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Volume 107 (1972)
- Issue 4 Pages 303-
- Issue 3 Pages 203-
- Issue 2 Pages 105-
- Issue 1 Pages 1-
Volume 107, Issue 3
Displaying 1-9 of 9 articles from this issue
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MAKOTO SEIJI, YOSHIO TANAHASHI, ATSUSHI OIKAWA1972 Volume 107 Issue 3 Pages 203-216
Published: 1972
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe relationship between cell proliferation and differentiation was studied using C341 cells' isolated from a culture cell line of mouse melanoma B-16. The C341 cells' rate of melanin formation is intimately related to active cell proliferation. The cells were examined with light and electron microscopes on the 4 th day of cultivation when the cells were proliferating exponentially and on the 7 th day when the culture was in the stationary phase. The 4-day-old culture consisted of dark, light and melanosome-containing cells. Melanosomes, mostly in the early stages of development, were found to be present in the light cells as well as in the melanosome-containing cells. In cells of the 7 th day culture, there were numerous melanosomes in various developmental stages and also melanosome complexes. The morphological evidence obtained is compatible with the biochemical evidence previously recorded.View full abstractDownload PDF (6398K) -
FUMIMARO TAKAKU, YUZO YANO, YOSUKE AOKI, KIKU NAKAO, OSAMU WADA1972 Volume 107 Issue 3 Pages 217-228
Published: 1972
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSA method to measure the δ-aminolevulinic acid (ALA) synthetase activity in human bonemarrow erythroid cells by incubating the bone-marrow cell homogenate with 14Ca-ketoglutarate has been presented. Studies representing an attempt to obtain the optimal conditions for the enzyme assay and to confirm the reliability of the results of the assay were performed. ALA-synthetase activity in erythroblasts was found decreased in all 4 cases of iron deficiency anemia. In the cases of sideroblastic anemia, the enzyme activity was markedly decreased to less than one-tenth of normal in one case, moderately decreased in 2 cases, and normal in 2 cases. In a case with β-thalassemia, a marked decrease in ALA-synthetase activity was in evidence. In these cases of the sideroblastic anemia or thalassemia with markedly decreased ALA-synthetase activity, a substantial diminution of 14C-glycine incorporation into heme in erythroblasts was also evident. Erythroblasts cf these cases, however, showed only a slight decrease in the heme-synthetase activity. In erythroblasts of patients with erythropoietic protoporphyria, ALA-synthetase activity was found to be within normal limits in 2 cases, and apparently increased in one case.View full abstractDownload PDF (664K) -
HIROSHI OHTA, ELLIOTT F. OSSERMAN1972 Volume 107 Issue 3 Pages 229-240
Published: 1972
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSIn order to elucidate the cause of markedly increased serum and urine lysozyme (muramidase) in patients with monocytic leukemia on the cellular basis, monocytes and neutrophils were compared on their hydrolytic enzyme contents and their characteristics. Both types of leukocytes were separated from normal human blood or from patients with monocytic leukemia by means of floatation in dense albumin, utilizing the difference in the specific gravity of both cell types. Equivalent lysozyme and acid phosphatase activities were found to be present in monocytes and neutrophils and very low or negligible activities of alkaline phosphatase in monocytes. The subcellular distributions of these enzymes were comparable in the pattern and specific activity in both cell populations and found to be associated with granules. The phagocytic rate and the readiness of release of lysozyme into the extracellular medium during phagocytosis were similar in both cell populations. Leukemic leukocytes, neutrophils and monocytes, showed lower hydrolase activities than normal counterparts, especially alkaline phosphatase being markedly suppressed in leukemic cells. The possibility was discussed that the mechanism for elevated lysozyme in serum and urine of patients with monocytic leukemia may be accounted for by the developmental potential of monocytes in extravascular sites into macrophages with marked increase in lysosomal enzymes, the relatively ready release of lysozyme from cells, and a stable nature of lysozyme.View full abstractDownload PDF (725K) -
AKIRA OHNEDA, MUNEHIKO SATO, KIYOSHI MATSUDA, AKIRA YANBE, YOSHISUKE M ...1972 Volume 107 Issue 3 Pages 241-251
Published: 1972
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe response of plasma glucagon to several stimuli was studied and compared in normal and diabetic subjects. Plasma glucagon was measured by the radioimmunoassay using an anti-glucagon serum, which is relatively specific for pancreatic glucagon. Insulin-induced hypoglycemia caused a marked elevation of plasma glucagon in normal subjects but minimal in diabetic patients. A rapid fall of blood glucose, cataglycemia, induced by the discontinuation of glucose infusion did not show any changes in the level of plasma glucagon. The response of plasma glucagon was not observed after the injection of tetrapeptide of gastrin or secretin, although these hormones induced an increase in the level of plasma insulin. Pancreozymin did not induce any changes in plasma insulin or glucagon in a dosis of one clinical unit per kg of body weight. The infusion of 30g of L-arginine stimulated the secretion of pancreatic glucagon and the plasma level of glucagon was moderately elevated in both normal and diabetic subjects. These results suggest that L-arginine infusion is recommended as a tool for the alpha-cell function test of the pancreas in man.View full abstractDownload PDF (655K) -
ISAMU USUBUCHI, YUKIO SOBAJIMA, HAJIME KUDO, MASAFUMI KANO, TATSUSUKE ...1972 Volume 107 Issue 3 Pages 253-262
Published: 1972
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSHost resistance against the transplantation of allogeneic tumors, such as Yoshida sarcoma, Hirosaki sarcoma, Takeda sarcoma or Ascites Hepatoma 7974, occurs in rats immunized with allogeneic Usubuchi sarcoma. It is considered that allogeneic tumors of rats have common antigens and this antigenicity causes cross-immunity among various allogeneic tumors. The cross-immunity induced by the common antigens was usually weak as compared with the immunity demonstrated in the re-transplantation of the same tumor. In such a state, the antigenicity peculiar to the host, in which each tumor originated, acts effectively in addition to the common antigens.View full abstractDownload PDF (555K) -
KOROKU HASHIMOTO, TOSHIHIKO IIJIMA, KEITARO HASHIMOTO, NORIO TAIRA1972 Volume 107 Issue 3 Pages 263-275
Published: 1972
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe isolated AV node preparation of the dog consists of the right atrium and ventricular septum, and thus includes the main conducting pathways from the sinoatrial node through peripheral Purkinje fibers. The preparation is cross-circulated with a donor dog through the cannulated right coronary, anterior septal (ASA), and posterior septal arteries (PSA). The preparation permits close-arterial injection of drugs into the AV nodal area selectively via either the PSA or the ASA. The preparation also permits direct visual placement of recording electrodes at desired sites along the main conducting system. The preparation runs for 12 hr or more. Relation of AV conduction to rates of atrial pacing observed in this preparation is essentially the same as that observed in the isolated hearts. Occlusion of the PSA causes impairment of AV conduction, while that of the ASA causes only diminution of ventricular potentials. Acetylcholine causes a negative dromotropic effect and l-norepinephrine a positive one via both the PSA and the ASA. However, the two substances are more effective via the PSA than via the ASA.View full abstractDownload PDF (761K) -
YUKIMASA HIRATA, HIROSHI ISHIZU1972 Volume 107 Issue 3 Pages 277-286
Published: 1972
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThis report deals with a Japanese male patient who showed severe spontaneous hypoglycemic attacks and mild diabetes. When he was 47-year-old, the first attack of unconsciousness occurred, and after that he was suffering from the same attacks for 5 years. After 3 years from the first attack mild diabetes was found. He was admitted in June, 1968, when he was 52-year-old. Although the patient had never been treated with insulin, his serum showed insulin-binding capacity over 3 years under our observation. A large amount of insulin determined as immunoreactive insulin was extracted with acid ethanol from his native serum. A binding reaction between the insulin-free gamma globulin fraction of the serum and pig 131I-insulin was more inhibited by crystalline human insulin than by crystalline beef insulin. No insuloma was found, but one-third of the pancreas was excised on August 21, 1968. In the histological examination a hyperplasia of the pancreatic islets was significant and there was no inflammatory reactions in the islets. After the operation the hypoglycemic attacks ceased, but the insulin-binding capacity of the serum and mild diabetic state were still observed in July, 1971.View full abstractDownload PDF (1157K) -
KOEI FUJII, SHOICHI YAMAGATA, JINICHI SUZUKI, RIKURO SASAKI, TAKEHIKO ...1972 Volume 107 Issue 3 Pages 287-299
Published: 1972
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSSelective celiac arteriography was performed on 18 patients with various submucosal tumors of the stomach, diagnoses of which were confirmed by surgical operation except for one case. Angiographic findings were reviewed in comparison with macroscopic and histologic findings of operative materials. It was found that there were characteristic angiographic features depending upon the sort of tumors. It is considered that this method is useful to determine the localization and the extent of the tumor, and is valuable to differentiate benign from malignant lesion.View full abstractDownload PDF (5834K) -
SHINOBU WATANABE1972 Volume 107 Issue 3 Pages 301-302
Published: 1972
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSA simple and rapid method was presented for qualitative and quantitative analysis of organophosphate pesticides in human blood serum, which employs a gas chromatograph fitted with flame thermionic detector. Serum samples from 14 out of 20 patients suspected of chronic organophosphate pesticide poisoning showed peaks corresponding to ethylparathion, sumithion, and/or malathion on gas chromatography. All 15 cases of acute pesticides poisoning examined were shown to contain one, two or three kinds of organophosphate pesticides, namely ethylparathion, sumithion, malathion and/or salithion in their blood serum. On the other hand, no organophsphate pesticide was detected in the sera from control cases. Thus, detection of organophosphate pesticides in the serum contributes much to the diagnosis of poisoning from such chemicals.View full abstractDownload PDF (138K)
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