MATSUE, Y., WAGATSUMA, S., ITO, K. and OKUMA, T. Smooth PursuitEye Movements in Epileptics under Antiepileptic Medication. Tohoku J. exp. Med., 1981, 134 (4), 341-349 1) Smooth pursuit eye movements (SPEM) were recorded in 18 normal, 20 schizophrenic and 50 epileptic subjects. Most of the schizophrenic and epileptic subjects were under medication, antipsychotic drugs in the former and antiepileptic drugs in the latter. 2) In schizophrenics and epileptics, rate of the saccadic component in SPEM was higher than that of normal controls. The rate of large amplitude saccades was higher in epileptics than in schizophrenics. 3) Though small amplitude saccades were usually superimposed on SPEM in schizophrenic patients, both large and small amplitude saccades superimposed irregularly on the smooth tracking curve were observed in epileptics, and overshoots or undershoots were also seen frequently. 4) SPEM disorder in epileptics was observed in more than half of patients receiving antiepileptic medication, especially in patients with a long period of treatment. SPEM disorder in epileptics tended to occur frequently in patients who received phenytoin in doses higher than a certain level; i.e. approximately 150mg per day for a long period of time. 5) It is conceivable that the SPEM disorder represents a latent or subclinical cerebellar dysfunction due to phenytoin medication. It is suggested that the SPEM measurement is a useful tool to find out subclinical side effects of antiepileptic drug in the treatment of epilepsy.
WAKIZAKA, A., KUROSAKA, K., OKUHARA, E., KASHIMURA, M., AKIHAMA, T., MIURA, A.B. and SHIBATA, A. The Non-Specific Binding of Thermally DenaturedDNA by Healthy Human Sera in Farr Assay with or without Addition of DextranSulfate and Calcium Chloride. Tohoku J. exp. Med., 1981, 134 (4), 351-356-A non-specific DNA binding by human sera was measured in 60 male and female healthy subjects in different ages. Farr assay using thermally denatured mouse embryo [3H]DNA proved a high DNA binding level of 47.9±5.8% (mean ±S.D.) on an average of these sera. The addition of dextran sulfate and CaCl2 significantly decreased the mean binding value to 14.7±4.8% and 6.5±3.2%, respectively. No significant difference was observed between men and wemen, or between any age groups from twenties to sixties. The measurement of complement activity in the serum gave no evidence for the participation of complement in the non-specific DNA binding.
KASHIMURA, M., WAKIZAKA, A., KUROSAKA, K., OKUHARA, E., AKIHAMA, T., MIURA, A.B. and SHIBATA, A. Differentiation of Specific DNA Binding Activity ofSLE Sera from Non-Specific Binding by an Addition of Dextran Sulfate and CalciumChloride to the Farr Assay System. Tohoku J. exp. Med., 1981, 134 (4), 357-365-A new method for differentiation of specific DNA-binding by human sera from non-specific binding was evaluated with sera from patients with systemic lupus erythematosus in different stages of the disease. An addition of dextran sulfate or clacium chloride to Farr's radioimmunoassay mixture reduced non-specific binding of thermally denatured [3H]DNA of the patient sera without much effect on the specific binding. The measurement of the DNA-binding value by the sera in these addition systems provides accurate information with regard to the pathophysiological state of the disease.
MUKADA, T., ANDOH, N. and SASANO, N. Tumor of the Testis: An Attemptof Histological Classification According to WHO Guide Line. Tohoku J. exp. Med., 1981, 134 (4), 367-373 -Testicular tumor in Japan is one of the evil neoplasms which have increased in incidence rate in recent years. In order to provide the basic data for the clinicopathological and epidemiological studies on testicular tumors, the WHO histological classification was applied to 86 cases in the file of Department of Pathology, Tohoku University School of Medicine, in the years of 1969 through 1980. Of 86 cases, 75 (87%) were germ cell tumors including 37 seminomas, 10 yolk sac tumors, 4 embryonal carcinomas, 9 mature teratomas, and 15 combined forms. The combined forms consisted mainly of embryonal carcinoma and immature teratoma. Malignant lymphoma was encountered in 8 cases. These malignant tumors showed characteristic age preponderance. Non-germ cell benign tumors were recorded in only 3 cases. Briefly mentioned were some histological diagnostic hallmarks of malignant testicular tumors.
SATO, N., KAGAWA, Y., NITTA, S., MOHRI, H. and HORIUCHI, T. Evaluationof Mechanical Left Ventricular Assistance with Left Atrium to Aorta Bypass inDogs. Tohoku J. exp. Med., 1981, 134 (4), 375-384 Effects of the left heart bypass (LHB) by the left atrium (LA) to the aorta (AO) shunt on the heart were evaluated by measuring such parameters as tension time index (TTI), diastolic pressure time index (DPTI), DPTI/TTI ratio, coronary blood flow and myocardial oxygen consumption (MVO2) in open-chest dogs. A pneumatically driven bypass pump made of polyurethane was utilized in this study. No significant change of MVO2 was observed with surgical maneuver to connect the LA-AO LHB pump to experimental animals. During synchronous pumping of LHB, TTI decreased by 35%, MVO2 decreased by 56% of the control and DPTI/TTI ratio increased by 79%. However, coronary flow decreased by 35%, presumably due to autoregulation mechanism of the coronary circulatory system. Both synchronous and asynchronous LHB demonstrated the same level of effect in volume unloading. However, significant diastolic augmentation was noted only in the former. LA-AO LHB, which has a sufficient supporting effect and does not damage the ventricular wall, was considered to be very useful for temporary support for the failing heart, especially for postoperative low output state.
ABE, I., HORI, K., SAITO, S., SUZUKI, M. and SATO, H. Effect of AttenuatedCells on the Growth of Small Implant of Yoshida Ascites Hepatoma AH109A. Tohoku J. exp. Med., 1981, 134 (4), 385-392 -The transplantability of small implants such as 10 cells of Yoshida ascites hepatoma AH109A, which grew lethally only in 2 out of 19 rats when transplanted subcutaneously, was improved by admixed implantation of attenuated tumor cells prepared with mitomycin C, presenting lethal take in 23 out of 27 animals. Similar effect was also obtained by liver homogenate or sonicated tumor cell suspension. Although the rate of tumor “take” of less than 103 cells of AH109A inoculated in the subcutis increased by admixing the attenuated cells, growth characteristics of such a small implant of the tumor, once initiated, were not significantly different whether inoculated with or without the attenuated cells. Growth support effect of attenuated cells was discussed in relation with either the size of inoculum or growth ability of tumor cells.
HANEDA, T., NIWA, T., MIURA, T., MIYAZAWA, K. and TAKISHIMA, T. Echocardiographic and Morphological Features of the Pulmonic Valve in Patientswith Primary Pulmonary Hypertension and Those with Secondary PulmonaryHypertension Resulting from Left-to-Right Shunt. Tohoku J. exp. Med., 1981, 134 (4), 393-400 -In 11 patients with primary pulmonary hypertension (PPH) and 16 with secondary pulmonary hypertension due to left-to-right shunt (SPH), ehocardiographic and morphological features of the pulmonic valve were studied. Mean ages of these patients were 27 and 31 years, respectively. The echo intensity of the pulmonic leaflet was graded as either “strong” or “not strong” by comparison with the adjacent tissue. In the PPH group, the valve echo was “not strong”, while it was “strong” in the majority of the cases of SPH. In autopsy, there were no morphological changes in the pulmonic valves in the former, while these valves were definitely hypertrophic in the latter. The average thicknesses of the valve edges in the two groups of patients were 0.5 and 1.6mm, respectively. The results suggest that the different echo intensities reflect the morphological differences of the pulmonic valve; and this provides a diagnostic value for differentiating the two conditions.
KUMANO, N., KIKUMOTO, T., KOINUMARU, S., NAKAI, Y., ISHIKAWA, T., SUZUKI, S. and KONNO, K. Prophylaxis of 3-Methylcholanthrene-InducedTumorigenesis in Mice with the Yeast Polysaccharide Preparation. Tohoku J. exp. Med., 1981, 134 (4), 401-409 -Prophylaxis of 3-methylcholanthrene-induced epithelial tumor formation in ddI mice with our Candida utilis glucomannan preparation (YPS) was confirmed further in the present system of fibrosarcoma production. A single subcutaneous dose of MCA (0.5mg per mouse) was either preceded by or followed by 10 or 30 daily intraperitoneal (i.p.) injections of YPS at the dosage of 100mg/kg per dose according to various time schedules. Protective effect of YPS was demonstrable by a significantly lengthened latent period before the tumor appearance and a slower rate of the subsequent tumor production until around day 100. All the schedules of pre- or post-treatment were found to be effective, although the one delayed until two weeks post MCA was so only marginally. It was also disclosed by prolonging the observation period that this protection was of a transient nature, 100 per cent tumor incidence being found by around 150 days post MCA in all the experimental groups. A longer latency due to the treatment with YPS was generally found in association with a faster growth of the tumor and a shorter life span of the host animal. Temporary protection of MCA-induced tumorigenesis in ddI mcie was not reproducible in C3H/He mice, and was thus suggested the possible strain-dependent nature of YPS effect.
YASUDA, K., SAITO, S., YAMAMOTO, M., OKUYAMA, M., YOSHINAGA, K, and MIURA, K. Carbohydrate Metabolism in Subacute Thyroiditis: Effect of AcuteElevation of Thyroid Hormones. Tohoku J. exp. Med., 1981, 134 (4), 411-416-Carbohydrate metabolism was studied in nine patients with Subacute thyroiditis before treatment and after recovery. The mean fasting free fatty acid level before treatment was significantly higher than that after the recovery, although the mean fasting blood glucose and insulin levels were not significantly different. Seven out of nine patients showed glucose intolerances. They returned to normal after the recovery except two cases with family histories of diabetes mellitus, whose glucose intolerances remained even 6 months after the recovery. Glucose area during oral glucose tolerance test was significantly correlated with the elevated thyroxine (r=0.8, p<0.01), and triidothyronine (r=0.66, p<0.05). The results indicated the importance of follow-up study of glucose tolerance in subacute thyroiditis as well as similarity of carbohydrate metabolism abnormalities in hyperthyroidism.
ASAKAWA, H., OTAWA, H., YAMADA, S. and MATSUMOTO, K. CombinationTherapy of Esophageal Carcinoma with Radiation and Bleomycin. Tohoku J. exp. Med., 1981, 134 (4), 417-424-The concurrent combination therapy of radiation and Bleomycin was performed in a total of 175 cases of esophageal carcinoma. In 107 (63%) cases of them, in which the scheduled curative treatment was completed, yearly survival rates were 60% in one, 30% in two, 20% in three and 7% in five years. The local recurrence was noted in 60% and remote metastases were observed in 38% of them. These results were intimately correlated to tumor advancement and cancer type. From the results, it was concluded that this combination therapy should be valuable to prolong the life of a cancer patient, improving one to three year survival rates, and that the curable indications for this treatment should be T1 M0 and T2 M0 with tumorous or some ulcerous types of carcinoma.
TORINUKI, W., KUMAI, N. and MIURA, T. Histopathological Studies on Sun-Exposed Hexachlorobenzene-Induced Porphyric Rat Skin. Tohoku J. exp. Med., 1981, 134 (4), 425-430-Hexachlorobenzene (HCB) is a porphyrogenic agent. The inducement of skin changes was attempted through repeated exposure of the skin of HCB-induced porphyric rats to sunlight. The following skin changes were produced in the porphyric rats; erythema, erosion, crust, skin thickening and scarring. Histopathological examination revealed the presence of acanthosis, vacuolization of the malpighian cells, subepidermal vesicle, fibrosis, dilatation and increase of the blood vessels and perivascular cell infiltration composed of lymphocytes, histiocytes and mast cells. The PAS stainability of blood vessel walls was slightly intensified. The assumption is that photosensitive flares were elicited within the short 2-month period though destruction of endothelial cells was not prominent. There were no distinct skin changes, clinically or histopathologically, in any of the three control groups.
KOBAYASHI, Y., WATANABE, H., SUZUKI, H., KONNO, T. and YAMAMOTO, T.Y. Ultrastructural Studies on Congenital Generalized Fibromatosis RegressedSpontaneously. Tohoku J. exp. Med., 1981, 134 (4), 431-445-Cellular components of a subcutaneous nodule from a male infant with congenital generalized fibromatosis consisted of myofibroblasts, fibroblasts, and their various intermediate types. The myofibroblasts were characterized by the presence of bundles of microfilaments and dense bodies similar to those in smooth muscle cells. Membrane-bound vesicles containing dense material were abundant in the extracellular space. They were probably lysosomes released by disintegration of cellular components. A possible role of the myofibroblasts and extracellular lysosomes in the regression of nodular lesions was discussed.