The effect of 4, 4'-methylenedianiline on the adrenocortical secretion in the rat was investigated. Under sodium pentobarbital anesthesia, adrenal venous blood was collected through a polyethylene cannula inserted into the adrenal vein and analyzed for corticosterone. 4, 4'-Methylenedianiline dihydrochloride was administered intravenously in a dose of 100mg/kg. In non-hypophys-ectomized rats, the increased corticosterone secretion in response to surgical stress caused by the adrenal vein cannulation was markedly depressed following the injection of methylenedianiline. A low secretion rate of corticosterone persisted for 30 minutes thereafter. In hypophysectomized rats, the increased corticosterone secretion produced by intravenous infusion of ACTH was also markedly depressed by injection of methylenedianiline. It is thus concluded that in the rat the adrenal corticosterone secretion is depressed by 4, 4'-methylenedianiline and that this inhibitory effect on adrenal corticosterone secretion is due to a direct action of the drug on the adrenal cortex.
Differential renal function test was made on thirty-four hypertensive patients without abnormalities of renal arteries and thirteen patients with renovascular hypertension. The diagnostic significance of these studies was evaluated, and new criteria consisting of two indices, tubular rejection fraction ratio (TRFR, Rapoport) and left/right ratio of UK/UNa were presented. In patients without abnormalities of renal arteries both indices of TRFR and left/right ratio of UK/UNa remained close to 1.0. In four of ten patients with unilateral stenosis of renal artery, the values of TRFR overlapped the normal range. In contrast, the left/right ratios of UK/UNa a were distinctly deviated from 1.0. In one of three patients with bilateral renal artery stenosis, both indices showed a pattern of the unilateral stenosis, suggesting that the stenosis of renal artery was severer in degree on one side than on the other. It was found that, although TRFR had a fairly good diagnostic value, false negative results could not be completely avoided and that since UK/UNa was higher on the side of stenosis, the left/right ratio of UK/UNa was a helpful para-meter for the diagnosis of the unilateral stenosis of renal artery. The diagnostic value of the differential renal function test was found to be more reliable when TRFR and left/right ratio of UK/UNa were simultaneously evaluated.
A new serological technique was introduced into blood grouping of blood-stains and other test specimens. In this method, latex-adsorbed agglutinin was prepared by mixing polyvinyl-toluene latex particles of 2.015μ in diameter with antiserum. Accurate blood grouping was possible with the agglutinin-adsorbing latex particles directly even on a small piece of blood-or saliva-stained fibrils in only about ten minutes. The described technique was recommended for wide use not only in blood group determinations, but also in general serological tests.
A case is reported of a newborn infant displaying symptoms resembling cat cry syndrome who had mental and growth retardation, and microcephaly, epicanthus, antimongoloid slant, low-set ears, hypertelorism, moon face, short neck, and cardiac failure. The chromosomal abnormality consisted in a partial deletion of the distal portion of the short arm (of a chromosome) of the 4-5 group.
In recent years, the outcome of tumor-growth has increasingly been interpreterl from the tumor-host relationship. Some tumor-factors such as maligiianey, antigenicity of tumor, etc., contribute to determining the relation. Our immuxiological research on experimental and human cancers revealed appearance of cancer antigen and loss of organ specific antigen in cancer tissues. Several host factors may also contribute to host resistance, such as hormonal regulation. reticuloendotheiial activity, antibody titers, etc. Among the above-mentioned factors, none can be independently indicative of the host resistance. The author's eperienee on cancer auto-transplantation shows that, although it does not promise a better prognosis, it is useful in the evaluation of the degree of relapse and metastasis of tumors in the abdominal cavity. The author believes that a safe and simple aid for the diagnosis of cancer relapse is offered by the auto-transplantation. Immunological aspect of tumors and its relationship to cancer auto-transplantation is also discussed.
Electron microscopic observation of reticuloendothelial sytem was made in untreated albino rabbits and typhoid vaccine injected rabbits. On the bas_??_s of the findings obtained from panoramic electron microphotographs, the reticuloendothelial cells in the splenic red pulp could be classified into the sinus endothelial cell, fixed reticulum cell, ingesting reticulum cell and reticulum cell around the sheathed artery. Furthermore, the fixed reticulum cell was subdivided into two types by their location in the medullary cord; that is, the reticulum cell situated in the periphery of the cord, covering the outer surface of sinus basement membrane, and the cell situated in the center of the cord. Both fixed reticulumm cells formed the framework of the cord by extruding the cytoplasmic processes between other cells. The ingesting type of reticulum cell was different from the fixed reticulum cell and supposed to be derived from the immature lympho-reticular cell existing originally in the red pulp. The sinus endothelial cell showed some resemblance to the reticulum cell when stimulated, and cytoplasmic organellae were increased. In rabbits, the sheathed artery could be identified by the electron microscope and was surrounded by reticulum cells.
Purification of macroglobulin from a patient with Waldenström's macroglo-bulinemia was done through gel filtration, and water precipitability of each macroglobul in preparation was assayed by means of the Sia water test. Some other physicochemical properties of the macroglobulin preparation were also examined. Both above pH 9.2 and below pH 4.7, Sia test was negative and only a little dissociation was observed at faster sedimenting and minor component. Main peak of 15S did not show any dissociation by changing pH. Removal of IgG from Fr M-1 increased water solubility of macroglobulin. Neuraminidase treatment did not cause change in the Sia test, while it reduced the sedimentation coefficients of Fr M-2 from 14.9S and 24.6S to 13.3S. Decrease of sedimentation coefficients to 8.3S and 12.2S by urea treatment did not cause change in the Sia test. These results suggested that association of IgG with macroglobulin decreased water solubility of macroglobulin, and dissociation between macroglobulin and its subunits did not directly relate to water precipi-tability of the protein. The role of high contents of neutral sugar, threonine and serine in the precipitability of macroglobulin was discussed.
Two cases of typical Hartnup disease and six presumable carrier members through three generations were studied as the first cases in Japan. Cases 1 and 2 showed the typical clinical and laboratory findings of the disease. We carried out paperchrornatographic studies on urine specimens from parents, siblings, maternal first cousins, and maternal and paternal grandmothers and grandfathers. Two siblings, two maternal first cousins, and maternal and paternal grandfathers who were the first cousins had indicanuria and generalized aminoaciduria. But they had no symptoms suggestive of Hartnup disease. We think they may be carrier members of Hartnup disease.
Host-tumor relationship was discussed on the basis of the quantitative analysis of transplantability and survival curves in a series of transplantation experiments, in which various combinations of tumors and animals were examined. Acquired resistance against the growth of tumor could be increased in the hosts by various pretreatments such as heterotransplantation or BCG injection. The grade of resistance acquired was appreciated quantitatively.