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Volume 169 (1993)
- Issue 4 Pages 261-
- Issue 3 Pages 179-
- Issue 2 Pages 91-
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Volume 169, Issue 1
Displaying 1-9 of 9 articles from this issue
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YASUO FURUYA, TAKURO OGATA1993 Volume 169 Issue 1 Pages 1-19
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSFURUYA, Y. and OGATA, T. Scanning Electron Microscopic Study of the Collagen Networks of the Normal Mucosa, Hyperplastic Polyps, Tubular Adenoma, and Adenocarcinoma of the Human Large Intestine. Tohoku J. Exp. Med., 1993, 169(1), 1-19-The three-dimensional structure of the collagen network of normal mucosa, hyperplastic polyps, tubular adenoma, villous adenoma and adenocarcinoma of the human large intestine was examined by scanning electron microscopy after cell-maceration with a low temperature NaOH solution. In the normal large intestine, the surface of the sub-basal laminar collagen sheath lining the crypts was generally smooth. In hyperplastic polyps, the crypts extended and the sub-basal collagen network protruded from the surrounding normal tissue. The sub-basal laminar collagen sheath was more densely arranged near the openings of the crypts and on the luminal surface than in deeper regions of the crypts. In tubular adenoma, the tumor glands showed meandering, bending, and divisions. The sub-basal laminar collagen sheath was composed of densely packed collagen fibrils. In villous adenoma, the sub-basal laminar collagen sheath formed foliate or linguiform villous cores projecting sharply into the lumen. In adenocarcinoma, the sub-basal laminar collagen sheath exhibited a dense arrangement of fibrils in the central region of the tumor. Toward the marginal region, the collagen sheaths surrounding tumor glands became thinner, and at the edge of the marginal region, basket-like collagen sheaths were observed around individual tumor cells infiltrating the interstitium. Such a tumor lesion was frequently surrounded by a thick collagen fiber zone.-large intestine; intestinal polyps; intestinal cancer; collagen fiber; scanning electron microscopyView full abstractDownload PDF (10732K) -
TAMIO WAKUGAMI, MOHAMMAD RASHIDUL ISLAM, SEIKEN HIGA, KENJI MURAKAMI, ...1993 Volume 169 Issue 1 Pages 21-29
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSWAKUGAMI, T., ISLAM, M.R., HIGA, S., MURAKAMI, K, and MIMURA, G. Effect of FK506 on the Development of Diabetes in BB Rats in Comparison with That of Cyclosporin. Tohoku J. Exp. Med., 1993, 169(1), 21-29-FK506, a agent extracted from a streptomyces, has more potent immunosuppressive properties compared with cyclosporin in vitro. We compared the preventive effect of FK506 on the development of diabetes mellitus with that of cyclosporin in BB rats, which are regarded as a useful model of insulin-dependent diabetes mellitus. BB rats aged 30 days were treated intramuscularly with FK506 (0.1mg/kg/day and 0.32 mg/kg/day) or with cyclosporin (10mg/kg, alternate days) until 150 days of age. Diabetes developed in 2 (10.5%) of 19 rats treated with the lower dose of FK506 and none of 20 rats with the higher dose of FK506; on the other hand, 1 (5.3%) of 19 rats treated with cyclosporin developed diabetes. In contrast, 9 (36.0%) of 25 control rats became diabetic. The cumulative incidence of diabetes mellitus in the group treated with FK506 (0.32mg/kg) showed a decrease similar to or more than that of the cyclosporin-treated group. Histological examination showed that FK506 and cyclosporin prevented the reduction in the average size of islets and in the area of beta cells. The analysis of lymphocyte subsets proved the decrease of W3/25: OX8 ratio in both FK506- and cyclosporin-treated groups. These data suggest that the administration of FK506 might be a more useful tool for preventing the development of insulin-dependent diabetes mellitus.-FK506; insulin dependent diabetes mellitus; immunotherapy; BB ratsView full abstractDownload PDF (1176K) -
MASAHITO SAKUMA, KUNIO SHIRATO, YOSHIOHIKA OIKAWA, ATSUSHI KATOH, MAKO ...1993 Volume 169 Issue 1 Pages 31-41
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSAKUMA, M., SHIRATO, K., OIKAWA, Y., KATOH, A., NAKAGAWA, M., ISHIGAKI, H. and TAKISHIMA, T. Effects of Changes in Afterload on Regional Wall Motion in Acute Ischemic Canine Heart. Tohoku J. Exp. Med., 1993, 169 (1), 31-41- The aim of the present study was to examine effects of changes in afterload on regional myocardial motion in acute ischemia. Ischemic and non-ischemic segment lengths of the left ventricular free wall were measured by miniature ultrasonic gauges in eleven open chest dogs with the pericardium preserved. In a stable state after left anterior descending coronary artery occlusion, peak left ventricular pressure was varied by the infusion of angiotensin II (n=8) and sodium nitroprusside (n=8). To exclude effects of preload on the responses, end-diastolic lengths of the non-ischemic region before and during infusion of each drug were matched with vena caval occlusion. When peak left ventricular pressure elevated from 113±2 (mean±S.E.)mmHg to 145±6, in isovolumetric contraction phase, degree of active shortening in the non-ischemic region and that of paradoxical expansion of the ischemic region did not change. In ejection phase, active shortening of the non-ischemic region decreased from 1.38±0.11mm to 1.06±0.10 but that of the ischemic region remained unchanged. Stroke volume decreased from 14.5± 1.3ml to 10.8±1.0. When peak left ventricular pressure decreased from 111±4 mmHg to 101±6, in isovolumetric contraction phase, active shortening of the non- ischemic region decreased from 0.90±0.13mm to 0.76±0.15 and paradoxical expansion of the ischemic region reduced from -0.95±0.11mm to -0.80±0.11. In ejection phase, shortening of the non-ischemic region increased from 1.05±0.13 mm to 1.31±0.15 but that of the ischemic region did not change. Stroke volume increased from 11.5±1.3ml to 14.0±1.4. These results indicate that in acute ischemia, changes in isovolumetric shortening of the non-ischemic region and paradoxical expansion of ischemic region are related with each other in isovolumetric contraction phase when afterload is altered and suggest that stroke volume is affected by the shortening of ejection phase in the non-ischemic region. -angiotensin II; sodium nitroprusside; pericardiumView full abstractDownload PDF (1201K) -
MINORU YASUJIMA, MASAYUKI KANAZAWA, KAZUNORI YOSHIDA, MASAHIRO KOHZUKI ...1993 Volume 169 Issue 1 Pages 43-50
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSYASUJIMA, M., KANAZAWA, M., YOSHIDA, K., KOHZUKI, M., WATANABE, H., HIWATARI, M., SATO, T., ABE, K., HIRATA, Y. and YOSHINAGA, K. Chronic Treatment with Anti-Endothelin Antibodies Fails to Modify the Development of Hypertension in Stroke-Prone Spontaneously Hypertensive Rats and DOCA-Salt Hypertensive Rats. Tohoku J. Exp. Med., 1993, 169 (1), 43-50-This study was designed to assess whether blocking endogenous endothelin with anti- endothelin antibodies could alter the development of hypertension in stroke-prone spontaneously hypertensive rats (SHR) and DOCA-salt treated rats. Specific anti-endothelin antibodies were produced in rabbits by standard methods. The amount of anti-endothelin antibodies employed in this study blocked the hypertensive effect of endothelin-1, 750ng/kg, by 55% in conscious rats. Intravenous injection of anti-endothelin antibodies as a bolus twice a week for 3 weeks did not affect the rise in blood pressure of stroke-prone SHR (268±8mmHg, n=8) compared to control stroke-prone SHR (256±7mmHg, n=8) treated with normal rabbit serum. Intravenous administration of anti-endothelin antibodies in a same manner also failed to alter the development of hypertension in DOCA-salt treated rats (160±6mmHg in anti-endothelin antibodies-treated group, n=7 compared to 164±5mmHg in normal rabbit serum-treated group, n=7). The administration of anti-endothelin antibodies did not induce any significant changes in body weight, urine volume and urinary sodium excretion in stroke-prone SHR and DOCA-salt treated rats compared to those treated with normal rabbit serum. These findings suggest that circulating endothelin might not play a major role in the regulation of blood pressure in stroke-prone SHR and DOCA-salt treated rats. -endothelin; anti-endothelin antibodies; stroke-prone SHR; DOCA-salt hypertensive rats; hypertensionView full abstractDownload PDF (780K) -
YOSHIAKI KONDO, YUTAKA IGARASHI, KEISHI ABE, KEIYA TADA1993 Volume 169 Issue 1 Pages 51-58
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKONDO, Y., IGARASHI, Y., ABE, K. and TADA, K. New Double-Barreled, Ion-Sensitive Microelectrodes for Measuring Intracellular Cl- Activities in Rabbit Renal Collecting Ducts. Tohoku J. Exp. Med., 1993, 169 (1), 51-58-The use of the conventional ion-sensitive double-barreled microelectrodes has been unsuccessful for studying small epithelial cells such as those of the collecting duct because of the difficulty in obtaining the ideal electrodes for impaling cells. We developed a new type of ion-sensitive, double-barreled microelectrode of a size and tip configuration ideal for use in impaling small cells such as the principal cells (PCs) of the cortical collecting ducts, and remaining therein, for more than 30min. The electrodes were pulled in two steps. The first includes the reciprocal twisting of two parallel glass capillaries, without pulling, to form a round, nontortuous fused glass capillary in the puller. In the second step, the round, fused straight capillaries are pulled so as to form the tip. This resulting procedure enabled us to impale the PCs without altering their cell membrane potentials. The basolateral membrane voltage (Vb) in PCs of the cortical collecting ducts was -69.1±1.6mV (n=8) and intracellular Cl- activity ([Cl]1) in PCs of the cortical collecting duct was 11.8±0.9mmol/liter (n=5). Changing the concentration of Cl- in the ambient solution showed that the basolateral cell membrane of the PCs was highly permeable to Cl-. This new electrode will help to obtain new information on intracellular ion handling, with the electrodes applied to the collecting duct cells. -chloride transport; ion exchanger; renal tubule; electrophysiology; aluminosilicate glassView full abstractDownload PDF (848K) -
HIROKO KODAMA, YUKO MEGURO, AKIKO TSUNAKAWA, YUTAKA NAKAZATO, TOSHIAKI ...1993 Volume 169 Issue 1 Pages 59-66
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKODAMA, H., MEGURO, Y., TSUNAKAWA, A., NAKAZATO, Y., ABE, T. and MURAKITA, H. Fate of Orally Administered Triethylenetetramine Dihydrochloride: A Therapeutic Drug for Wilson's Disease. Tohoku J. Exp. Med., 1993, 169 (1), 59- 66-Triethylenetetramine dihydrochloride (TETA) is a therapeutic drug for Wilson's disease. We developed a simple fluorometric method for detection of TETA in biological fluids by using high-performance liquid chromatography (HPLC), and examined TETA concentrations in the serum and urine of two healthy adults who were given TETA orally. No TETA peak was detected in the serum. The amount of TETA in the urine of the two adults was only 1.6 and 1.7% of the dose administered. However, a large unidentified peak appeared in the urine after oral administration. This peak was not observed in a mixture of TETA and control urine or in urine before TETA administration. When the urine after TETA administration was analyzed after hydrolysis with HCl, the unidentified peak disappeared, while the TETA peak increased. These findings indicate that the substance which yielded the unidentified peak is a metabolite of TETA, suggesting that most of the TETA administered is metabolized and then excreted in the urine.-triethylenetetramine dihydrochloride; Wilson's disease; analytical method; urine; TETAView full abstractDownload PDF (711K) -
KIYOSUMI OHISHI, KUNIHIKO NAGASATO, WATARU AOI, TATSUFUMI NAKAMURA, KA ...1993 Volume 169 Issue 1 Pages 67-75
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSOHISHI, K., NAGASATO, K., AOI, W., NAKAMURA, T., ICHINOSE, K., NISHIURA, Y., SATOH, A., TSUJIHATA, M., SHIBATA, Y. and NAGATAKI, S. Circadian Rhythms of Blood Pressure and Heart Rate in Patients with Human T-Lymphotropic Virus Type-I Associated Myelopathy. Tohoku J. Exp. Med., 1993, 169 (1), 67-75 - Noninvasive ambulatory blood pressure and heart rate monitoring were used to investigate cardiovascular dysfunction in patients with human T-lymphotropic virus type-I(HTLV-I)-associated myelopathy (HAM). The subjects were 23 patients with HAM, and 23 sex- and age-matched normotensive healthy volunteers (controls). Circadian rhythms of blood pressure and heart rate were present in both the HAM patients and controls. Amplitudes and the 24-hr mean systolic and diastolic blood pressures were significantly lower in the patients than in the controls. The 24hr mean heart rate was significantly higher in the patients, the difference being particularly marked during the night. Differences in the acrophases of the systolic and diastolic blood pressures and heart rate between patients and controls were small but still significant. These results suggest that subclinical cardiovascular autonomic dysfunction is present in HAM patients. - human T-lymphotropic virus type-I-associated myelopathy (HAM); ambulatory blood pressure monitoring; circadian rhythm; cardiovascular autonomic dysfunctionView full abstractDownload PDF (882K) -
MAKIKO SETO, MASAKATSU MOTOMURA, GOU TAKEO, TOSHIRO YOSHIMURA, MITSUHI ...1993 Volume 169 Issue 1 Pages 77-86
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSETO, M., MOTOMURA, M., TAKEO, G., YOSHIMURA, T., TSUJIHATA, M. and NAGATAKI, S. Treatment of Myasthenia Gravis: A Comparison of the Natural Course and Current Therapies. Tohoku J. Exp. Med., 1993, 169 (1), 77-86 - Ninety-five patients with generalized myasthenia gravis were followed for 10 years to evaluate the long term effects of prednisolone, thymectomy, or both, and they were compared with a group only with anticholinesterase treatment. Only 15.0% of the patients with anticholinesterase alone had showed improvement 10 years after the onset, but more than 60% of those treated with prednisolone, thymectomy, or both showed improvement. Especially thymectomy induced complete remissions at 10 years after surgery in more than 20% of the patients. There was no difference between the histology of the thymi and clinical severity, or anti-acetylcholine receptor (AChR) antibody titer. In patients who showed improvement classified “good” of higher 10 years after thymectomy with or without prednisolone, anti-AChR antibody titers swiftly decreased to 37.8% of the value before surgery, and remained low thereafter. This result suggests that the marked decrease in anti-AChR antibody titers within 1 month after thymectomy is a favorable prognostic sign in myasthenia gravis patients who have undergone thymectomy. - myasthenia gravis; prognosis; anti-AChR antibodies; thymectomy; prednisoloneView full abstractDownload PDF (1113K) -
HARUO NAGASAWA, KYUYA KOGURE, TATSUO IDO1993 Volume 169 Issue 1 Pages 87-89
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSNAGASAWA, H., KOGURE, K. and IDO, T. Simultaneous Demonstration of Neurotransmitter and Receptor Systems of the Rat Brain Using In Vivo Double Autoradiography. Tohoku J. Exp. Med., 1993, 169 (1), 87-89 -Neurotransmitter and receptor systems of the rat brain were simultaneously observed by in vivo double autoradiography using a novel imaging plate analyzer system which was developed by Fuji Photo Film Co., Ltd. The animals were subjected to intravenous administration of [18F]-6-fluoro-L-dopa ([18F]-DOPA) and [3H]YM- 09151-2, a highly selective dopamine D2 antagonist. The radioactivities of [18F]-DOPA and [3H]YM-09151-2 were found to be highly concentrated in the striatum, demonstrating presynaptic and postsynaptic sites of the dopaminergic nigrostriatal tract, respectively. The [3H]YM-09151-2 was also found to be accumulated in the substantia nigra which is known to correspond with the distribution of dopamine D2 receptors. This method was shown to be useful for investigation of animal models with brain diseases. - neurotransmitter; receptor; double autoradiography; [18F]-DOPA; [3H]YM-09151-2View full abstractDownload PDF (577K)
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