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Volume 140 (1983)
- Issue 4 Pages 339-
- Issue 3 Pages 225-
- Issue 2 Pages 109-
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Volume 140, Issue 1
Displaying 1-14 of 14 articles from this issue
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HITOSHI ONODERA, GORO TAKADA, KEIYA TADA, ROBERT J. DESNICK1983 Volume 140 Issue 1 Pages 1-13
Published: 1983
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSONODERA, H., TAKADA, G., TADA, K. and DESNICK, R.J. Microautoradiographic Study on the Tissue Localization of Liposome-Entrapped or Unentrapped 3H-Labeled β-Galactosidase Injected into Rats. Tohoku J. exp. Med., 1983, 140, (1), 1-13-The localization of intravenously injected liposome-entrapped or unentrapped 3H-β-galactosidase in various tissues of rats was investigated by microautoradiography. The microautoradiographic silver grains, indicating the uptake of both forms of the enzyme were observed in all of the rat tissues studied, such as the liver, kidneys, spleen, lungs, heart, muscle and brain. The liver was found to be the most active in taking up both forms of the enzyme and probably Kupffer cells were primarily involved in the uptake of the enzyme. Also in the brain, the uptake of both forms of the enzyme was observed, though far less extensively, through the blood-brain barrier. The uptake of the enzyme in the brain was somewhat greater in the gray matter than in the white matter.View full abstractDownload PDF (11706K) -
KOICHI HIRATA, KOJI SHIRAMATSU, TOMOAKI USUI, YUTAKA YOSHIDA, AARON E. ...1983 Volume 140 Issue 1 Pages 15-28
Published: 1983
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSHIRATA, K., SHIRAMATSU, K., USUI, T., YOSHIDA, Y., FREEMAN, A.E. and HAYASAKA, H. Modulation of Fetal Mouse Liver Cells Cultured on a Pigskin Substrate. Tohoku J. exp. Med., 1983, 140, (1), 15-28-Mouse fetal liver cells cultured on a pigskin epidermal substrate grew for 7 weeks. Different enzymes and proteins, i.e. gamma-glutamyl transferase (GGT), nonspecific esterase (NE), lactic dehydrogenase (LDH), alpha-fetoprotein (AFP), and albumin were studied histochemically and/or biochemically. The activity of GGT was high at the beginning of culture and then decreased rapidly. The activities of NE and LDH were high during the culture. Release into the media and localization of AFP suggested active synthesis during the early stages. AFP levels gradually decreased and could be demonstrated only in trace amounts after 3-4 weeks of culture. On the other hand, the production of albumin was weakly evident early and became more and more evident after the second week in culture. Hydrocortisone modulated AFP and albumin production. The effect of hydrocortisone was to prolong expression of AFP and to reduce expression of albumin. Electron microscopic observations showed that the cultures consisted of organelle-rich parenchymal cells associated with the pigskin basement membrane by pseudopod-like structures. These results indicate that fetal mouse parenchymal cells were cultured and modulated on a pigskin epidermal substrate.View full abstractDownload PDF (6945K) -
KAZUO ABE, NOBUO MATSUURA, NAOKI FUKUSHIMA, YACHIYO NOHARA, HIROKO FUJ ...1983 Volume 140 Issue 1 Pages 29-34
Published: 1983
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSABE, K., MATSUURA, N., FUKUSHIMA, N., NOHARA, Y., FUJITA, H., FUJIEDA, K. and KATO, T. Plasma Prolactin Response to Thyrotropin Releasing Hormone in Children with Newly Diagnosed Insulin Dependent Diabetes. Tohoku J. exp. Med., 1983, 140 (1), 29-34-The basal and TRH-induced prolactin (PRL) and TSH secretions were examined in 14 children aged 4 to 13 years with newly diagnosed insulin dependent diabetes (IDD) within three weeks after diagnosis. Basal PRL levels did not differ from the values of control. In response to TRH, 6 out of 14 patients showed an exaggerated PRL response and 8 a normal PRL response. The basal and TRH-induced TSH secretions were normal, while plasma triiodothyronine (T3) and thyroxine (T4) concentrations were significantly (p<0.001, p<0.02) lower in patients than in controls. These findings suggest that a significant proportion of children with newly diagnosed IDD has an exaggerated PRL response to TRH, and TSH secretion remains unchanged despite significant decreases of circulating thyroid hormone levels.View full abstractDownload PDF (497K) -
HIROSI SATO, HIDEO ARAI, HIROMI NAGAI, MASAYUKI ITO, KEN SATOH, NOBUKO ...1983 Volume 140 Issue 1 Pages 35-43
Published: 1983
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSATO, H., ARAI, H., NAGAI, H., ITO, M., SATOH, K., KUMANO, N., MOTOMIYA, M. and KONNO, K. Chemical Analyses and Antitumor Activity of Hydrosoluble Substances from Mycobacterium bovis, Strain BCG. Tohoku J. exp. Med., 1983, 140 (1), 35-43 - Hydrosoluble substances from BCG were prepared by cold water extraction and by hot phenol-water extraction. Chemical analyses revealed that both of them were derived from cytoplasm. The cold water extract (CWE) was effective in the treatment of C3H/He mice which had received an intraperitoneal inoculation of a syngeneic ascites hepatoma, MH134. The growth of a graft in footpad of mastocytoma P815 in CDF1 mice was retarded by intraperitoneal injections of CWE. A peptidoglycan from cell wall prepared by digestion with lysozyme exerted no antitumor activity in the same experimental condition as for the evaluation of antitumor effect of CWE. These results indicate that the antitumor activity of CWE was not due to the presence of a cell wall component.View full abstractDownload PDF (874K) -
YASUE FUKUSHI, TSUNEHIKO SUZUKI1983 Volume 140 Issue 1 Pages 45-51
Published: 1983
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSFUKUSHI, Y. and SUZUKI, T.S. Effects of Homopantothenic Acid on Intestinal Smooth Muscles. Tohoku J. exp. Med., 1983, 140 (1), 45-51 -The effects of homopantothenic acid (HOPA) on intestinal smooth muscles were investigated and compared with those of γ-aminobutyric acid (GABA). HOPA contracted the rat ileum and produced a biphasic response (relaxation followed by contraction) in the guinea-pig ileum and taenia coli. Half-maximal effects were obtained with 0.3-0.9nM HOPA and maximal responses with 2-4nM in the rat and the guinea-pig ileum. In the guinea-pig taenia, half-maximal effects were obtained with 0.2-0.25μM HOPA and maximal responses with 2-4μM HOPA. Tachyphylaxis occurred with higher concentrations. All these responses were not significantly affected by tetrodotoxin (TTX), atropine, diphenhydramine or metiamide. The relaxation elicited by HOPA was not reduced by simultaneous application of phentolamine and propranolol. Unlike HOPA, GABA produced a biphasic response (a relaxation followed by a contraction) in the guinea-pig ileum, which was almost abolished by TTX. These results suggest that HOPA mainly acts on intestinal smooth muscle directly, whereas GABA does indirectly through nerves.View full abstractDownload PDF (617K) -
SADAYOSHI ITO, MASAHIDE SEINO, MINORU YASUJIMA, KEISHI ABE, KAORU YOSH ...1983 Volume 140 Issue 1 Pages 53-58
Published: 1983
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSITO, S., SEINO, M., YASUJIMA, M., ABE, K. and YOSHINAGA, K. Effects of Nifedipine on the Renal Vascular Responses and Blood Pressure Responses to Norepinephrine and Angiotensin II in the Anesthetized Rabbit. Tohoku J. exp. Med., 1983, 140 (1), 53-58-Effects of a Ca-antagonist, nifedipine, on renal vascular responses and systemic pressor responses to angiotensin II and norepinephrine were studied in anesthetized rabbits. The changes of renal blood flow were estimated by an electromagnetic flowmeter. After the intravenous administration of nifedipine (50μg/kg), mean blood pressure decreased from 116±3.1mmHg to 102±3.4mmHg (p<0.001). Renal vascular responses to angiotensin II were attenuated significantly after the administration of nifedipine, but not changed to norepinephrine. Pressor responses to angiotensin II at a low dose were significantly diminished after the administration of nifedipine (p<0.05), whereas it had no effect at higher doses. Nifedipine tended to suppress the increase in arterial pressure induced by norepinephrine, but the changes were not statistically significant. These results suggest that calcium influx in the vascular smooth muscle cells is not likely to be a common pathway in the vasoconstrictor reaction to angiotensin II and norepinephrine in anesthetized rabbit.View full abstractDownload PDF (670K) -
MORIHIRO OHARA, MASAMI SHIRADO, MASAYUKI MIYATA, HIROFUMI SAKUMA, MINO ...1983 Volume 140 Issue 1 Pages 59-66
Published: 1983
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSOHARA, M., SHIRADO, M., MIYATA, M., SAKUMA, H., NANAMIYA, M., YOSHIDA, N., YOSHIDA, H. and KASUKAWA, R. Natural and Antibody-Dependent Cellular Cytotoxicity of Polymorphonuclear Luekocytes. Tohoku J. exp. Med., 1983, 140 (1), 59-66-Cytotoxic activity of polymorphonuclear leukocytes (PMN) in the peripheral blood of patients with various diseases was demonstrated to K562 cells (natural cytotoxicity, NC) and the antibody-coated P815 cells antibody- dependent cellular cytotoxicity, ADCC, using a 51Cr-release method. The NC values of normal PMN were lower than those of normal lymphocytes with mean values of 5.0% and 30%, respectively. The NC values of patients' PMN were also lower in malignancy, chronic hepatitis and connective tissue diseases. The ADCC values of normal PMN were moderately high with a mean value of 16.0%, which was almost a half of normal lymphocytes. Higher ADCC values of PMN were found in patients with chronic hepatitis, SLE and Behcet's disease, and in these cases the ADCC values of their lymphocytes were extremely low. The supernatants of PMN mix-cultured with unlabeled K562 or the antibody-coated P815 cells were fairly cytotoxic to both cells, though the similar supernatants of lymphocytes were cytotoxic only to K562 cells, but not to the antibody-coated P815 cells.View full abstractDownload PDF (691K) -
ITARU KIMURA, D. RAM AYYAR, JAMES C. MCVEETY1983 Volume 140 Issue 1 Pages 67-71
Published: 1983
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKIMURA, I., AYYAR, D.R. and MCVEETY, J.C. Saphenous Nerve Conduction in Healthy Subjects. Tohoku J. exp. Med., 1983, 140 (1), 67-71-Saphenous nerve conduction was studied in 40 healthy subjects utilizing a slight modification of the method described by Wainapel et al. The mean values obtained were as follows: distal sensory latency, 2.9+0.3msec for 10cm distance; conduction velocity, 46.6+3.5m/sec; and amplitude of sensory nerve action potential, 10.7+4.3μV. The technique should be useful in the electrodiagnostic differentiation between lumbar root (L3, L4) lesions and postganglionic lesions such as lumbar plexus and femoral nerve lesions and entrapment neuropathy of the saphenous nerve.View full abstractDownload PDF (415K) -
HIROSHI YOSHIDA, MIKIO SATO, SUSUMU WATANABE, TOMOE NISHIMAKI, TAKAO M ...1983 Volume 140 Issue 1 Pages 73-79
Published: 1983
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSYOSHIDA, H., SATO, M., WATANABE, S., NISHIMAKI, T., MORITO, T., KASUKAWA, R. and NAKAI, M. Clq and Immune Complexes in Liver Cirrhosis Sera. Tohoku J. exp. Med., 1983, 140 (1), 73-79-The highest mean value of the serum Clq concentrations among chronic liver diseases was obtained in patients with liver cirrhosis (LC). Clq serum levels seemed to increase along the progression of liver damage. Serum levels of Clq and CH50 in patients with LC and systemic lupus erythematosus (SLE) were simultaneously estimated. No correlation between Clq and CH50 levels was observed in LC sera, while a significant correlation was demonstrated in SLE sera. It could be suggested that the mechanism for the low CH50 in LC sera seemed different from that in SLE sera, and that the activation of classical complement pathway was not the predominant cause in LC sera. Correlations of serum levels between immune complexes and Clq were examined in patients with LC and SLE. It could be deduced from the results of a positive correlation in LC sera and the reverse tendency in SLE sera that the significances of Clq and immune complexes in LC sera differed from those in SLE sera.View full abstractDownload PDF (707K) -
KENICHI SHIMADA, WAKAKO TAKAHASHI, ARATA WATANABE, KENICHI MATSUNO, OT ...1983 Volume 140 Issue 1 Pages 81-88
Published: 1983
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSHIMADA, K., TAKAHASHI, W., WATANABE, A., MATSUNO, K. and HIGASHI, O. Further Studies on the Effects of Flavine Adenine Dinucleotide on the Platelet Functions. Tohoku J. exp. Med., 1983, 140 (1), 81-88 Flavine adenine dinucleotide (FAD) may inhibit not only the aggregation but also ATP release of platelets in vitro. FAD did not inhibit the synthesis of thromboxane from 1-14C-arachidonic acid by platelets. FAD was found to be hydrolyzed to FMN and AMP by a plasma enzyme, the activity of which in normal adults being estimated as 32.6+9.1mμM/ml plasma/min and AMP, thus produced, and/or FAD might compete for the receptor sites on the platelet surface. This could be the mechanism of the inhibitory effect of FAD upon the platelet functions. The intravenous drip infusion of FAD in a dose of 0.2mg/kg within 30min was followed by no marked decrease of platelet aggregation, probably because the plasma concentration of FAD and/or AMP did not reach the levels high enough to suppress the platelet functions.View full abstractDownload PDF (714K) -
SHIGEO SATAKE, NOBORU OTOTANI, MAMORU ISEMURA, ZENSAKU YOSIZAWA1983 Volume 140 Issue 1 Pages 89-96
Published: 1983
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSATAKE, S., OTOTANI, N., ISRMUEA, M. and YOSIZAWA, Z. Enzymatic Determination of Urinary Glycosaminoglycans from Orthopedic Patients. Tohoku J. exp. Med., 1983, 140 (1), 89-96-Crude glycosaminoglycan (GAG) fraction was directly precipitated with cetylpyridinium chloride without prior dialysis of urine of orthopedic patients. The crude GAG fraction was then fractionated with trichloroacetic acid (TCA). The TCA-insoluble peptide-bound GAG fraction thus obtained was treated with alkali to eliminate the peptide moiety for enzymatic analysis. The GAG compositions of this fraction and the TCA-soluble fraction were determined by digestion with mucopolysaccharidases (chondroitinase AC, chondroitinase B, chondroitinase C, heparitinase and Streptomyces hyaluronidase). When the amount of the crude GAG fraction was small, no significant amount of the TCA-insoluble peptide-bound GAG fraction was obtained. The GAG composition of this case was also determined by the same procedures after direct alkali-treatment of the crude GAG fraction. The data indicated that the proportion of the TCA-insoluble peptide-bound GAG fraction was very small. The alkali-treated TCA-insoluble peptide-bound GAG fraction contained a larger proportion of heparan sulfate than the TCA-soluble GAG fraction. It was clearly demonstrated that the patients with Werner's syndrome and mucopolysaccharidosis I-S (Scheie) excreted large amounts of hyaluronic acid and dermatan sulfate respectively, into urines. It was indicated in most cases that major urinary GAG were chondroitin 4-sulfate, chondroitin 6-sulfate plus chondroitin and heparan sulfate, while minor ones were dermatan sulfate and hyaluronic acid. In addition, the data suggested a wide range of the degree of desulfation of urinary GAG, and the presence of significant amounts of keratan sulfate plus acidic glycopeptides in the urinary GAG fractions. The present data provided more precise information on urinary GAG from orthopedic patients than those reported previously.View full abstractDownload PDF (789K) -
RIKURO SASAKI, TADASHI KUROKAWA, TEIJI KOBAYASI, SHOZO TERO-KUBOTA1983 Volume 140 Issue 1 Pages 97-104
Published: 1983
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSASAKI, R., KUROKAWA, T., KOBAYASI, T. and TERO-KUBOTA, S. Influences of Sex and Age on Serum Ascorbic Acid. Tohoku J. exp. Med., 1983, 140 (1), 97-104-The concentrations of total ascorbic acid, reduced and oxidized forms of ascorbic acid, the ESR intensity of ascorbate radical, and the ratio of oxidized form of ascorbic acid to total ascorbic acid (DAsA/AsA) were estimated on 217 healthy controls, whose ages ranged from 12 to 96 years, in order to examine influences of sex and age. The concentration of total ascorbic acid was higher in females than in males throughout all age classes, but the oxidized form did not show a sex difference. Then it was found that the reduced form was higher in females than in males throughout all age classes. The concentrations of total ascorbic acid and reduced and oxidized forms of ascorbic acid, and the ESR intensity declined with age, but the DAsA/AsA ratio increased with age.View full abstractDownload PDF (646K) -
MASAO ENDOH1983 Volume 140 Issue 1 Pages 105-106
Published: 1983
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSENDOH, M. Differential Effects of 8-Bromo-Cyclic GMP on the Basal Contractile Force and β-Adrenoceptor-Mediated Positive Inotropic Action in the Canine Atrium. Tohoku J. exp. Med., 1983, 140 (1), 105-106-8-Bromo-cyclic GMP caused a negative inotropic action on the canine atrial muscle, whilst it did not affect the positive inotropic action of phenylephrine mediated via β-adrenoceptors. These findings are in line with the hypothesis that the muscarinic receptor stimulation may exert functional changes in the heart via two different intracellular biochemical processes: one involving cyclic AMP and the other involving cyclic GMP. 8-Bromo-cyclic GMP mimics the latter but not the former mechanism in canine atria.View full abstractDownload PDF (225K) -
KENJI MIZUNO, MITSUHIRO GOTOH, JUNICHIROH MATSUI, SYUICHI NAGASAWA, SO ...1983 Volume 140 Issue 1 Pages 107-108
Published: 1983
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSMIZUNO, K., GOTOH, M., MATSUI, J., NAGASAWA, S. and FUKUCHI, S. Acute Effects of Captopril on Serum Angiotensin-Converting Enzyme Activity, the Renin-Aldosterone System and Blood Pressure in Patients with Sarcoidosis. Tohoku J. exp. Med., 1983, 140 (1), 107-108-Captopril, an orally active angiotensin-converting enzyme (ACE) inhibitor, was administered to three patients in an active stage of sarcoidosis. The serum ACE level, aldosterone concentration in plasma and blood pressure decreased rapidly after administration, while plasma renin activity was not significantly changed. It is suggested that inhibition of ACE by captopril offers a possible therapeutic approach to the treatment of sarcoidosis.View full abstractDownload PDF (224K)
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