All issues
Volume 171 (1993)
- Issue 4 Pages 267-
- Issue 3 Pages 185-
- Issue 2 Pages 101-
- Issue 1 Pages 1-
Volume 171, Issue 4
Displaying 1-8 of 8 articles from this issue
- |<
- <
- 1
- >
- >|
-
EIJI ITOI, NEIL E. MOTZKIN, BERNARD F. MORREY, KAI-NAN AN1993 Volume 171 Issue 4 Pages 267-276
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSITOI, E., MOTZKIN, N.E., MORREY, B.F. and AN, K.-N. Bulk Effect of Rotator Cuff on Inferior Glenohumeral Stability as Function of Scapular Inclination Angle: A Cadaver Study. Tohoku J. Exp. Med., 1993, 171(4), 267-276-Eleven fresh cadaver shoulders were studied to determine the static contribution (bulk effect) of the rotator cuff on inferior glenohumeral stability provided by scapular inclination. All musculature, including the rotator cuff, was removed. The position of the humerus relative to the scapula was recorded using an electromagnetic tracking device under conditions of no force and 1.5kg of inferior translation force applied to the humerus, with the arm in the hanging position (sulcus test) and then in 90° abduction (Abduction-Inferior Stability test=ABIS test), with the scapula inclined referable to the vertical line at -15°, 0°, 15° and 30° in the sulcus test and at 15°, 30°, 45° and 60° in the ABIS test. In the sulcus test without load, all shoulders dislocated at scapular inclination angles of -15° and 0°, whereas no shoulders dislocated at 30°. The angle of scapular inclination had a significant effect on humeral head positions (p<0.0001), with the head position at -15° and 0° being lower than at 15°, which was lower than at 30°. In the ABIS test, none of the shoulders dislocated, although the effect of the angle of scapular inclination was significant (p<0.0001), with the position of the humeral head being higher at 15° than at other angles of inclination. Comparison of these data and previously reported data with the cuff intact showed no significant effect of rotator cuff removal on humeral head position and displacement in both tests. Therefore, we conclude that the static condition of the rotator cuff has no significant effect on the stabilizing function of scapular inclination. The stabilizing mechanism of scapular inclination seems to be associated with the bony configuration and/or anatomy and biomechanical properties of the superior capsuloligamentous structures.View full abstractDownload PDF (930K) -
AKIRA ISHIDA, YUKIO SAWAISHI, ATSUKO GOTO, YASUSHI TAKAHASHI, HIROKAZU ...1993 Volume 171 Issue 4 Pages 277-283
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSISHIDA, A., SAWAISHI, Y., GOTO, A., TAKAHASHI, Y., ARAI, H., NAKAJIMA, W., ONOZAKI, M. and TAKADA, G. Two Siblings with Partial Trisomy 15 and Monosomy 21 Associated with Central Nervous System Anomalies. Tohoku J. Exp. Med., 1993, 171 (4), 277-283-A sister and a brother with 46, XX (46, XY), -21, +der (15) (q22.1; g22.1) mat were reported whose mother had a karyotype of 46, XX, t(15; 21)(q22.1; 22.1) and was phenotypically normal. Both sibs were mentally retarded and dysmorphic. Moreover, the sister had a holoprosencephaly with congenital hydrocephalus, and the brother showed congenital hydrocephalus.View full abstractDownload PDF (1257K) -
HIDETOSHI KOTAKE, SHINICHI OIKAWA, TAKASHI NAITO, KYOKO HAYASAKA, TAKA ...1993 Volume 171 Issue 4 Pages 285-295
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKOTAKE, H., OIKAWA, S., NAITO, T., HAYASAKA, K. and TOYOTA, T. The Effect of Serum on Low Density Lipoprotein Metabolism of Human Monocyte- Derived Macrophages. Tohoku J. Exp. Med., 1993, 171(4), 285-295-Macrophages oxidatively modify low density lipoprotein (LDL). These cells then recognize and take up oxidized LDL (OxLDL) via the scavenger receptor. In the present study, we examined the effect of lipoprotein depleted serum (LPDS) on the oxidative modification of LDL and its uptake and degradation by human monocyte-derived macrophages. LPDS inhibited LDL oxidation in a concentration-dependent manner and the complete inhibition was observed in the concentration from 2.5 to 10.0%. In the presence of 5% of LPDS, cell-associated 125I-LDL was reduced by 70%, compared with that in the absence of LPDS. In contrast, 125I-LDL degradation increased in the presence of LPDS. The ratio of degradation products to cell-associated radioactivity in the presence of LPDS was five-fold greater than that in the absence of LPDS. In the presence of LPDS, unlabeled LDL competed for about 50% of cellullar uptake and degradation of 125I-LDL. In the absence of LPDS, however, unlabeled LDL competed for only 20% of uptake and only 30% of degradation of 125I-LDL. Unlabeled OxLDL competed for about 80% of uptake and degradation in the absence of LPDS. These results suggest that LPDS inhibited the production of OxLDL which was taken up by macrophages via the pathway for the OxLDL.View full abstractDownload PDF (1107K) -
NAOKI TAMASAWA, MASASHI YONEDA, ISAO MAKINO, KAZUO TAKEBE, KEN SONE, R ...1993 Volume 171 Issue 4 Pages 297-307
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSTAMASAWA, N., YONEDA, M., MAKINO, I., TAKEBE, K., SONE, K, and KOGAWA, R. The Effect of Biliary Bile Acid Concentration and Composition on the Calcium Level in Human Gallbladder Bile. Tohoku J. Exp. Med., 1993, 171(4), 297-307 -We analyzed total and ionized calcium concentrations in gallbladder bile of 34 humans in four groups: 8 patients with no gallstone, 11 gallstone patients treated with no gallstone dissolution agents, 8 gallstone patients treated with chenodeoxycholic acid(CDCA) and 7 gallstone patients treated with ursod eoxycholic acid(UDCA). We found that total calcium level ranged from 1.40 to 8.01 mmol/liter, closely related to total bile acid concentration (r=0.759). However, ionized calcium level was maintained in a narrow range of 0.25 to 1.23mmol/liter and had no relation to total bile acid concentration. UDCA-rich bile showed relatively high level of ionized calcium. We performed ultrafiltration of bile with cut-off molecular weight 1, 000 to investigate the interaction between biliary calcium and bile acid aggregates. The proportion of ultrafiltrated bile acid level to that in original bile in the UDCA group was statistically higher than the other groups. Relatively large percentage of smaller bile acid aggregates in UDCA-rich bile may impair its calcium solubility.View full abstractDownload PDF (1137K) -
SHUICHI SATO, MIKI YAMADA, YUTAKA MIYAZAKI, KEIZABUROH MATSUDA, ATSUSH ...1993 Volume 171 Issue 4 Pages 309-317
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSATO, S., YAMADA, M., MIYAZAKI, Y., MATSUDA, K., KANNO, A., ISHII, M., OHORI, H. and TOYOTA, T. Relationship between Molecular State of Serum HBV-DNA and Clinical Features of Hepatitis B Virus Carriers. Tohoku J. Exp. Med., 1993, 171(4), 309-317-Molecular species of serum hepatitis B virus (HBV)-DNA in HBV carriers were classified by Southern blot hybridization into three types; type I with two bands of 4.0kb and 3.2kb, type II with the two bands of type I plus the smear between 4.0kb and 3.2kb, and type III with a broad band below 4.0kb. A total of 51 HBV carriers were classified into three groups (group I, n=19 with type I; group II, n=12 with type II; and group III, n=20 with type III). Serum aminotransferase levels of group I were significantly lower than those of groups II and III. Liver pathology revealed that 18 of the 19 (94.7%) group I patients showed nonspecific reactive hepatitis (NSRH), while 11 of the 12 (91.7%) group II patients and 19 of the 20 (95.0%) group III patients showed chronic persistent hepatitis (CPH) or chronic active hepatitis (CAH). Immunohistochemical study showed that hepatitis B core antigen (HBcAg) was localized in the nucleus of hepatocytes in most of patients with type I while it was localized in both the nucleus and cytoplasm in those with types II and III. Since the smear between 4.0kb and 3.2kb is specifically found in groups II and III, HBV-DNA with this smear may be related to active hepatitis.View full abstractDownload PDF (1503K) -
TERUO NAKAMURA, KAZUO TAKEBE, KENJI KUDOH, NAOKO YAMADA, AKINORI TERAD ...1993 Volume 171 Issue 4 Pages 319-325
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSNAKAMURA, T., TAKEBE, K., KUDOH, K., YAMADA, N., TERADA, A., ISHII, M., ARAI, Y., IMAMURA, K., MACHIDA, K. and KIKUCHI, H. A Study on the Values Computed by Dieticians and Chemical Analysis of Fats, Cholesterol, and P/S Ratio in Food. Tohoku J. Exp. Med., 1993, 171(4), 319-325-Dieticians computed the fat and cholesterol contents of 11 foods that were commercially produced as ready-to-eat food from food component lists and obtained the P/S ratio (polysaturated/saturated fatty acids) from the fatty acid component list. Meanwhile the same foods were diluted and homogenized. The internal standard was combined with heptadecanoic acid and tricaprin. The samples that had been extracted by the Folch method were analyzed for their lipid content (GC analysis using a HS-SS-10 colums for fatty acids and an OV-1 column for lipid and cholesterol). A significant positive correlation was noted between the results of dieticians' analysis and those obtained from a gas chromatographic analysis of lipid and cholesterol contents and the P/S ratio, proving that lipid analysis of food by dieticians is highly reliable. Therefore for diseases (such as hyperlipemia, arteriosclerosis, obesity, diabetes mellitus, fatty liver, and pancreatitis) in which dietary factors have a significant effect on their clinical course, dietary instructions on dietary fats based on an analysis by dieticians are considered to be effective.View full abstractDownload PDF (486K) -
TOSHIO KUDO, SUSUMU SAIJYO, HISAAKI SAEKI, NOBUYUKI SATO, TAKEHIKO TAC ...1993 Volume 171 Issue 4 Pages 327-338
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKUDO, T., SAIJYO, S., SAEKI, H., SATO, N., TACHIBANA, T. and HABU, S. Production of a Human Monoclonal Antibody to a Synthetic Peptide by Active In Vivo Immunization Using a SCID Mouse Grafted with Human Lymphocytes. Tohoku J. Exp. Med., 1993, 171(4), 327-338-In order to meet the present increased demands, we have tried to improve the methods to produce human monoclonal antibodies by using a SCID mouse grafted with human mononuclear cells. Initially, we gave an anti-asialo GM1 antibody to a SCID mouse to suppress the NK activity, as a pretreatment. Then, fifty million human mononuclear cells (MNC) from a healthy volunteer were injected intraperitoneally to the SCID mouse so as to construct a human immune system in the mouse (PBL-SCID mouse). We immunized the mouse with a synthetic peptide (pep 190) conjugated with KLH four times. The spleen cells taken from the immunized PBL-SCID mouse were fused with (mouse×human) heteromyeloma cells. The hybridoma cells were selected in GIT medium containing HAT and IL-6. Among 68 hybridoma-growing wells, we obtained one hybridoma clone (#41-1-4) which secreted a specific antibody to pep 190. The reactivity of this monoclonal antibody was tested by ELISA and the specificity of this antibody was confirmed by an absorption test with different kinds of proteins. This paper is the first report of the successful production of peptide-specific human monoclonal antibody by active in vivo immunization using a PBL-SCID mouse. By this active in vivo immunization system using a PBL-SCID mouse, human monoclonal antibodies for any sort of peptide antigens may easily be made available.View full abstractDownload PDF (1272K) -
YOSHITAKA TSUBONO, AKIRA FUKAO, SHIGERU HISAMICHI1993 Volume 171 Issue 4 Pages 339-348
Published: 1993
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSTSUBONO, Y., FUKAO, A. and HISAMICHI, S. Health Practices and Mortality in a Rural Japanese Population. Tohoku J. Exp. Med., 1993, 171(4), 339-348-Association of personal health practices with mortality from all causes was studied. In 1988, 4, 318 residents aged 40 years and over in a rural town of Miyagi Prefecture, north-eastern part of Japan, completed a self-administered questionnaire including items on smoking, alcohol consumption, physical activity, sleeping patterns, and weight status. During the four year follow-up, 207 subjects had died. Relative risks (95 percent confidence intervals) of death from all causes adjusted for age, sex, and other confounding variables were 0.62 (0.39-0.98) for never smoking, 0.51(0.34-0.78) for no or moderate alcohol consumption, 0.66 (0.42-1.04) for exercising physical activity, 0.78 (0.56-1.10) for 7-8 hours of sleep, and 0.75 (0.52-1.07) for not being underweight. Compared with performing only 0-1 of the five low-risk practices concerning the items mentioned above, relative risks (95 percent confidence intervals) of all-cause mortality for performing 3, or all 5 practices were, 0.49 (0.30-0.80) and 0.14 (0.03-0.61), respectively (trend p= 0.0001). The results remained unchanged even after excluding early death occurring within the first year of the follow-up, or excluding the subjects with past history of diseases. Further research is required to explore the combined effects of the practices on mortality for longer observational period.View full abstractDownload PDF (1037K)
- |<
- <
- 1
- >
- >|