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Volume 106 (1972)
- Issue 4 Pages 311-
- Issue 3 Pages 213-
- Issue 2 Pages 109-
- Issue 1 Pages 5-
Volume 106, Issue 2
Displaying 1-12 of 12 articles from this issue
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TSUNEO SUZUKI1972 Volume 106 Issue 2 Pages 109-123
Published: 1972
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe ultrastructural changes of heart muscle following anaphylactic shock induced by egg white injection have been studied with guinea pigs to elucidate the exact nature of the cardiac anaphylaxis. The main changes were observed in the contractile elements and they consisted of dissociation of myofibrils from intercalated discs, fragmentation of intercalated discs, lengthening of sarcomere with contraction of adjacent several sarcomeres, and fragmentation and disarrangement of myofilaments. The other changes were shrinkage or disruption of mitochondria, dilation of endoplasmic reticulum, appearance of numerous vacuoles between and inside the myofibrils, and slight increase of glycogen granules. In these changes of the heart muscle, those of the contractile elements and the mitochondria seemed to have resulted from the direct effects of anaphylactic reaction, and dilation of endoplasmic reticulum and appearance of numerous vacuoles were considered to be caused by anoxic effects of respiratory disturbance or reduction of coronary blood supply due to anaphylactic reaction. The increase of glycogen granules seemed to be caused by disturbance of oxidative enzymes in the mitochondria. These findings suggested that the effects of anaphylactic reaction on the heart muscle resulted from the direct toxic effects of anaphylactic reaction rather than the hypoxia or ischemia due to respiratory disturbance or reduction of coronary blood supply, and they seemed to support the conception that the heart should be considered to be a potential “shock organ” in the anaphylactic reaction.View full abstractDownload PDF (5952K) -
TAKESHI SHIMIZU, KAZUKUNI YAMASHITA, HAJIME SUGIHARA, HIDEO TSUCHIYAMA1972 Volume 106 Issue 2 Pages 125-134
Published: 1972
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSObservations were made in dogs to determine whether X-irradiation affects the secretion of androgens by the testes, and their responses to exogenous gonadotrophin. The secretory activity was, under pentobarbital anesthesia, determined by measuring 17-oxosteroids in spermatic vein blood. The radiation was applied with 200 or 2, 000 R only to the testes, but it failed to stimulate or significantly alter the secretion of 17-oxosteroids in a period of up to 4 hr after exposure. However, at 200 R an increased secretion of testicular 17-oxosteroids into the spermatic vein became evident within 1-6 days, whereas after 2, 000 R it was observed within 13 days only in a few cases. During the post-irradiation period of 30 days, irradiated testes responded to pregnant mare serum in doses of 40iu/kg by producing larger amounts of 17-oxosteroids than normal, the maximum response occurring within a few days following irradiation. In connection with these experiments, enzyme histochemical studies were carried out on the activity of secondary alcohol dehydrogenase in the testicular tissue. At 200 R, the enzyme activity in the Leydig cell areas was increased considerably and attained to the maximum in a faw days after irradiation, and was then progressively reduced.View full abstractDownload PDF (1454K) -
TAKASHI NAKAMURA, TOSHIMI SUZUKI, KAI TSUIKI, SHIRO TOMINAGA1972 Volume 106 Issue 2 Pages 135-145
Published: 1972
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe total limb blood flow, total muscular blood flow and effective muscular blood flow were simultaneously determined by KetySchmidt method applying hydrogen gas and hydrogen wash-out technique at rest and during exercise in 35 dogs. Applicability of continuous recording and unnecessity of blood-sampling made the method using hydrogen gas superior to others. Total blood flow (7.87±3.77ml/min/100g) was significantly greater than effective blood flow (4.73±1.90ml/min/100g) in resting dog muscle, indicating that a part of the blood circulates through the tissue non-effectively. In other words, in resting muscle, there is non-effective (non-nutritional) blood flow which passes through “functional shunt”. One of the roles played by this non-effective flow seems to be “reserve” for the increase of local needs, especially for hyperemia by mild exercise. This conclusion was reached by the fact that the increase in total muscular blood flow during mild exercise was less remarkable, while effective muscular blood flow was remarkably increased. The significant difference obtained between the total limb blood flow (10.99±4.59ml/min/100g) and the total muscular blood flow indicates possible existence of A-V-A in skin.View full abstractDownload PDF (488K) -
HIROSHI NAGURA1972 Volume 106 Issue 2 Pages 147-163
Published: 1972
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSA cell line from fibrosarcoma induced in rat by 3-methylchoranthrene was established under stationary culture conditions. The cell line (MC-1 cell line) was maintained with 70 subcultures in vitro for more than two years. The monolayer of the cell line was composed of fibroblastic cells. Electron microscopic observations revealed abundant cytoplasmic filaments and extracellular collagen fibers in the slowly growing state, but scarce fibrils in the rapidly growing state. The cells actively phagocytized extracellular proteins and particles. The amount of mucopolysaccharides on the cell membane increased significantly as compared with other cell lines investigated by ruthenium red stain method and sialic acid assay, and considerably increased in inverse proportion to diminution of growth in the MC-1 cell line.
These morphologic and functional features of the MC-1 cell line altered under some cultural conditions in proportion to its growth rate, and few truly specific signs of the establishment of a cell line except endless cell multiplication after transfers have been revealed by the present experiments.View full abstractDownload PDF (4398K) -
MASAO ENDOH, TOMOHIKO KIMURA, KOROKU HASHIMOTO1972 Volume 106 Issue 2 Pages 165-173
Published: 1972
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe relative inotropic potencies of isoproterenol, norepinephrine, epinephrine and phenylephrine were compared in the blood-perfused canine papillary muscle preparation. The muscle was perfused at a constant pressure of 100mm Hg at 38-39°C with arterial blood from a donor animal. The isometric tension developed by the papillary muscle and the positive inotropic effect of catecholamines were a function of the stimulus frequency. The potencies of the inotropic effect of catecholamines were compared at various stimulus frequencies from 90 to 240/min. Isoproterenol, norepinephrine and epinephrine, 0.001 to 0.1μg, caused reproducible positive inotropic effects lasting for from 2 to 5min, when the perfused blood flow of the muscles was from 3 to 5ml/min. Phenylephrine, 0.01 to 1μg, caused no inotropie effect. The order of potencies of inotropic effect of each amine was isoproterenol>norepinephrine>epinephrine>>phenylephrine. The dose ratio required to produce 100% increase in isometric tension was IS:NE:EP=1:3:6 at stimulus frequencies of 90 and 120/min. The ratio of the percentage increase produced by 0.03μg of each agent was roughly IS:NE:EP=3:2:1 and 5:3:1 at 90 and 120/min, respectively. Norepinephrine produced a distinctly stronger positive inotropic effect than did epinephrine and the differences between these amines were statistically significant.View full abstractDownload PDF (1518K) -
TAKASHI KITABATAKE, RYO KAYAMORI, MASAO HARA, HIROSHI HINATA, EIJI KAS ...1972 Volume 106 Issue 2 Pages 175-183
Published: 1972
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSA method of taking transverse section scintigram, using a rotating cathode ray tube with a sweep circuit, is described. A rotating table for a patient is prepared, which is set so as to be that the rotation center of a rotating table should be placed within the perpendicular line from the center of the detector facing sideways. A rotating table rotates synchronously with rotation of the deflection coil of CRT. Two points 2.5 cm apart from each other could narrowly resolved in a transverse section scintigram thus obtained. This method has some characteristics differ from those have already been reported by others.View full abstractDownload PDF (2134K) -
OKIHIKO SHIBATA, SHINJI NARITA, KINYA EGASHIRA, SHINJI WAKI, TATUZI SU ...1972 Volume 106 Issue 2 Pages 185-189
Published: 1972
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSHypophysectomized dogs were used to study the effect of hemorrhage-induced hypotension upon the responsiveness of the adrenal cortex to adrenocorticotrophin (ACTH). Adrenal venous blood samples were collected under sodium pentobarbital anesthesia and were analyzed for 17-hydroxycorticosteroids (17-OHCS). ACTH was infused intravenously throughout each experiment. A sustained rise in adrenal 17-OHCS secretion was produced. Marked falls in arterial blood pressure and adrenal blood flow were induced by a removal of 20-25ml/kg of arterial blood. The rate of adrenal 17-OHCS secretion was decreased to the level of 28-65% of the pre-hemorrhage secretion rate.View full abstractDownload PDF (318K) -
KAORU SAGISAKA, KAZUO TOKIWA, NAOHUMI YOSHIOKA1972 Volume 106 Issue 2 Pages 191-197
Published: 1972
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe agglutinability of the proteolytic enzyme treated M, N and MN red cells with any one of the anti-M and -N antisera, eluted antibodies and class antibodies was studied. The enzyme-treated 141 and N red cells lost their group specificity in agglutination with the absorbed antisera. However, it was noted that the enzyme-treated M red cells acquired N agglutinogen instead of losing their own M agglutinogen in agglutination with the eluted antibodies. The enzyme-treated N red cells maintained the group specificity. Agglutination of the enzyme-treated red cells with the class antibodies showed clearly that the changes in agglutinability of the red cells were caused by each of the class antibodies.View full abstractDownload PDF (388K) -
MASAO TANAKA, HIROSHI OHTA1972 Volume 106 Issue 2 Pages 199-206
Published: 1972
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSA case of primary acquired sideroblastic anemia is described, in whom erythroblasts showed markedly reduced δ-aminolevulinic acid synthetase activity. The patient, 68-year-old Japanese female, was admitted with a history of anemia and congestive heart failure of 3 year duration. By hematological study, marked hypochromic anemia without apparent morphologic aberration was noted. Examination of bone marrow disclosed the intensive erythroid hyperplasia with minor megaloblastoid changes and maturation arrest. Iron stores in bone marrow were greatly increased with predominating sideroblasts. Serum iron was elevated and ferrokinetic study indicated normal rate of iron clearance and depressed red cell iron utilization. In the studies on porphyrin metabolism, incorporation of 14C-glycine into heme and activity of S-aminolevulinic acid (ALA) synthetase were found to be greatly reduced. Although there have been several reports on the defects in the heme synthetic pathway in sideroblastic anemia, this is a first case of sideroblastic anemia with deficient ALA synthetase activity demonstrated by a direct method.View full abstractDownload PDF (1179K) -
AKINORI NISHIYAMA, AKIRA RIKIMARU, YASUE FUKUSHI, TAIZO SUZUKI1972 Volume 106 Issue 2 Pages 207-208
Published: 1972
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSSubstance R, a newly extracted biologically active substance, had some hypotensive effect on the cat mean arterial blood pressure. This substance, however, neither decelerated nor accelerated the heart rhythm. The hypotensive effect of this active substance may therefore be due to the vasodilatatory effect of this substance.View full abstractDownload PDF (67K) -
YOSHIRO FUKADA, HIDEAKI SAITO1972 Volume 106 Issue 2 Pages 209-210
Published: 1972
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSRelay neurons in the cat's lateral geniculate nucleus have either on-center or off-center receptive field. They are further classified into Type-I (phasic) and Type-II (tonic). Type-I cells are activated by faster conducting opfic tract fibers and send the visual information to visual cortex by faster conducting radiation fibers, while Type-II cells area ctivated by slower fibers and extend slower radiation fibers, while Type-II cells are activated by slower fibers and extend slower radiation fibers, while Type-II cells are activated by slower fibers and extend slower radiation fibers.View full abstractDownload PDF (120K) -
RYOICHI SHIRACHI, NORIYOSHI SUKENO, TAKESHI SASAKI, TAKUSEI UMENAI, TA ...1972 Volume 106 Issue 2 Pages 211-212
Published: 1972
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe incidence of Australia (Au) antigen among male (1.9%) was higher than that among female (1.4%) in Miyagi Prefecture. In three counties of the Prefecture, higher incidence of Au antigen than that among general population was noticed.View full abstractDownload PDF (134K)
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