Nageotte gained success in making silver fibers artificially on a slide glass by addying a proper amount of NaCI to an albumin
sol, Collagen A which he had obtained after treating tendons of a rat's tail with a dilute solution of glacial acetic acid. No cells were participant of this creation. After-experiments could confirm the result of his experiment, but the suc-cess rate was found extremely low. In my early tests when I adopted- the same method of his, it was only in one specimen out of about one hundred that I could see somewhat good result. In addition, these fibers were slenderer in shape, smaller in number and poorer in dyeing acceptability as compared with those
in vivo.
Thereupon, I examined into the essential factors of the artificial forma-xion of fibers and in consequence got a strong confirmation to the under-mentioned facts.
The formation of these fibers is conditioned by the same general factors which allow the
sol to change into
gel when the albumin coagulation is brought about. These factors are the thickness and the pH of Collagen A, the kind and the thickness of salt that the
sol is treated with, and the temperature at the time of coagulation. When experiments are carried on with these five factors in their satisfactory conditions, fibers are sure to appear on the slide-glass. And what is more, the fibers formed are abund-ant and have the perfect uniformity in their dyeing acceptability and from with those silver fibers found in an organic body (shown in the photos).
Following is an abridged description of my practical experiments. Before carrying out an experiment on a slide glass, I inquired in the neces-sary conditions of the coagulation of Collagen A in test tubes.
I pour 0.1 cc. of a salt solution of different degrees of thickness into some ten tubes respectively where 0.9cc. of Collagen A has been placed. Having been given a good deal of shaking, the fluid is kept at a certain constant temperature (20-30°C). After 10-30minutes, I examine all the tubes to find some in which a whitish turbildity is clearly visible with my naked eye. I repeat this experiment on slide glasses instead of in tubes with the same process and under the same conditions. Only this time the salt solution needs to be as thick or thicker than that used in the foregoing test in tubes. Fibers are to come out without fail. This method can, with the most successful result, be applied practically to any other salt than NaCl. Moreover, the fibers formed are splendid enough to be com-pared with those
in vivo and we can produce them whenever we will.
These fibers produced in our laboratories take on a great variety of form and acceptability of dyes. But they may be classified into three major classes. The fibers of type A assume a membraneous form and are dyed well with aniline blue or acid fuchsin, but colored light black or reddish purple with silver. Those of type B form a lattice-like network and those of type C are like threads, which gather together, taking the form of a textile fabric of felt. Both the last two types are rich in argyrophilis and colored jet-black with silver. They are dyed comparatively well with aniline blue, but not so well with acid fuchsin.
As has been mentioned above, there are various forms of fibers pro- duced, but as their forming conditions are clearly known in respective cases, we can experimentally make generate whichever sort of fibers of our own free will. When we use a salt solution of coagulation value, that is, of the lowest thickness enough to make Collagen A begin to coagulate, fibers belonging to type A are the most cases, and according as the solution increases in thickness, there appear those of type B and then those of type c.
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