revealed a tumor on the lateral and superior wall of the nasopharynx. The tumor was histologically diagnosed as non-Hodgikin's lymphoma (non-Burkitt's type). Serologically, viral capsid antigen and early antigen to Epstein-Barr virus were 640-fold and 40-fold, respectively. EBNA was also detected in the biopsy specimen by anti-complement immunfluorescent test. The patient was treated by irradiation with a dose of 4, 000 rads to the nasopharynx. Also, 4, 000 rads were given to the whole neck, mediastinum and bilateral axillary region. Combination chemotherapy using bleomycin, vincristine, cyclophosphamide and adriamycin was also concurrently performed. She is doing well 3 months after the treatment.
The resonance frequency, Q-value of resonance and damping ratio of the vocal fold were estimated in living humans using the ultrasonic pulse method. In the present study, the transducer was placed on the thyroid lamina so that the ultrasonic beam would irradiate the margin of a vocal fold perpendicularly. A vibrator is placed on the skin over the midline of the thyroid cartilage to provoke an oscillation with the frequencies ranging from 30 to 300Hz continuously. The resonance pattern of vocal fold was recorded in the quiet inspiratory phase or in the phase of phonation neutral. The results obtained were as follows: 1) The resonance frequency of the thyroid cartilage was found in the range between 70 and 80Hz, and the Q-value between 2 and 3, while the resonance frequency of the vocal fold was found in the range of 110-120Hz with the Q-value of 4-6. 2) Little difference was observed regardless of the site of the vibrator and the transducer. The resonance frequency of the vocal fold was usually found in the range of 100-110Hz during quiet respiration. 3) Resonance frequencies and Q-values in the excised larynges were approximately 100Hz and 2.2-2.9, respectively. 4) In male adults, resonance frequencies of the vocal fold ranged from 91 to 145Hz(average 128Hz), and in female adults, 115-167Hz (average 136Hz), while Q-values ranged from 2.1 to 5.1 (average 3.4) in male, and 2.6-7.1 (average 4.0) in female. 5) In a male vocal fold, during phonation neutral at low pitch, only one resonance occurred at a frequency value close to the phonation pitch. However, two resonance points were usually observed in a female vocal fold and also in a male vocal fold at high pitch. The second resonance frequency was almost equal to the phonation pitch. Regarding the Q-value, no significant differences were found between the two resonance points. at a frequency value close to the phonation pitch. However, two resonance points were usually observed in a female vocal fold and also in a male vocal fold at high pitch. The second resonance frequency was almost equal to the phonation pitch. Regarding the Q-value, no significant differences were found between the two resonance points.
The lipid metabolism in the guinea pig cochlea was studied by means of autoradiography with 3H-glycerol. Following results were obtained. 1) In this autoradiographic study, the most heavily labelled cells were the outer hair cells, especially intensive along the cell membrane, where there were the sheets of endoplasmic reticulum (subsurface cisterna). 2) In the hair cells the radioactivity increased gradually as the apex was approached, but in the other cells this longitudinal gradient was not seen. 3) Kanamycin depressed the incorporation of 3H-glycerol into the hair cells, especially outer hair cells. These results, together with the results obtained by biochemical and histochemical studies (ref. 2nd report), will be discussed in 2nd report.
The lipid metabolism in the guinea pig cochlea was studied biochemically and histochemically. The results from this study, together with the results obtained by autoradiographic study (ref. 1st report), were discussed. Following conclusions were obtained. 1) Autoradiographic study by perilymphatic perfusion of the guinea pig cochlea with 3H-glycerol proved to be fit for the purpose of investigation on the localization of the phospho-lipid metabolism activity in the cochlea. In the autoradiographic study, the most heavily labelled celles were the outer hair cells, especially intensive along the cell membrane, where there were the sheets of endoplastic reticulum (subsurface cisterna). It would appear that subsurface cisterna in the outer hair cells synthesize phospholipids and then supply other biomembranes with these phospholipids. 2) Kanamycin depressed the incorporation of 3H-glycerol into the membranous cochlea. Auto-radiographic study demonstrated that this depression was due to the decreased incorporation of 3H-glycerol into the hair cells. 3) The turnover of phosphatidyl choline was the most rapid and the rate of decrease in this lipid content by kanamycin was the highest among the all lipid fractions. It seems that inhibition of phosphatidyl choline metabolism as well as that of polyphosphoinositides by aminoglycosidic antibiotics is closely related to the mechanism of cell injury by these drugs. 4) Histochemical and autoradiographic studies showed that the lipid globules in Hensen's cells contained hydrophilic lipids. These data, together with other data, suggest that the globules may function as an energy source as well as mechanical support of the organ of Corti. It is desirable that the prospective advances of the research on the lipid metabolism in the cochlea will contribute to discovery of a preventive and a treatment for sensori-neural hearing loss. The present study suggests that CDP-choline is useful for a preventive or a treatment.