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Volume 174 (1994)
- Issue 4 Pages 305-
- Issue 3 Pages 181-
- Issue 2 Pages 95-
- Issue 1 Pages 1-
Volume 174, Issue 2
Displaying 1-8 of 8 articles from this issue
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KIYOSHI ISHIYAMA, SHINJI SATOH, YOSHIHARU IGARASHI, HIROAKI KUMAGAI, A ...1994 Volume 174 Issue 2 Pages 95-107
Published: 1994
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSISHIYAMA, K., SATOH, S., IGARASHI, Y., KUMAGAI, H., YAHAGI, A. and SASAKI, H. Flow Cytometric Analysis of the Cell Cycle of the Leukemic Cell Lines Treated with Etoposide and Cytosine Arabinoside. Tohoku J. Exp. Med., 1994, 174 (2), 95-107-Effects of etoposide (VP-16) and cytosine arabinoside (Ara-C) on the cell cycle of HL-60 and THP-1 cells were studied by flow cytometry using the bromodeoxyuridine (BrdU)/DNA assay technique to investigate the efficacy of VP-16 for monocytic leukemia cells. VP-16 inhibited the proliferation of THP-1 cells more strongly than that of HL-60 cells at any concentrations used at 24 and 48hr. VP-16 arrested HL-60 and THP-1 cells in the G2/M phase and reduced them in the G0/G1 and early S phase at higher concentrations. There was no significant difference in the percentage of G2/M phase cells at the same concentration between both cells. However, reduction in the G0/G1 and early S phase cells was more marked in THP-1 than HL-60 cells significantly. On the other hand, Ara-C perturbed the cell cycle of HL-60 cells more than that of THP-1 cells at 24 and 48hr. These results suggest that the effects of VP-16 on the cell cycle may be more intense in THP-1 than HL-60 cells, and support the efficacy of VP-16 for treating monocytic leukemia in vivo.View full abstractDownload PDF (1102K) -
KIYOTAKA NUKII, YOSHIKI KAKIZAKI, SHINOBU WAGA1994 Volume 174 Issue 2 Pages 109-118
Published: 1994
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSNUKII, K., KAKIZAKI, Y. and WAGA, S. Effect of Serum from Patients with Mesangial Proliferative Glomerulonephritis on Cultured Rat Mesangial Cells. Tohoku J. Exp. Med., 1994, 174 (2), 109-118-We studied the effect of serum from patients with renal diseases characterized by mesangial proliferation on the proliferation of rat mesangial cells (MC) in culture. Indirect immunofluorescence technique with human anti-proliferating cell nuclear antigen (PCNA) antibody was utilized to detect proliferating MC in S-phase on microscope slides. MC from serum-starved culture medium containing 0.5% fetal calf serum (FCS) showed 6± 2% of PCNA-positive MC, whereas those from complete medium containing 20% FCS showed 45±7%. This was confirmed by flow cytometry for MC from both conditions, in which 10.9% and 55.2% of S-phase MC were detected, respectively. Serum from 17 patients with IgA nephropathy as a prototype of mesangial proliferative glomerulonephritis produced more PCNA-positive MC than serum from 18 healthy controls ( mean±S.D.=21.5±9.9% vs. 4.4±5.9%, p<0.001). In IgA nephropathy, this effect correlated tentatively with active histologic lesion in the glomeruli, but not with severity of urinary abnormalities. Serum from membranoproliferative glomerulonephritis (MPGN) also produced significant PCNA-positive MC ( mean±S.D.=15.6±9.3%), compared to controls (p<0.01), independent of the severity of urinary abnormalities. These findings suggest that serum effect on the proliferation of cultured rat MC may reflect an active histologic lesion in the glomeruli, and that anti-PCNA antibody to detect proliferating MC is, if not quantitative, relevant methodology to enumerate proliferating MC in culture.View full abstractDownload PDF (1289K) -
CHIKASHI SHIBATA, IWAO SASAKI, HIROO NAITO, NORIYA OHTANI, SEIKI MATSU ...1994 Volume 174 Issue 2 Pages 119-128
Published: 1994
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSHIBATA, C., SASAKI, I., NAITO, H., OHTANI, N., MATSUNO, S., MIZUMOTO, A., IWANAGA, Y. and ITOH, Z. Effects of Substance P on Gastric Motility Differ Depending on the Sites and Vagal Inneruation in Conscious Dogs. Tohoku J. Exp. Med., 1994, 174 (2), 119-128-The effect of substance P on gastric motility was studied in conscious dogs by means of strain gauge force transducers chronically implanted on the gastric body, antrum, and a vagally-denervated fundic pouch. Intravenous infusion of substance P in the interdigestive state induced phasic contractions in the pouch and antrum. Atropine inhibited these contractions in the pouch and antrum. Hexamethonium enhanced substance P-induced contractions in the gastric antrum, but reduced those in the pouch. Pretreatment with phentolamine, propranolol, or naloxone did not affect substance P-induced contractions in the pouch and antrum. The intact gastric body scarcely reacted to substance P. Mean systemic blood pressure was lowered by substance P-infusion, but there was no dose-dependency in the reduction of the blood pressure, nor was it affected by the pretreatment with atropine or hexamethonium. These results suggest that 1) the vagal innervation influences the effect of substance P on motility in the gastric body, and that 2) substance P may stimulate postsynaptic excitatory cholinergic and presynaptic inhibitory neurons simultaneously in the gastric antrum.View full abstractDownload PDF (947K) -
TAKASHI SAWAI, JUN-ICHI FUJIYAMA, MITSUHIRO TAKAHASHI, TOHRU TAKAHASHI1994 Volume 174 Issue 2 Pages 129-140
Published: 1994
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSAWAI, T., FUJIYAMA, J., TAKAHASHI, M. and TAKAHASHI, T. The Site of Elevated Vascular Resistance in Early Paraquat Lungs: A Morphometric Study of Pulmonary Arteries. Tohoku J. Exp. Med., 1994, 174 (2), 129-140 - In interstitial lung diseases the pulmonary vascular resistance is more or less elevated. Although this usually is attributed to collapse of capillary bed by fibrosis, vasoconstriction of pulmonary arteries may be another important mechanism. Segments of pulmonary arteries supplying fibrotic areas, if subjected to hyperreactivity and overstraining of the wall, are expected to precipitate thickening of muscular media, revealing when and where vasoconstriction takes place. This was examined by morphometry of arteries in autopsy lungs from 21 patients dying in various stages of fibrosis, with five normal lungs as control. In microscopic lung slides, crosssectioned pulmonary arteries were submitted to the measurement of DM, the medial thickness, and R, the radius, standardizing variously shrunken vessels at a circularly stretched elastic membrane. In each case, ten to thirty arteries were measured so as to cover a wide range of R from 50 to 1, 000μm. In all cases, there was a linear log-log correlation between R and DM. In paraquat lungs, DM began to rise as early as the 8th day, i.e., almost simultaneously with beginning deposition of fibrogenic matrix on alveolar wall, suggesting that the medial hypertrophy is the result of hypoxic vasoconstriction due to alveolar-capillary block. Medial thickening was the strongest in small arteries of acinar level. Hypoxic vasoconstriction of pulmonary arteries is likely to occur in an early stage of fibrotic lung disease and contribute to elevated vascular resistance. The intra-acinar small arteries are most liable to respond.View full abstractDownload PDF (1535K) -
TAKASHI SAWAI, KAZUHIRO MURAKAMI, YUKIKO KURASONO1994 Volume 174 Issue 2 Pages 141-154
Published: 1994
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSAWAI, T., MURAKAMI, K. and KURASONO, Y. Morphometric Analysis of the Kidney Lesions in Mixed Connective Tissue Disease (MCTD). Tohoku J. Exp. Med., 1994, 174 (2), 141-154-We examined histopathological changes of the kidney in patients with mixed connective tissue disease (MCTD) including those of glomeruli, arteries and interstitium by morphometric method. All specimens examined were collected from 25 autopsy cases diagnosed as MCTD according to the criteria for this disease proposed by the MCTD committee sponsored by the Japanese government. Clinical evidence of renal dysfunction such as proteinuria was present in 16 out of 25 cases (64%). Histopathologically, membranous type glomerular lesion was found most frequently (40%), followed by membranoproliferative (6.7%) and mesangioproliferative types (6.7%). Nine cases had no glomerular lesion. Severe arterial lesion such as necrotizing angiitis was not found in our kidney specimens. However, morphometry revealed a high incidence of intimal thickening in the renal arteries of these patients as compared to control cases, showing this to be one of the most common features of MCTD with clinical importance. This type of arterial lesion, also seen in kidneys in other types of collagen diseases, may suggest an etiology common to them. The severity of the renal interstitial lesion in MCTD was intermediate between that of systemic lupus erythematosus (SLE) and progressive systemic sclerosis (PSS), poly-or dermatomyositis (PM/DM).View full abstractDownload PDF (1634K) -
MINORU NAKAZATO1994 Volume 174 Issue 2 Pages 155-165
Published: 1994
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSNAKAZATO, M. Human Prostate Acid Phosphatase is Carrier Protein for ABH Blood Group Antigens of Semen. Tohoku J. Exp. Med., 1994, 174 (2), 155-165 - Prostate acid phosphatase (PAcP) was characterized as one of carrier proteins for ABH blood group antigens in semen. The diluted semen was sensitized with anti-PAcP. The resulting immunocomplex was subjected to SDS-PAGE after being washed with saline. SDS-PAGE patterns of the immunocomplex consisted of four bands. After electroblotting from gel to nitrocellulose filter, immunostaining using anti-PAcP or monoclonal anti-blood group antibodies was performed. The result showed that the protein of 50kD was the subunit of PAcP and the band of 94kD was identified with blood group antigens respectively. Other two bands were rabbit IgG against PAcP and its fragment. When semen was treated with the chemicals such as papain, SDS, 2-mercaptoethanol and dithiothreitol, the ABH of antigen which was captured by anti-PAcP were considerably reduced. The reduction of ABH activity by these treatments indicated that ABH antigens were located on PAcP in semen which was inactivated by the treatment. Therefore, PAcP was one of proteins carrying ABH epitopes in semen in addition to α2-seminoglycoprotein.View full abstractDownload PDF (2437K) -
SETSUKO KAWAMURA, MASAMI YOSHIIKE, TADASHI SHIMOYAMA, YUHKO SUZUKI, JU ...1994 Volume 174 Issue 2 Pages 167-175
Published: 1994
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKAWAMURA, S., YOSHIIKE, M., SHIMOYAMA, T., SUZUKI, Y., ITHO, J., YAMAGATA, K., FUKUSHIMA, K., OGASAWARA, H., SAITOH S., TSUSHIMA, K., SAWADA, Y., SAKATA, Y. and YOSHIDA, Y. Management of Acute Leukemia during Pregnancy: From the Results of a Nationwide Questionnaire Survey and Literature Survey. Tohoku J. Exp. Med., 1994, 174(2), 167-175 - In March, 1993, a questionnaire was sent to 362 gynecological and obstetric offices of national, prefectural and municipal hospitals and private university hospitals with 250 beds or more. Answers were collected from 260 institutions. Thus, this study analyzed 39 patients with acute leukemia during pregnancy collected by the questionnaires survey and 64 cases reported in the Japanese literatures during 1975-1993 (total 103 patients). The weeks of pregnancy were defined as the 1st (<15th week), 2nd (16th-27th week), and 3rd (>28th week) trimesters. The time of dagnosis of leukemia during pregnancy changed from 25% in the 2nd trimester and 62% in the 3rd trimester during 1975-1984 to 39% and 48% after 1985, respectively. After 1985, the remission rate was 72% in the questionnaire group and 75% in the group from literatures. There was no statistical difference. The 50% survival period was 12 months in the group during 1975-1984, but 25 months in the group after 1985. The survival was significantly longer in the patients whose induction therapy was started before delivery than in those treated after delivery The results suggest that the treatment for acute leukemia during pregnancy should be initiated as soon as possible after the diagnosis of leukemia, with carefully selected regimens. It is important that the time of delivery should be selected considering the maternal and fetal conditions after consultation with an obstetrician.View full abstractDownload PDF (785K) -
WEN SU, XIAO YOU HAN, YA PING WANG, YU LUAN WANG, YAO WEN ZHN, TAKAFUM ...1994 Volume 174 Issue 2 Pages 177-180
Published: 1994
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSU, W., HAN, X.Y., WANG, Y.P., WANG, Y.L., ZHN, Y.W., SASABA, T., HOSHIYAMA, Y. and TAGASHIRA, Y. Joint Risks in a Case-Control Study of Esophageal Cancer in Shanxi Province, People's Republic of China. Tohoku J. Exp. Med., 1994, 174 (2), 177-181 - A multifactorial analysis on the etiology of esophageal cancer was conducted based on a case-control study conducted in Shanxi. The study analyzed the data of 326 cases and 396 controls. The joint risks of two factors were calculated from dichotomous distributions. Three models of factor combinations were assessed: (1) two risk-enhancing factors, (2) two risk-reducing factors, and (3) a risk-enhancing and a risk-reducing factor. The observed joint risks were in the neighborhood of the multiplicative products of single acting risks of individual factors. This was a uniform pattern across three models.View full abstractDownload PDF (364K)
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