日本耳鼻咽喉科学会会報
Online ISSN : 1883-0854
Print ISSN : 0030-6622
ISSN-L : 0030-6622
88 巻, 4 号
選択された号の論文の10件中1~10を表示しています
  • 岡本 牧人, 高橋 広臣, 八尾 和雄, 藤野 明人, 石井 豊太, 原田 宏一, 屋宜 晃, 吉尾 知, 八木 一記, 古沢 慎一, 籾山 ...
    1985 年 88 巻 4 号 p. 443-448
    発行日: 1985/04/20
    公開日: 2008/03/19
    ジャーナル フリー
    Clinical utilities of the measurement of the subsets of the lymphocyte in peripheral blood of the 47 head and neck cancer patients were investigated.
    Lesion of the subjects were larynx (21), paranasal sinus (8), epipharynx (4), mesopharynx (4), tongue (4), parotis (3), cervical esophagus (2) and ear (1). Histologies of them were squamous cell carcinoma (38), adenocarcinoma (5), malignant melanoma (3) and malignant lymphoma (1).
    Lymphocytes of the patients were incubated with monoclonal antibody marked with fluorecent substances, then were counted by fiuorocytometer EPICS V(C).
    Most useful parameter was the numbers of OKT4+ lymphocytes, because they were well correlated with clinical course and prognosis.
    Although percentage of OKT8+ lymphocytes was elevated in the condition with cancer, the numbers of OKT8+ lymphocytes did not changed. So the elevation of percentage of OKT8+
    lymphocytes was caused by the decrease of the numbers of OKT4+ lymphocytes. Then for units of the subsets of lymphocytes as parameter, absolute numbers were better than percentage.
    According to the examination of the change of the subsets in radiation therapy, it was supposed that the best preoperative dose was 2000 rads.
  • 深水 浩三
    1985 年 88 巻 4 号 p. 449-454
    発行日: 1985/04/20
    公開日: 2008/03/19
    ジャーナル フリー
    Glycoconjugates in normal respiratory mucosa were studied histochemically. PAS, HID-AB staining and lectins-HRP methods were used to stain the specimens, which have been obtained from volunteers or the patients with laryngeal cancer. The results obtained were as follows.
    The larger amounts of sulfo-mucin were observed in laryngeal mucosa than it in nasal mucosa. However, sialo-mucin were shown much more dominant in nasal mucosa compared with it in laryngeal mucosa. Some sugar residues stained by RCA, PNA, SBA were detected in several different grades in goblet cells and mucous cells of mucosal gland, while serous cells were unreactive. UEA-I, a lectin specific to fucosyl residue, were observed at all mucous cells and serous cells in nasal gland, whereas only some mucous cells in laryngeal mucosa. WGA and DBA applied to the specimens of this series were also shown.
    In the squamous epithelium of laryngeal mucosa, their middle layers were stained characteristically with PNA, SBA and UEA-I.
  • 三部 重雄, 小林 一豊, 朝倉 光司, 形浦 昭克
    1985 年 88 巻 4 号 p. 455-462
    発行日: 1985/04/20
    公開日: 2008/03/19
    ジャーナル フリー
    The malignant tumors of the ear are rare. Most of these are squamous cell carcinoma. We reported a 39-years-old woman of malignant melanoma in the right external auditory canal who suffered the generalized vasculitis during course.
    After admission multiple coin lesions were revealed by a chest X-ray film which suspected pulumonary metastasis of melanoma, so a course of immunochemotherapy (Picibanyl, ACNU, DTIC and VCR) was given. The course after immunochemotherapy was improving satisfactory. The pulumonary lesions were getting better and general fatigability was lessened gradually. But on the 68th hospital day the patient experienced abdominal pain and bloody stool abruptly, and on the 80th hospital day the patient was died by DIC.
    The autopsy revealed no melanoma cells in the any organs including the ear. But vasculitis was proven in the small intestine, colon, muscle, pulumonary tissues and also in the external auditory canal.
  • 白血球粘着阻止(LAI)試験による検索
    氷見 徹夫
    1985 年 88 巻 4 号 p. 463-476
    発行日: 1985/04/20
    公開日: 2008/03/19
    ジャーナル フリー
    The Leukocyte Adherence Inhibition (LAI) test was used to study anti-tumor immunity in patients with head and neck squamous cell carcinoma. 25.9 percent of the patients with laryngeal cancer (7 of 27) and 33.3 percent of the patients with maxillary cancer (6 of 18) had positive LAI responses to soluble extract from the same type tumor.
    LAI responses were demonstrated in KLH sensitized mice peritoneal exudate mononuclear cells and human peripheral mononuclear cells.
    The serum blocking factor (B. F.) was not detected in any LAI reactive patients with laryngeal and maxillary cancer. B. F. was measured by the inhibition rate of LAI responses of KLH-sensitized mononuclear cells. The lower reactivity of LAI responses and the higher percentage of the B. F. positive rate were shown in patients at a more advanced clinical stage.
    To elucidate the nature of B. F., serum immune complexes (I. C.) were investigated by the anti-C3 solid phase ELISA. 20.7 percent of the patients with laryngeal cancer (6 of 29) and 31.3 percent of the patients with maxillary cancer (5 of 16) had positive serum I. C.. Similary, patients at a more advanced clinical stage had higher positive rates of serum I. C.. I. C.s in patients with positive B. F. were significantly higher than in those with negative B. F.. This suggests that serum B. F. is related to serum I. C..
  • 岡本 美孝, 今野 昭義, 西平 茂樹, 花沢 秀, 寺田 修久, 波田野 洋一, 戸川 清
    1985 年 88 巻 4 号 p. 477-485
    発行日: 1985/04/20
    公開日: 2008/03/19
    ジャーナル フリー
    Cisplatin (CDDP) was microcapsulated with ethylcellulose. These CDDP-microcapsules (CDDP-m. c.) were prepared based on the principles of coacervation, with modification.
    Sustained release of CDDP from the microcapsule, especially non-protein-bound CDDP which has antitumor activity, was clearly demonstrated by an invitro test.
    By a bioassay method, it was proved that biological activity of CDDP was not affected by the micro-encapsulation process here employed.
    When CDDP-m. c. were infused into the maxillary artery of patients with carcinoma of maxillary sinus or oral cavity, CDDP level in the circulating blood was significantly lower than those of patients administered non-encapsulated CDDP intravenously. However, significantly high CDDP concentration in tumor tissue was found in the patients treated with CDDP-m. c.
    These results suggest that selective arterial infusion of CDDP-m. c. could exerts an intensive topical antitumor effects through microinfarction effect and prolonged drug release with decreased systemic side effect.
  • 伊賀 司, 稲留 欣一, 瀬尾 攝, 荻野 敏
    1985 年 88 巻 4 号 p. 486-491
    発行日: 1985/04/20
    公開日: 2008/03/19
    ジャーナル フリー
    Nasal respiratory resistance was measured in twenty five healthy adults by oscillation method using microrhinography (Chest, TUC-5000) for study of nasal cycle and objective diagnosis of nasal provocation test.
    Twelve persons (48%) showed the inversion of the dominant side during three hours.
    It seemed that this phenomenon were nasal cycle.
    Even if nasal cycle was appeared, the bilateral nasal respiratory resistance showed only slight changes.
    Considering by the change of the nasal respiratory resistance after fifteen minutes, it was reasonable that positive criterion in nasal provocation test was twice or more rise of the nasal respiratory resistance levels.
  • 浜口 富美
    1985 年 88 巻 4 号 p. 492-501
    発行日: 1985/04/20
    公開日: 2008/03/19
    ジャーナル フリー
    Various factors related to the mechanism of allergic reaction in the nasal mucosa were analyzed in 95 adult patients with nasal allergy; 75 were induced by house dust and 20 were induced by Japanese cedar pollen. Nasal mucosa of the inferior turbinate was excised from 20 patients with house dust allergy. Ten of the patients with non-atopic hypertrophic rhinitis were used as a control. The number of basophilic cells on the surface of the nasal mucosa in the allergic group was significantly larger (p<0.001) than that in control. In the patients induced by cedar pollen, the number increased significantly (p<0.001) in the season with apparent clinical symptoms. All the ratios of IgE antibody(Ab)/IgE, IgE-Ab/albumin and IgE-Ab/IgG on the surface of the nasal mucosa were higher than those in the deep layer, suggesting the local predominance of IgE on the surface of the nasal mucosa. In the patients induced by cedar pollen, IgE-Ab increased in the season. On the surface, histamine content in the allergic group was significantly larger (p<0.005) than that in control, but in the deep layer, there was no significant difference between both groups. In the rate of histamine release, there was no significant difference between the surface and the deep layers. The threshold of nasal mucosal sensitivity in the allergic group was significantly lower (p<0. 001) than that in control.
    All these findings suggest that kinetics of various factors on the surface of the nasal mucosa would be much related to the mechanism of allergic reaction; the number of basophilic cells, local production of IgE antibody and the threshold of nasal mucosal sensitivity.
  • 増野 精二
    1985 年 88 巻 4 号 p. 502-511
    発行日: 1985/04/20
    公開日: 2008/03/19
    ジャーナル フリー
    For the purpose of developing the optimal combination regimen with Cisplatin (CPDD) and Peplomycin (PEP), fundamental studies of the combination were made.
    In the experiment No. 1, Ehrlich ascites carcinoma, known to be sensitive to both cisplatin and peplomycin, was used as the experimental tumor. Six-week-old, male ICR mice, transplanted with this experimental carcinoma, were divided into 35 groups: the groups dosed with 8, 4 or 2mg/kg of cisplatinum, or 32, 16 or 8mg/kg of peplomycin alone, respectively, or in combination, with cisplatinum as the preceding medication or peplomycin as the preceding medication or both administered simultaneously, a control group, and an intact control group. Both drugs were administratered intraperitoneally by one-shot injection. The mean survival time and median survival time were used as parameters for the evaluation of drug effect.
    In the experiment No. 2, head and neck squamous cell carcinoma transplanted to nude mice was used as the experimental tumor. The doses of the drugs were the intermediate of those applied in the experiment No. 1; that is, cisplatin 4mg/kgr and peplomycin 16mg/kg. Both drugs were administered intraperitoneally by one-shot injection. The mice were divided into 4 groups: control group, a group receiving peplomycin alone, a group receiving cisplatin alone and a group receiving the combination of the two drugs. The antitumor effects of the drugs were evaluated by the changes in the volume of the tumor in each group.
    The results indicated following conclusions.
    1. In the experiment No. 1, the combination of CPDD and PEP proved to exert a synergistic effect on more than half of the so treated groups. The administration of CPDD as the preceding medication was found to be more effective but less toxic than the other two administration sequence, and its difference from the findings in the group treated with PEP as the preceding medication was of statistical significances.
    2. In the experiment No. 2, the antitumor effects were highest in the group of combination treatment, and it was confirmed by the statistical analysis that the synergistic effects were obtained by the combined administration of both drugs.
    3. The mechanism of the observed synergism in these combined administration was discussed from following three viewpoints: pharmacodynamic, cell kinetic and DNA repair; and the last seemed to be of the most probable mechanism.
    4. From the findings in these preclinical studies and the mechanism of the synergism, the administration of CPDD as the preceding medication seemed to be the desirable medication sequence.
  • 藤井 正人
    1985 年 88 巻 4 号 p. 512-519
    発行日: 1985/04/20
    公開日: 2008/03/19
    ジャーナル フリー
    Cisplatin (CDDP) and peplomycin (PEP) are effective new agents for chemotherapy of head and neck cancer. And the combination therapy of the two drugs is considered to have good synergistic effects. Despite the observances made in both basic experiments as well as in clinical trials, the mechanism of synergism, however, remains unknown. Experimental studies, therefore, were made to solve the mechanism of synergism.
    First, combination therapy with CDDP and PEP was performed, using Ehrlich ascites carcinoma, in actual clinical dose schedule. CDDP was administered intra-peritoneally at day 0, and PEP 48 hours later (day 3). This combination therapy with CDDP and PEP proved to have good synergistic effects.
    As for the mechanism of synergism, one of the hypotheses was as follows. It was thought that nonprotein-bound CDDP with anti-cancer activity might be released from protein-bound CDDP when PEP is administered in combination. But after the experiment in vitro, using dog plasma, my conclusion is that CDDP is bound to plasma protein irreversivelythus denying this hypothesis.
    The next study for the mechanism was to see the effects against cell cycle progression by flowcytometry process. It was found that at an early stage after single administration of CDDP, cell cycle progression was delayed in S phase and blocked in G2 phase thereafter. Forty-eight hours after administration of CDDP, cells accumulated in G2-M phase. This change, however, proved to be reversible because cells returned to normal progression pattern 96 hours after single administration of CDDP. As for PEP in single administration, cell cycle progression was blocked in G2-M phase at an early stage; it was also reversible. In the case of combination therapy, cell cycle progression pattern proved irreversible, and damaged cells (debris) appeared at low channels. It is, therefore, assumed that cells are seriously and irreversibly damaged in combination therapy.
    As result of the zoregoing studies, one of the mechanism of synergism is thought to be as follows:
    After administration of CDDP, cells are accumulated in G2-M phase. PEP is administered 48 hours after CDDP treatment when cells are most accumulated in G2-M phase. This brings about PEP's most efficient cytocydal effects since PEP has high sensitivity against cells in G2-M phase. This may explain one of the mechanism of synergism. And the high clinical respose rate in combination therapy of CDDP and PEP - when the former is administered first and the latter 48 hours after CDDP treatment - may be due to this mechanism.
  • 原口 茂徳
    1985 年 88 巻 4 号 p. 520-535
    発行日: 1985/04/20
    公開日: 2008/03/19
    ジャーナル フリー
    99mTcO4- and 67Ga-citrate scintigraphies were applied to 114 cases of parotid tumor patient. Diagnostic evaluation of these methods was estimated especially from the viewpoint of preoperative malignancy grading, comparing with their clinical figures and histopathological findings.
    In 99amTcO4- scintigram, parotid neoplasms often showed focal defect (81%). Otherwise, adenolymphoma actively accumulated 99mTcO4- nearly two third cases. Only mature typed adenolymphoma which comprised macroscopic follicular cyst, sometimes presented focal defect. Well differentiated type of mucoepidermoid tumors hardly presented different images from contralateral parotid glands.
    In 67Ga-citrate scintigram, benign neoplasms showed symmetrial or focally defective appearances (72%). On the contrally, high grade malignant tumors indicated high incidence of focal hot nodule (75%). Low grade malignancy tumors, which comprise acinic cell tumor and well differenciated mucoepidermoid tumor, also showed focal hot scintigram or diffuse increased uptake in high rate (92%).
    This study proved that 99mTcO4- and 67Ga-citrate scintigraphies are sufficiently useful to diagnose parotid tumor malignancy in advance to surgical operation.
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