The present work reports the results of radioallergosorbent test (RAST) with nine different grasses in 70 cases of grass pollinosis. The data are expressed as mean RAST score and percentage of positive RAST: Poa pratensis (3.30, 97.1%), Dactylis glomerata (3.16, 97.1%), Anthoxanthum odoratum (3.06, 94.3%), Agrostis alba (3.04, 92.9%), Lolium perenne (3.03, 94.3%), Phleum pratense (2.96, 91.4%), Festuca elatior (2.94, 90.0%), Phragmites communis (1.77, 64.3%) and Cynodon dactylon (1.71, 62.9%). These results lead to the following conclusions: 1) In the cross-allergenicity, both Phragmites communis (common reed) and Cynodon dactylon (Bermuda grass) are different from the other grasses. 2) For diagnostic screening purposes, it is sufficient to use the RAST with Poa pratensis (June grass) or Dactylis glomerata (Orchard grass).
Ultrastructural localization of K+ -paranitrophenyl phosphatase (K+ -NPPase) activity of the Na+, K+ -ATPase complex, nonspecific alkaline phosphatase (ALPase) and Mg2+ -ATPase activities were studied in the rat and human salivary glands. The results were summarized as follows. 1) K+ -NPPase activity: In the acinar cells and the striated ducts the reaction products were present on the cytoplasmic side of the plasma membrane forming basal infoldings, while no reaction was localized to the apical membrane. When ouabain was added to the medium, the reaction was completely absent. 2) AL Pase and Mg2+ -ATPase activities: Myoepithelial cells were demonstrated by an ALPase technique in rat, but not in human adult, and by a Mg2+ -ATPase technique in rat and human adult. Ultrastructually, the reaction products were associated with the plasma membranes and pinocytotic vesicles of the myoepithelial cells. The salivary glands of the 16 week-old fetus were composed of the primary ducts and the terminal cell clusters. Some flattened cells with bundles of microfilaments were present on the periphery of the terminal cell clusters. The Mg2+ -ATPase reaction products were found along all adjoining cell membranes, while no AL Pase reaction was observed.
The round window has become a subject of interest recently as a route to treat inner ear diseases. A study of the anatomy of the round window niche, round .window niche membrane and round window membrane was performed in 205 temporal bones. 1. The round window niche was examined under an operating microscope to determine the presence or absence of the round window niche membrane. Of 100 adult temporal bones, 30 had clear round window niches without round window niche membrane (open type), and 70 had round window niche membranes. The round window niche membranes were classified into three types. The closed type round window niche membrane covers the round window niche without any perforation. The perforated type had one or more perforations and the reticulated type had a lace-like membrane. 2. The measurement of the round window niche and round window membrane was performed by LUZEX 500. The round window niche (32 ears): area 2.85±0.78mm2, maximum length 2.30±0.37mm, width 1.77±0.28mm, superior wall 1.76±0.28mm, anterior wall 1.95±0.34mm, posterior wall 2.08±0.33mm. The round window membrane (45 ears): area 3.56±0.91mm2, maximum length 2.50±0.06mm, width 1.95±0.28mm. 3. The round window membrane consists of 3 layers: the outer layer, middle layer and inner layer. The middle layer occupies the largest part of the membrane (thickness 50-70μm). Collagen fibers in the middle layer run from the crista semilunaris to the margin of the round window like a fan. The round window niche membrane also consists of three layers: epithelial layers and subepithelial connective tissue layer. The latter loosely contains collagen fibers, fibroblasts and capillaries. Thickness of the niche membrane is 15-150μm. 4. The round window membrane is mathematically expressed and is displayed as a computergraphic.