Lymphoblastoid cell lines from a patient with adenosine deaminase (ADA) deficiency and his parents were established by Epstein-Barr virus (EBV). ADA activity in the lymphoblastoid cells from the patient was not detectable, while the enzyme activity in the cells from his parents was approximately a half level of the controls'. No inhibitor to ADA could be detected in the lymphoblastoid cells from the patient.
The serum Zn content in rats bearing Yoshida-Sarcoma, AH 66F, AH 13 or AH 109A was found to be low as compared with that in normal controls. In the rats which had received intraperitoneal inoculum of Yoshida-Sarcoma, the effects of anticancer drugs were closely related to the changes in serum Zn content. Namely, serum Zn content increased and approached the normal range almost in parallel with the prolongation of survival time by anticancer drugs. As the causes responsible for the reduction in quantity of serum Zn in the tumor-bearing rats, the reduction of serum albumin content and the accumulation of Zn in the tumor tissue and organs such as the liver and kidneys were suggested.
Low molecular sialoglycoconjugates were isolated by the charcoal adsorption method from the urines of patients with mucolipidosis (one patient with mucolipidosis type I variant and three patients with mucolipidosis type II). The sialoglycoconjugates were fractionated into two major fractions (SG-1 and SG-2) by Sephadex G-25 gel filtration, and a strikingly increased excretion of the SG-1 fraction was observed in patients with these diseases. The SG-1 fraction from each type of mucolipidosis was fractionated into four sub-fractions by Sephadex G-50 gel filtration, and the fraction with the lowest molecular weight (SG-1-III) was found to contribute to the increase in the levels of SG-1. The SG-1-III fraction was further fractionated into 7 to 8 subfractions by ion-exchange chromatography. The comparison of the amounts and the chemical compositions of the subfractions suggested that the increase in SG-1 was dependent upon the increase in excretion of mannose-containing sialooligosac-charides with structures closely related to the carbohydrate units of glyco-proteins with an N-glycosidic linkage.
Serum FSH and LH in women with luteal phase defect were measured. Their serum FSH and LH were lower than those in normal women and higher than those in women with amenorrhea. Administration of clomiphene citrate to the women with luteal phase defect were effective in 2/3 of the cases. These results are consistent with the hypothesis that a relative deficiency of FSH during the follicular phase results in diminished follicular development and subsequent inadequate corpus luteum maintenance.
Morphometrical analysis of the peripheral nerve lesions in experimental diabetes rats was undertaken to elucidate their relationship to glucose intolerance and nerve conduction velocity. Motor nerve conduction velocity of the rat's tail and nerve fiber density in the cross sectional view of the peroneal nerves significantly reduced in the diabetes rats without insulin treatment. With the teased nerve fiber methods, degenerated nerve fibers frequently appeared and shortening of internodal length was estimated in the diabetes rats by quantification. The slowing of nerve conduction, blood glucose levels on the oral glucose tolerance test and the incidence of degenerated nerve fibers were well correlated with one another. Daily insulin treatment caused a significant decrease in degenerated nerve fibers in the diabetes rats. The reduction of nerve fiber density was also related to glucose intolerance. However, there was neither the relationship between nerve fiber density and incidence of degenerated nerve fibers, nor the relationship between nerve fiber density and nerve conduction velocity.
In order to elucidate receptors of proteolytic enzyme-treated red cells which react with Phaseolus coccineus L. lectin, the receptors prepared by affinity chromatography were serologically investigated. P. coccineus lectin had high agglutinin activity for bromelin-, papain- and pronase-treated red cells but that for the cells treated with ficin and trypsin was relatively low. Analyses of chemical composition revealed that sialic acid of the receptors from normal red cells was considerably much as compared with that from the treated cells. On the contrary, the enzyme treatment did not affect particularly carbohydrate composition of the receptors. Disc electrophoresis showed that the patterns of receptors from red cells treated with bromelin or papain were different from those from the other cells. On two-dimensional immunoelectrophoresis, the receptor of trypsin-treated cells gave five precipitation lines against anti-stroma and that of papain-treated cells three lines, but any other receptors showed no line. These findings indicate that there are plural receptors for P. coccineus lectin in red cells treated with each of proteolytic enzymes and that the receptors from respective red cells have electrophoretically and serologically different property.
Hemolymph from Charybdis japonica and Lymantria dispar, and saline extract from Eunice kobiensis agglutinated human group B erythrocytes specifically. Multiple agglutinins were identified, and independent agglutinins were separated from hemolymph of Charybdis japonica (anti-BCJ+anti-XCJ) and Lymantria dispar (anti-BLD+anti-RLD) by absorption experiments. By the agglutination and agglutination-inhibition experiments with simple sugars, the specificities of the anti-B agglutinins in these invertebrates were found to differ from each other.
An attempt to identify the individuality from crossed electro-immunodiffusion (CEID) patterns of serum was made by using coefficient of variation (CV), the ratio of standard deviation to mean of ratios of area in couples of corresponding peaks in the CEID patterns. The values of CV in most cases of sera from identical subject were less than 10 except only one case of 10.6. On the contrary, those in most cases of sera from different subjects were more than 20 except a few cases of 14.9 to 20. No overlap of CV values was observed between the two groups. No significant differences were demonstrated in three groups of sex pairing, i. e., male-male, female-female and female-male. From these results, analysis of CEID patterns by employing CV was found to be available for fairly definite identification as well as differentiation but useless for distinction of sexes in cases of different subjects.
Plasma renin activities (resting PRA, post-dialysis PRA and JPRA) were studied in 61 patients under chronic dialysis treatment. Removed sodium and removed water were estimated at each dialysis. ΔPRA/removed-sodium and ΔPRA/removed -water were calculated as indices in response to the removal of sodium and water during the dialysis. 1) Resting PRA (pre-dialysis PRA) was positively correlated to ΔPRA/removed-sodium, ΔPRA/removed-water, serum osmolality, and diastolic blood pressure, but negatively to serum sodium concentration, age, and pulse pressure/diastolic blood pressure. Statistically significant factors controlling the resting PRA were ΔPRA/removed-sodium, ΔPRA/removed-water, and serum sodium concentration. Resting PRA was slightly correlated to diastolic blood pressure and age. 2) Post-dialysis PRA was significantly correlated to the resting PRA, ΔPRA/removed-sodium, ΔPRA/removed-water, serum sodium concentration, and age, but not to the blood pressure indices.
When early cancer of the colon and rectum is defined as in early gastric cancer. i.e., invasion is limited to the mucosa and submucosa, 38 lesions from :35 patients of early cancer of the colon and rectum were detected during the last 20 years. The macroscopic and histologic findings included cancer-containing adenoma in 35 lesions from 32 patients. When the diagnostic methods were compared in 3:3 cases of early cancer of the colon and rectum, it was found that positive or suspicious cancer was obtained by an x-ray study in 5 of 18 cases examined (28%), by endoscopy in 19 of 31 cases (61%), by eyt.ologic methods in 18 of 21 cases (86%), by biopsy in 19 of 25 cases (76%) and by polypectomy under direct vision through an endoscope in 13 of 14 cases (93%). When both biopsy and cytologic studies were performed in combination with endoscopy (20 cases), either of them was positive in all cases. These results indicate the possibility of correct diagnosis in many cases through the combined use of the cytologic method and biopsy without polypectomy. if the location is previously checked. On the other hand, 104 polyps from 94) patients were removed by means of the snare-electrocautery technique with the use of coagulation current during the last 4 years. The histologic findings revealed cancer-containing adenoma in 13, even in benign appearing polyps and in small polyps 1.0cm or less in diameter. These results indicate that diagnosis of early cancer of the colon and rectum is difficult without polypectomy, if cancer is not previously suspected.
Severe combined immunodeficiency disease with adenosine deaminase deficiency was reported of a 1-year-2-month-old boy, who showed clinical picture of pulmonary candidiasis. The serum immunoglobulin level and absolute lymphocyte count were markedly diminished. But immunological study revealed both T-and B-cell surface markers on the peripheral lymphocytes, in spite of complete deficiency of both systems.
The neuromuscular blocking property of minocycline hydrochloride (MNC) was studied in 12 rabbits. In 2 of 6 animals, MNC, 60-160mg/kg, alone caused a decrease in twitch tension. During partial neuromuscular blockade produced by a continuous infusion of pancuronium, the intravenous administration of MNC, 20-60mg/kg, resulted in a dose-dependent decrease in twitch tension in 6 animals. MNC-induced or combined MNC-pancuronium-induced neuromuscular blockade was antagonized by calcium or by neostigmine.
A platelet kinetic study in 2 patients with acute promyelocytic leukemia (APL) associated with disseminated intravascular coagulation (DIC) demonstrated that the platelet survival was characteristically shortened. These findings suggest increased platelet consumption during the process of DIC in APL.