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Volume 144 (1984)
- Issue 4 Pages 331-
- Issue 3 Pages 219-
- Issue 2 Pages 107-
- Issue 1 Pages 1-
Volume 144, Issue 3
Displaying 1-15 of 15 articles from this issue
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RYUICHI NAKAMURA, KENJI KOSAKA, RYOICHI HANAKAGO1984 Volume 144 Issue 3 Pages 219-225
Published: 1984
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSNAKAMURA, R., KOSAKA, K. and HANAKAGO, R. Arousal Response Induced by Postural Changes in Patients with Subacute Myelo-Optico-Neuropathy, “SMON.” Tohoku J. exp. Med., 1984, 144 (3), 219-225 - In reaction time (RT) experiments, shifts of arousal level induced by postural changes were examined in 10 normal subjects and 10 patients with SMON. Vocal RTs were recorded at neutral position (N) and facilitating position of the left arm (FA) or the left leg (FL). RT-ratios between two positions, FA/N and FL/N, were used as variables to estimate the arousing effect of facilitating positions. Also the correlations between these variables and several clinical signs such as patellar tendon reflex (PTR), sensory disturbance and dysesthesia were calculated. The grade of dysesthesia was strongly related to FL/N. It is assumed that proprioceptive input from the lower extremities causes abnormally high arousal response in patients with dysesthesia.View full abstractDownload PDF (647K) -
ATSUSHI KIMURA, SHIRO HAYASHI, MASAI KOSEKI, HIDEO KOCHI, KOICHI TSURU ...1984 Volume 144 Issue 3 Pages 227-236
Published: 1984
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKIMURA, A., HAYASHI, S., KOSEKI, M., KOCHI, H. and TSURUMI, K. Fractionation and Characterization of Urinary Heparan Sulfate Excreted by Patients with Sanfilippo Syndrome. Tohoku J. exp. Med., 1984, 144 (3), 227-236 -Urinary heparan sulfates (HS) from two siblings with mucopolysaccharidosis (MPS) III-B were fractionated by chromatography with Dowex 1 and Sephadex G-50. Their Mr ranged from 1600 to 8000, and 95% of them were included in the region less than 5000. Fractions with lower Mr contained larger amounts of O- and N-sulfates. The chemical analysis and deaminative cleavage of HS suggested that an intact HS molecule was composed of some blocks rich in GlcNAc and GlcUA and other blocks rich in GlcNS, IdUA and O-sulfate. GlcNAc-UA-GlcNS-UA-GlcNAc-UA-GlcNAc was found to be a major oligosaccharide of HS with Mr less than 1800. Trisaccharides, GlcNAc-GlcUA-aMan and GlcNAc-IdUA-aMan, were released from the nonreducing end of HS-oligosaccharides by deaminative cleavage. They carried 0-3 moles of ester sulfate. GlcNAc-IdUA-aMan was more sulfated than the other. The release of significant amounts of nonsulfated trisaccharide conform to the enzyme defect in this disease. Urinary HS obtained from another patient with MPS III were examined by the same way. Although the patient was not examined enzymatically, the structure of urinary GAG suggested a defect of α-N-acetylglucosaminidase in the patient.View full abstractDownload PDF (904K) -
TOMIO NARISAWA, PETER HERMANEK, MICHAEL HABS, DIETLICH SCHMÄHL1984 Volume 144 Issue 3 Pages 237-243
Published: 1984
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSNARISAWA, T., HERMANEK, P., NABS, M. and SCHMÄHL, D. Reduction of Acetoxymethyl-Methylnitrosamine-Induced Large Bowel Cancer in Rats by Indomethacin. Tohoku J. exp. Med., 1984, 144 (3), 237-243 - The nonsteroid anti-inflammatory drug indomethacin, a potent prostaglandin synthesis inhibitor, may play a role in preventing chemically-induced large bowel cancer development in rats. 250male Sprague-Dawley rats were given weekly intrarectal doses of 2mg/kg body weight of acetoxymethyl-methylnitrosamine (AMMN) in the first 10 weeks of the experiment to induce large bowel tumors. Experimental groups received a 0.001% aqueous solution of indomethacin ad libitum as drinking water for different time intervals. At autopsy in week 21, the indomethacin treatment in the first and second 10-week periods, or only in the second 10-week period significantly reduced the number of large bowel tumors compared to non-treatment control groups, while the treatment in the first 10-week period alone did not affect the tumor development. It was observed at autopsy in week 31 that the 10-week cessation of treatment after the effective treatments permitted the growth of tumors, but the treatment in the first and second 10-week periods was effective enough to supperss tumor apperance compared to other groups. It can be concluded that indomethacin has an antiproliferative activity on large bowel carcinogenesis.View full abstractDownload PDF (746K) -
TSUGUHIKO NAKAI, YASUNORI KUTSUMI, YOSHIKAZU SAKAMOTO, KOJI OIDA, SUSU ...1984 Volume 144 Issue 3 Pages 245-256
Published: 1984
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSNAKAI, T., KUTSUMI, Y., SAKAMOTO, Y., OIDA, K., MIYABO, S. and TAKEDA, R. The Effect of Insulin-Dextran Complexes on the Protein Synthesis in the Primary Monolayer Culture of Adult Rat Hepatocytes. Tohoku J. exp. Med., 1984, 144 (3), 245-256 - The purpose of the present study was to investigate the mechanisms of the long-term effects of insulin on protein synthesis in rat liver parenchymal cells. Three kinds of soluble and stable insulin-dextran complexes (I: Mr 150, 000; II: Mr 450, 000; III: Mr 2, 000, 000) were prepared. The effects of insulin or insulin-dextran complexes on protein synthesis were evaluated in the primary monolayer culture of adult rat liver. To avoid the effects of serum factors, the primary monolayer culture of adult rat liver in serum free medium was established. The cultured hepatocytes well maintained the metabolic and morphological characteristics of the adult rat liver. The incorporation of [14C] -leucine into trichloroacetic acid insoluble proteins and immunoprecipitates by anti-rat albumin serum were measured in cultured hepataocytes prepared either from the control or streptozotocin-induced rats. An addition of insulin-dextran complexes to the culture medium of the hepatocytes from diabetic rats stimulated the protein synthesis as well as the native insulin. The maximal effect of insulin-dextran complex (I) on protein synthesis was comparable to native insulin. However, insulin-dextran complex (II) caused a 67% stimulation of native insulin. Insulin-dextran complexes (III) induced only a slight increase of protein synthesis. Furthermore, an addition of insulin-dextran complexes into the medium caused a more tight cellular attachment to the dish and more extensive spreading. These results favor the view that the stimulation by insulin of protein synthesis in rat hepatocytes does not require the entry of insulin into cells.View full abstractDownload PDF (1867K) -
KOICHI KYONO, KAZUHIKO HOSHI, AKIRA SAITO, AKIRA TSUIKI, HIROSHI HOSHI ...1984 Volume 144 Issue 3 Pages 257-263
Published: 1984
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKYONO, K., HOSHI, K., SAITO, A., TSUIKI, A., HOSHIAI, H. and SUZUKI, M. Effects of Phospholipase A2, Lysophosphatidyl Choline, and Fatty Acid on the Acrosome Reaction of Human Spermatozoa. Tohoku J. exp. Med., 1984, 144 (3), 257-263 - An in vitro penetration assay employing zona-free hamster eggs was used to study the effects of phospholipase A2, lysophosphatidyl choline, and fatty acid on the acrosome reaction of human spermatozoa. Human spermatozoa were preincubated for 4hr in modified Biggers, Whitten, and Whittingham's medium (mBWW) containing a specific phospholipase A2 inhibitor, p-bromophenacyl bromide (p-BPB: 1×10-5-1×10-3M), lysophosphatidyl choline (LC: 5-500μg/ml), and arachidonic acid (AA: 5-500μg/ml), prior to the addition of zona-free superovulated hamster eggs. Eggs were examined microscopically 2 or 4hr later for evidence of swelling or decondensing sperm heads in the cytoplasm. Lysophosphatidyl choline increased penetration rates of spermatozoa from 43.2% (control) to 91.4% (LC: 50μg/ml). Arachidonic acid also increased penetration rates from 51.6% (control) to 87.0% (AA: 5μg/ml) and 80.5% (AA: 50μg/ml). p-BPB decreased penetration rates from 90.6% (1% dimethyl sulfoxide) to 16.0°C (1% dimethyl sulfoxide+p-BPB 1×10-4M). These results suggest that endogenous phospholipase A2 may break membrane phosphatidyl choline into lysophosphatidyl choline and fatty acid, when the acrosome reaction occursView full abstractDownload PDF (620K) -
SATORU ISHIKAWA, RYOSUKE NEMOTO, SHOURI KANOH, KATSUMI KOBAYASHI, SHOZ ...1984 Volume 144 Issue 3 Pages 265-271
Published: 1984
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSISHIKAWA, S., NEMOTO, R., KANOH, S., KOBAYASHI, K. and ISHIZAKA, S. Photodynamic Inactivation of Bladder Cancer Cells (MGH-U1) Sensitized with Acridine Orange and Irradiated by Argon Laser. Tohoku J. exp. Med., 1984, 144 (3), 265-271 - Photodynamic inactivation of bladder cancer cells (MGH-U1) was investigated in order to apply laser therapy to the treatment of bladder cancer. After stained with acridine orange (AO), the material cells were irradiated with argon laser. After 24hr incubation the survival was counted with a hemocytometer. The number of stained cells showed a less than 5% decrease and the number of irradiated (for 15min) unstained cells showed no decrease compared with untreated cells. Participation of singlet oxygen process in inactivation of MGH-U1 cells was confirmed by the use of D2O and NaN3. The result shows that argon laser at the low intensity and with short irradiation time has a sufficient cytocydal effect, suggesting the usefulness of photodyamic inactivation of argon laser with topical use of acridine orange in the treatment of bladder cancer.View full abstractDownload PDF (521K) -
YUTAKA WAKO, SHUICHI KIMURA1984 Volume 144 Issue 3 Pages 273-280
Published: 1984
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSWAKO, Y. and KIMURA, S. The Development of Glucose Metabolism in Infants of Diabetic Mothers. Tohoku J. exp. Med., 1984, 144 (3), 273-280 -The concentrations of plasma glucose, lactate and phosphoenolpyruvate carboxykinase (PEPCK) (EC 4.1.1.32) were studied in caesarian-delivered newborn rats of diabetic mothers (IDM) and normal mothers, at time-intervals up to 6hr after delivery. Glucose concentrations in plasma of cord blood of IDM were significantly higher than those in normal newborn rats. The glucose concentration of normal newborn rats decreased markedly during 1hr after delivery and thereafter increased gradually. However, the glucose concentration of IDM decreased to a minimum at 4hr after delivery and thereafter increased. Lactate concentration in plasma of cord blood was high at delivery. Plasma lactate concentration of normal newborn rats decreased rapidly during 2hr after delivery. However, plasma lactate concentration of IDM increased during 1hr after delivery and thereafter decreased markedly over the next 5hr. Hepatic soluble PEPCK activity in caesarian delivered rats was low at birth. The activity of normal infants increased after a lag of 2hr whereas the activity of IDM increased after a lag of 4hr. The concentrations of plasma glucose and hepatic PEPCK activity were measured as a function of time after the adminstration of glucose (5g/kg body weight) to caesarian-delivered newborn rats. The glucose concentration increased to a maximum at 2hr after administration and decreased markedly over the next 2hr. The development of enzyme activity was delayed in administered rats. The glucose concentration and hepatic PEPCK activity were measured as a function of time after the intraperitoneal injection of insulin (250mU/rat) into caesarian-delivered rats from diabetic pregnant rats. The injection of insulin decreased plasma glucose concentration in newborn rats. The development of PEPCK in IDM was advanced by the injection of glucagon.View full abstractDownload PDF (713K) -
MASAAKI ETO, KIYOSHI WATANABE, YASUNORI IWASHIMA, AKIZUKI MORIKAWA, TA ...1984 Volume 144 Issue 3 Pages 281-290
Published: 1984
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSETO, M., WATANABE, K., IWASHIMA, Y., MORIKAWA, A., TAKEBE, T. and ISHII, K. Elevation of Plasma High Density Lipoprotein-Cholesterol in Spontaneously Diabetic Chinese Hamsters. Tohoku J. exp. Med., 1984, 144 (3), 281-290 - The aim of this study is to define the change of plasma high density lipoprotein-cholesterol (HDL-C) in the spontaneously diabetic Chinese hamsters in the Asahikawa colony (CHA). These animals were divided into two groups according to fasting plasma glucose level; non-diabetic group and diabetic group. Plasma HDL-C was measured by a μl-scale ultracentrifugal method using an RPL-42T rotor (Hitachi Koki Co.). The diabetic hamsters had hypoinsulinemia and hyperlipidemia. Plasma HDL-C in the diabetic group was significantly elevated as compared with the non-diabetic group. A significant positive correlation was observed in both groups between plasma HDL-C and total cholesterol. The male hamsters tended to have higher plasma HDL-C and total cholesterol levels than the female hamsters in either group. Moreover, an electrophoretic analysis showed that there was some relative increase in plasma very low density lipoprotein, low density lipoprotein and high density lipoprotein (HDL) in the diabetic hamsters. An ultracentrifugal analysis showed that plasma chylomicron appeared only in the diabetic hamsters. The heterogeneity of particle size of HDL was found by gradient gel electrophoresis. The apparent average molecular weight of HDL was approximately 265, 000 in either group. It is concluded that plasma HDL-C increased with the advance of hyperglycemia and hyperlipidemia in insulin-deficient diabetic hamsters and that there was sex difference in the hamsters for plasma HDL-C and total cholesterol.View full abstractDownload PDF (1584K) -
HIROSHI MUNAKATA, MAMORU ISEMURA, ZENSAKU YOSIZAWA1984 Volume 144 Issue 3 Pages 291-297
Published: 1984
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSMUNAKATA, H., ISEMURA, M. and YOSIZAWA, Z. Sulfated Proteoglycans Synthesized in Myometrium of the Estrogen-Treated Rabbit. Tohoku J. exp. Med., 1984, 144 (3), 291-297 - Myometrium obtained from the estrogen-treated rabbit was incubated with [35S] sulfate, and the 35S-labeled proteoglycans were extracted from this tissue with 4M guanidine hydrochloride. The extract was fractionated by CsCl density gradient centrifugation, followed by DEAE-cellulose column chromatography. The major proteoglycan fraction (D(1+2)) was separated into two fractions (D(1+2)-I and D(1+2)-II) by gel filtration on Sepharose CL-4B. The compositions of the 35S-labeled glycosaminoglycans in 5 proteoglycan fractions (D(1+2)-I, D(1+2)-II, D3, D4, and D5) were analyzed by two dimensional cellulose acetate membrane electrophoresis after pronase digestion. All the fractions contained the 35S-labeled chondroitin sulfates A/C and heparan sulfate with the different proportions of the radioactivities. The results of the present study indicated that sulfated proteoglycans newly synthesized in myometrium of the estrogen-treated rabbit were considerably heterogeneous not only in the 35S-labeled glycosaminoglycan compositions but also in the molecular weight and buoyant density.View full abstractDownload PDF (1039K) -
HIDEAKI KOHNO, NOBUAKI FURUHASHI, TAKAO FUKAYA, OSAMU SHINKAWA, YOSHIN ...1984 Volume 144 Issue 3 Pages 299-304
Published: 1984
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKOHNO, H., FURUHASHI, N., FUKAYA, T., SHINKAWA, O., TACHIBANA, Y. and SUZUKI, M. Serum Cortisol Levels in Maternal Venous, Umbilical Arterial and Umbilical Venous Blood at Delivery in the Cases of Fetal Distress. Tohoku J. exp. Med., 1984, 144 (3), 299-304 - We measured maternal venous (MV), umbilical arterial (UA) and umbilical venous (UV) cortisol levels in 180 term pregnancies to investigate the changes of these hormone levels in the cases of fetal distress and neonatal asphyxia. The cortisol levels in UA and UV blood in the fetal distress group were significantly higher than those in the non-fetal distress group among spontaneous vaginal delivery cases. There were significant positive correlations among the cortisol levels in MV and UA, MV and UV, and UA and UV blood, respectively. These data suggest that in the case of fetal distress or neonatal asphyxia, mother and fetus have responded independently to the stress of delivery, and that maternal cortisol might not strongly affect the fetal cortisol secretion.View full abstractDownload PDF (533K) -
SHIGEKI VEHARA, CLAUDE A. VILLEE, HIROSHI HOSHIAI1984 Volume 144 Issue 3 Pages 305-313
Published: 1984
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSUEHARA, S., VILLEE, C.A. and HOSHIAI, H. Effect of Estradiol, Prostaglandin E2 and Prostaglandin F2α on Incorporation of [3H] Uridine by Preimplantation Mouse Embryos in Vitro. Tohoku J. exp. Med., 1984, 144 (3), 305-313 - To determine whether estrogen and prostaglandins have a direct effect on the preimplantation embryo, the effect of these compounds added to mouse embryos in vitro was examined. Embryos were incubated in culture media containing [3H] uridine with or without the substances to be tested. The addition of estradiol (10-8M) and prostaglandin F2α (1μg/ml or 10μg/ml) stimulated the incorporation of uridine into RNA. However, prostaglandin E2 at a concentration of 10μg/ml had an inhibitory effect. These findings indicate that estradiol and prostaglandin F2α may have, in addition to an indirect effect mediated by uterine factors, a direct effect on embryonic growth that activates embryos to implant.View full abstractDownload PDF (886K) -
TOYOKI KUGIMIYA, KUNIO SUWA, YUTAKA INADA, KIYOSHI MAYUZUMI, TOSHIO KA ...1984 Volume 144 Issue 3 Pages 315-320
Published: 1984
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKUGIMIYA, T., SUWA, K., INADA, Y., MAYUZUMI, K. and KASAMA, T. Effects of Drug-Induced Reduction in Oxyhemoglobin Affinity on Survival Time of Mice in Severe Hypoxic Conditions. Tohoku J. exp. Med., 1984, 144 (3), 315-320 - We studied the effect of ortho-iodo sodium benzoate (OISB) given intraperitoneally to mice. 300 and 600mg/kg of OISB increased P50 from the control value of 41.2mmHg to 42.7mmHg and 45.7mmHg, respectively. The mice were then exposed to two experimental hypoxias, namely, hypobaric hypoxia and carbon monoxide hypoxia. Mean survival time was prolonged from 67.8 (control saline) to 94.8sec (OISB 300mg/kg) and 112.2sec (OISB 600mg/kg) in the hypobaric hypoxia, and from 201.3sec (control saline) to 329.7sec (OISB 300mg/kg) and 407.7sec (OISB 600mg/kg) in the carbon monoxide hypoxia. We concluded that OISB had prolonged survival time by reducing oxyhemoglobin affinity, thus facilitating oxygen release in severe hypoxic conditions.View full abstractDownload PDF (567K) -
TSUYOSHI YOKOI, KAORU SAGISAKA, MINEO IWASA1984 Volume 144 Issue 3 Pages 321-325
Published: 1984
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSYOKOI, T., SAGISAKA, K. and IWASA, M. Preparation of Anti-Lea and Anti-Rh0 (D) Sera by Immunization with Blood Group Substance Trapped in Autologous Red Cell Ghost. Tohoku J. exp. Med., 1984, 144 (3), 321-325 - Lea substance or immune complex which was prepared with anti-D and D-active Band 3 was trapped in autologous red cell ghost. The trapped immunogens were administered intravenously to rabbits or guinea pigs. Rabbits immunized with Lea substance loading ghost produced antisera of relatively low titers, but they contained specific incomplete anti-Lea antibody (titer 1:128) after absorption procedure, which were higher than that of antisera prepared by the ordinary method. Guinea pig antiserum to the immune complex contained specific incomplete anti-D (titer 1:128). Immunoglobulin class analyses revealed that the anti-Lea consisted mainly of IgM and the anti-D of IgG. It was considered that intravenous injection of the immunogen trapped in ghost is useful for preparing hemagglutinin of sufficient titers.View full abstractDownload PDF (487K) -
MAKOTO UCHIYAMA, TAKESHI OTSUKA, YOSHIHIRO SHIBUY, KAORU SAKAI1984 Volume 144 Issue 3 Pages 327-328
Published: 1984
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSUCHIYAMA, M., OTSUKA, T., SHIBUYA, Y. and SAKAI, K. Abnormal Sodium and Potassium Metabolism in Orthostatic Dysregulation in Childhood. Tohoku J. exp. Med., 1984, 144 (3), 327-328 - Serum sodium (Na) and potassium (K), 12- hr urine Na, K and Na/K ratio and fractional Na excretion rate (FENa) were examined in 142 children with orthostatic dysregulation (O.D.) aged 6 to 15 years. Serum K and FENa were significantly lower in children with O.D. than in normal children, conflicting with the results of low plasma renin activity and aldosterone concentration in children with O.D. which we had previously reported. This suggests that there might be excessive excretion of a mineralocorticoid other than aldosterone or that there might be abnormal regulation of Na and K metabolism by the renin-angiotensin-aldosterone system.View full abstractDownload PDF (192K) -
HIROSHI SUZUKI, TASUKE KONNO, SETSUKO KITAOKA, TETSUO SATO, AKI IMAI, ...1984 Volume 144 Issue 3 Pages 329-330
Published: 1984
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSUZUKI, H., KONNO, T., KITAOKA, S., SATO, T., IMAI, A., TAZAWA, F. and ISHIDA, N. “Enveloped” Rotavirus Particles Detected in Culture Fluid. Tohoku J. exp. Med., 1984, 144 (3), - 329-330 In a negative contrast preparation of human rotavirus culture fluid were found membrane-bound “enveloped” virus particles which had been observed only in thin sections of the infected cells. The “enveloped” particles comprised a single-shelled particle and degenerating membrane structure.View full abstractDownload PDF (1441K)
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