This paper deals with two-year successive culture of human cancerous cells derived from squamous cell carcinoma of the maxillary sinus. The culture of the cells described in this paper has continued since June 4, 1975, and this cell line is considered to be stable and established. With a view to identifying the cultured cells, the author has been analyzing the cytologic findings by the seven major research tools which are detailed later.
The patient was a 48-year-old man with well differentiated squamous cell carcinoma. In the maxillary sinus classified to T3N1M0. This patient underwent necrotomy twice, but died in October, 1976.
1) In photomicroscopic study, most cultured cells were of sheetlike epithelial type which grew in multilayers and the superficial layer was cornified.
2) Electron microscopic study showed that the cells possessed desmosomes and tonofilamentsand were of eqidermoid origin.
3) Chromosomal studies showed that the distribution of the number at the 5th and 25th generations fell in the hyperdiploid range. Karyotype analysis showed the presence of several chromosomes suffering constitutional aberration. These chromosomes served as the marker.
4) The plating efficiency was about 42% in a modification of Eagle's MEM, and about 20% even in 0.3% soft agar suspension.
5) The growth curve showed that a period of 6 days was required for the cells to attain a 4-to 5-fold count increase.
6) Five of six nude mice given the cultured cells successfully developed tumor. The tissue of this tumor was characterized by relatively clear cytoplasm and anisonucleotic nuclei, and proved to be identical with the cells that had been groan by the patient, on accountof the high nucleocytoplasmic ratio.
7) The dispersed cells of the successively cultured tumor were found to agglutinate even in low concanavalin A concentrations.
These findings convinced the author that the successively cultured cells are derived from the epidermoid cells of human malignant tumor.
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