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Volume 108 (1972)
- Issue 4 Pages 307-
- Issue 3 Pages 199-
- Issue 2 Pages 101-
- Issue 1 Pages 1-
Volume 108, Issue 4
Displaying 1-10 of 10 articles from this issue
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YUNOSUKE OGAWA1972 Volume 108 Issue 4 Pages 307-315
Published: 1972
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe phospholipids in the amniotic fluid from healthy pregnant ewes at various stages of gestation were analyzed in relation to the fetal pulmonary surfactant. The most abundant phospholipid was phosphatidylcholine which increased as the term approached. Analysis on the positional specificity of amniotic phosphatidylcholine revealed a similar fatty acid composition to the surfactant phosphatidylcholine of the fetal lung. The increase in disaturated phosphatidylcholine concentration of amniotic fluid during gestation correlated well with the increase in the surfactant phospholipid of fetal lungs. These results indicate the presence of pulmonary surfactant of the fetal origin in the amniotic fluid, and serve as a basic value for the antenatal diagnosis of idiopathic respiratory distress syndrome.View full abstractDownload PDF (572K) -
YOSHISUKE MARUHAMA, AKIRA YANBE, AKIRA OHNEDA, SHOICHI YAMAGATA1972 Volume 108 Issue 4 Pages 317-325
Published: 1972
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSA simple method for separation of plasma chylomicron and eolorimetric determination of its triglyceride content was described. Chylomicron in plasma, diluted appropriately, was collected by ultrafiltration using cellulose nitrate membrane filter with pore size of 100 mμ. Chylomicron in residue was extracted with chloroform and triglyceride-glycerol was measured. The mean recovery rate of chylomicron-triglyceride was 88.6%. The relative error was 7.6% and there was no loss of accuracy for plasma from patients with gross hyperchylomicronemia. An analysis using paper electrophoresis revealed validity of cylomicron separation by the filtration method. An adequate dilution of plasma could avoid packing on the membrane filter and therefore over-estimation. The mean value of fasting chylomicron-triglyceride was 4.8±1.1mg/100ml (±SE) in 15 normal subjects, 12.2±2.0mg/100ml in 18 moderate diabetics and 30.9±5.0mg/100ml in 13 severe diabetics.View full abstractDownload PDF (941K) -
YOICHIRO SASAI1972 Volume 108 Issue 4 Pages 327-336
Published: 1972
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe graphical method of Lineweaver-Burk to the histochemical system was applied to obtain quantitative information on the lactic dehydrogenase activity in human epidermis. In vitro experiment showed that the incubation time necessary for yielding a certain amount of formazan is related to the amount of enzyme present. In the case of section experiment, the time required for the first appearance of formazan deposition in the tissue at various substrate concentrations was plotted against the reciprocal of the substrate concentration. This technique made it possible to compare the lactic dehydrogenase activities at various sites of the tissue.View full abstractDownload PDF (550K) -
YUKIO KIMURA, KOYO MIYATA, KUNIAKI ADACHI, SATOSHI MATSUMURA1972 Volume 108 Issue 4 Pages 337-352
Published: 1972
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe effects of autonomic drugs on seminal emission and ejaculation were investigated in mongrel dogs. Changes in the posterior urethral pressure (posterior urethrogram) and the volume of seminal emission during continuous hypogastric nerve stimulation were measured in test animals. The seminal emission induced by hypogastric nerve stimulation caused continuous rise in the posterior urethral pressure. When the pressure reached the maximum level, rhythmic alterations of the pressure which was considered, to be a phenomenon associated with ejaculation occurred. The drugs were administered into the abdominal aorta just above its bifurcation. Tetrodotoxin (1-5 μg) abolished both seminal emission and rhythmic alterations of the posterior urethral pressure. Phenylephrine (10-100 μg) and methoxamine (10-30 μg) increased markedly the seminal emission and induced the rhythmic alterations. Phentolamine (1-10mg) and phenoxybenzamine (1-10mg) abolished both seminal emission and the rhythmic alterations. Administration of isoproterenol (10-30 μg) or propranolol (1-10mg) affected neither seminal emission nor rhythmic alterations. Acetyl-choline (1-10 μg) or atropine (1-10 μg) did not show any definite effect on both seminal emission and the rhythmic alterations. No significant effect of DMPP (10-30 μg) on seminal emission and rhythmic alterations were observed. No significant change in seminal emission was caused by hexamethonium (100-500 μg). The rhythmic alterations were not affected by this agent in many dogs. From these results it is concluded that both seminal emission and ejaculation are predominantly under the influence of adrenergic nervous system, particularly through an a-adrenergic receptor mechanismView full abstractDownload PDF (7007K) -
YASUHIKO FURUTA, KAZUHIKO IWATSUKI, OSAMU TAKEUCHI, KOROKU HASHIMOTO1972 Volume 108 Issue 4 Pages 353-360
Published: 1972
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe composition of the pancreatic secretion was analyzed when the secretion was increased by intravenous infusions of dopamine from 0.03 to 0.5mg/min, highly purified secretin (Jorpes) from 0.03 to 0.5 U/min and pancreozymin (Boots) from 0.03 to 0.5 U/min in dogs. Dopamine and secretin caused the production of a large volume of juice containing a high concentration of bicarbonate but had little effect on protein output. On the other hand, pancreozymin caused a marked increase in output of total protein but little increase in the secretion and bicarbonate concentration. The effects of dopamine from 0.03 to 0.5mg/min i. v. on the secretory volume and the bicarbonate excretion roughly corresponded to those of secretin from 0.03 to 0.5 U/min i. v. The activities of lipase, amylase and trypsin per unit volume (=1 ml) of the juice obtained with pancreozymin (0.3 to 0.5 U/min) were higher than those with either dopamine (0.3 to 0.5mg/min) or secretin (0.3 to 0.5 U/min). These experiments show that dopamine has a secretin-like action on the pancreatic secretion.View full abstractDownload PDF (413K) -
SHIGERU ARAI, ATSUSHI MACHIDA1972 Volume 108 Issue 4 Pages 361-367
Published: 1972
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe total number of myocardial cells of the left ventricle of normal and hypertrophied hearts was histometrically evaluated in autopsy cases. In the postnatal life it was kept constant both in physiological and pathological growth of the heart. It was concluded that cardiac hypertrophy was induced by geometrically similar enlargement of individual myocardial cells. The role of amitoses of the myocardial nuclei in numerical increase of the heart muscle cells was rather doubtful.View full abstractDownload PDF (398K) -
TADAO ORII, RYOJI MINAMI, TOORU NAKAO1972 Volume 108 Issue 4 Pages 369-375
Published: 1972
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSQualitative and quantitative determination of urinary acid mucopolysaccharide has been studied by several methods including ECTEOLA-cellulose column chromatography, Dowex 1×2 column chromatography, electrophoresis, and enzymatic digestion with testicular hyaluronidase and chondroitinase. To avoid the possibility of loss of urinary acid mucopolysaccharides, especially of keratosulfate, cetylpyridinium chloride as an acid mucopolysaccharide precipitant and dialytic procedure were not used. 1) The normal male children excreted 8.0mg of uronic acid and 8.5mg of hexosamine/ liter of urine, and the uronic acid (mg)/creatinine (g) ratio was 12.8 and the hexosamine (mg)/creatinine (g) ratio was 13.8. 2) The urinary acid mucopolysaccharides in the normal male children comprised mostly chondroitin sulfate A (47.6%) and chondroitin sulfate C (34.2%). 3) Non-sulfated acid, mucopolysaccharides comprised 10.4% of the total acid mucopolysaccharide excreted, consisted of hyaluronic acid (3.3%) and chondroitin (7.1%) respectively. 4) The remaining acid mucopolysaccharides consisted of heparitin sulfate (3.4%), chondroitin sulfate B (2.8%) and keratosulfate (1.6%).View full abstractDownload PDF (414K) -
SUSUMU SATOH, OSAMU TAKEUCHI, KOROKU HASHIMOTO1972 Volume 108 Issue 4 Pages 377-388
Published: 1972
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSResponses of the dog submaxillary gland and its vascular bed to various compounds administered into the glandular branch of the facial artery were investigated under the constant perfusion pressure. Cholinergic drugs such as acetylcholine, bethaneehol, methacholine, carbachol, pilocarpine, physostigmine and neostigmine; ganglionic stimulants such as nicotine, DMPP, lobeline, TMA and McN-A-343; β-adrenergic stimulant such as isoproterenol; and biogenic amines such as histamine and 5-hydroxytryptamine, produced a salivary secretion and dilation of the glandular vascular bed. Adrenergic drugs catehcholamine releaser such as epinephrine, norepinephrine, dopamine, phenylephrine, tyramine and methoxamine, caused a salivary secretion and vasoconstriction. Although either vasoconstrictors such as angiotensin II, lys-vaso-pressin, oxytocin, TEA, cocaine and ergotamine, or vasodilators such as bradykinin, kallikrein, secretin, panereozymin, gastrin, adenosine, AMP, ADP, ATP, uridine, UMP, UDP, UTP, DPN, TPN, nitroglycerin, papaverine, dipyridamole, verapamil, hydralazine, caffeine, theophilline, theobromine, morphine, procaine and tolazoline, produced prominent vascular effects, they exerted no effect on the salivary secretion. There is no evidence on the direct relation between secretagogue and vasodilating effects of drugs which are administered selectively into the submaxillary glandular artery.View full abstractDownload PDF (635K) -
SHOICHI IMAI, YUMI KATANO, KEISUKE TAKEDA, MAMORU KUMADA, HIROMI IIZUK ...1972 Volume 108 Issue 4 Pages 389-399
Published: 1972
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe cardiac actions of peruvoside, a monoglycoside obtained from the seeds of Thevetia neriifolia, were analyzed, based on the principle of the two-compartment model system, in the dog heart-lung preparations in a precise quantitative manner and were compared with those of g-strophanthin and proscillaridin. The therapeutic dose of peruvoside was found to be about 13% of the irregularity dose and about 8% of the lethal dose, as compared with 25% and 13% for g-strophanthin, and 60% and 40% for proscillaridin, respectively, indicating that peruvoside had the widest margin of safety among the three compounds tested. Validity of using the two-compartment model system as the basis for the quantitative comparison of cardiac actions of the cardiotonic steroids was discussed.View full abstractDownload PDF (584K) -
KINYA MOCHIZUKI1972 Volume 108 Issue 4 Pages 401-409
Published: 1972
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSStroma-free hemolysate in 53 cases of morbus basedowii was examined by starch-gel electrophoresis. Hemoglobin A1, A2 and carbonic anhydrase B (CA-B) were separated, and stained with bromphenol blue. CA-B quantity was densitometrically measured, and expressed as per cent to total hemoglobin. The results were as follows: CA-B quantities of normal adults and the patients were 3.1±0.65 (mean±S.E.) per cent and 1.6±0.86 per cent, respectively. These values were obtained from the determination at 570 mμ . of wave length. CA-B quantity of the patients increased after subtotal resection of the thyroid gland and reached the almost normal level (2.7±1.14 per cent) on discharge. CA activity of the patients was slightly lower than that of normal, and positively correlated with CA-B quantity (r=0.75, P<0.001). CA-B quantity of the patients was inversely related to T3-131I resin sponge uptake value of their sera (r=-0.62, P<0.005), 131I uptake rate of the thyroid gland (r=-0.49, P<0.01) and basal metabolic rate (r=-0.55, P<0.001). Accordingly, it is considered that thyroid hormone probably reduces CA-B production, but does not inhibit CA activity in morbus basedowii, and an increase of basal metabolic rate and shortening of circulation time in the disease may be partly caused by a low level of CA-B quantity in the red cells.View full abstractDownload PDF (1118K)
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