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Volume 152 (1987)
- Issue 4 Pages 319-
- Issue 3 Pages 211-
- Issue 2 Pages 103-
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Volume 152, Issue 3
Displaying 1-13 of 13 articles from this issue
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AKIKO YAMAMOTO, OSAMU WADA, HIRAMITSU SUZUKI1987 Volume 152 Issue 3 Pages 211-219
Published: 1987
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSYAMAMOTO A., WADA O. and SUZUKI H. Separation of Biologically Active Chromium Complex from Cow Colostrum. Tohoku J. exp. Med., 1987, 152 (3), 211-219-By a procedure based on ion-exchange chromatography, five chromium-containing fractions were separated from the ethanol extract (50-90%) of cows' colostrum which should provide infant with all or most of the chromium needed in available form. Three of the fractions are anionic (A-1, A-2 and A-3) and two are cationic (C-1 and C-2). Two major fractions, A-1 and A-3, comprise together about 77% of the total chromium recovered in the effluent after ion- exchange chromatography and C-1 and C-2 comprise together only about 21%. Approximate molecular weight was determined by the Sephadex gel chromatography to be 1, 500 for A-1 and C-2 and 2, 000 for C-1. A-3 was eluted from the Sephadex gel column at the elution position corresponding to that of inorganic trivalent chromium. Among five fractions, A-1 shows glucose tolerance factor (GTF) activity as measured by the stimulation of [U-14C] glucose oxidation to 14CO2 in rat epididymal adipocytes. A-1 stimulates glucose oxidation at chromium concentration as low as 100pg/ml only when it is incubated with insulin. These findings suggest that A-1 fraction separated from cow milk contains a low-molecular-weight, chromium complex which plays a role in glucose metabolism in close relation with the action of insulin.View full abstractDownload PDF (849K) -
NORIKO AKAZAWA, SHIN-ICHI MIKAMI, SHU-ICHI KIMURA1987 Volume 152 Issue 3 Pages 221-229
Published: 1987
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSAKAZAWA, N., MIKAMI, S. and KIMURA, S. Effects of Vitamin E Deficiency on the Hormone Secretion of the Pituitary-Gonadal Axis of the Rat. Tohoku J. exp. Med., 1987, 152 (3), 221-229 Chronological changes of gonadotropin (FSH and LH) and testosterone concentrations in the serum were measured in vitamin E deficient rats to investigate the effects of vitamin E deficiency on the pituitary-gonadal function in rats. The receptor sites and association constant (Ka) for LH and the formation of cyclic AMP in the Leydig cells were also investigated. The results obtained in the present study are as follows: 1) The vitamin E deficient rats showed almost complete hemolysis and extremely increased TBA reacting substances (TBARS) in the serum and liver. 2) The serum LH concentration in the vitamin E deficient group was slightly higher than in the vitamin E supplemented group during the later periods of experiment. 3) The serum FSH concentration in the vitamin E deficient group did not differ significantly from that in vitamin E supplemented group, but became significantly higher than that in the latter at 186 days of experiment. 4) The serum testosterone concentration was always lower in the vitamin E deficient group than in the control. 5) The vitamin E deficient group showed slightly large number of LH/hCG receptor and significantly small Ka (low affinity), as compared with vitamin E supplemented group. The formation of cyclic AMP by Leydig cells decreased significantly in vitamin E deficient group. These results suggest that the vitamin E deficiency exerted a suppressive effect directly on the gonadal function to decrease the hormone synthesis in the Leydig cells and caused the increased secretion of pituitary LH owing to the feedback mechanism.View full abstractDownload PDF (884K) -
MITSURU KONN, MUTSUO SASAKI, YUJI YAMANAKA, TAKAYUKI MORITA, KEIICHI O ...1987 Volume 152 Issue 3 Pages 231-235
Published: 1987
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKONN, M., SASAKI, M., YAMANAKA, Y., MORITA, T. and ONO, K. Interferon Producing Activity of Lymphnode and Peripheral Blood Lymphocytes in Cancer Patients. Tohoku J. exp. Med., 1987, 152 (3), 231-235-The IFN producing activity of RLNL and PBL was studied in cancer patients using K562, PHA-P and OK-432 as inducers. In response to K562, IFN production was significantly greater in the PBL than in the RLNL, and the titer was about equal in the PBL and RLNL when OK-432 was used as an inducer. On the contrary, the amount of IFN produced by PHA-P was significantly higher in the RLNL than in the PBL. There was no definite relationship recognized between tumor progression and IFN productivity in the cases studied.View full abstractDownload PDF (318K) -
ROKO KUBOTA, KAZUO KUBOTA, TAIJU MATSUZAWA, SHUMPEI TAKEDA1987 Volume 152 Issue 3 Pages 237-245
Published: 1987
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKUBOTA, R., KUBOTA, K., MATSUZAWA, T. and TAKEDA, S. Metastatic Enhancement and Growth Inhibition by Glucocorticoid and Its Receptors in Mouse Teratocarcinoma OTT6050 in Vivo. Tohoku J. exp. Med., 1987, 152 (3), 237-245 -To elucidate the mechanism of glucocorticoid-induced metastases and growth inhibition of mouse teratocarcinoma OTT6050, syngeneic 129/Sv-CP+SlJ mice were subcutaneously (s.c.) injected with the tumor and treated with daily intraper-itoneal (i.p.) injections of hydrocortisone in doses of 1.5 to 12.5mg per mouse. The number of lymphnode and visceral metastases was greatly increased by the treatment with hydrocortisone unrelated to the doses and sexes. Tumor growth (the products of three principal diameters) of lymphnode metastases was dose-dependently inhibited by the treatment. Histologically, lymphnode metastatic tumors contained large masses of well-differentiated tissues compared with the s.c. transplanted tumor of the same size. The glucocorticoid receptors were measured using dextran-coated charcoal adsorption techniques with [3H] hydrocortisone. Glucocorticoid receptor contents were higher in lymphnode metastatic tumor (100 fmole/mg cytoplasmic protein, mean) than in s.c. transplanted tumors (74.5fmole/mg, mean) respectively treated with 12.5mg of hydrocortisone. The metastatic growth inhibition by hydrocortisone might be closely related to the receptor-mediated mechanisms and histological differentiation.View full abstractDownload PDF (813K) -
AKIHIRO FUNAKOSHI, DAVID E. SCHTEINGART, AARON I. VINIK1987 Volume 152 Issue 3 Pages 247-251
Published: 1987
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSFUNAKOSHI, A., SCHTEINGART, D.E. and VINIK, A.I. Effect of Insulin on Motilin Release in Man. Tohoku J. exp. Med., 1987, 152 (3), 247-251 The effect of insulin on motilin release was investigated by use of the euglycemic glucose clamp technique. By use of this technique plasma glucose concentration was maintained constant at 80-90mg/100ml, and plasma insulin immunoreactivity (IRI) was increased from 15±6μU/ml to 171±22μU/ml in 10min, and remained at this level for 2hr. Plasma motilin like immunoreactivity (MLI) concentration decreased within 10min from 199±36pg/ml to 120±28pg/ml and remained low during the course of study. A significant negative correlation between MLI and IRI concentrations (r=-0.72, p<0.01) was observed. The present results indicate that the suppressive effect of insulin on motilin release is a direct action of insulin and is not mediated by glucose.View full abstractDownload PDF (493K) -
TAI MURYOI, TAKESHI SASAKI, EIICHI TAMATE, OSAMU TAKAI, NAOKO HARATA, ...1987 Volume 152 Issue 3 Pages 253-258
Published: 1987
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSMURYOI, T., SASAKI, T., TAMATE, E., TAKAI, O., HARATA, N. and YOSHINAGA, K. Antigen Inhibition of the Interaction between Murine Monoclonal Anti-Idiotypic Antibodies and Human Monoclonal Anti-DNA Antibodies. Tohoku J. exp. Med., 1987, 152 (3), 253-258-Monoclonal anti-idiotypic (Id) antibodies to human monoclonal anti-DNA antibodies were obtained by a somatic cell hybridization. One, termed as D1E2, was directed to Id of anti-single-stranded (ss) DNA antibody (0-81) and the other, 1F5, to anti-double-stranded (ds) DNA antibody (NE-1). Each anti-Id antibody behaved like a mirror image of the corresponding antigens, when determined by competitive inhibition radioimmunoassay. Therefore, D1E2 and 1F5 are regarded as Ab2β or Ab2γ. These antibodies will make useful reagents to understand and manipulate the autoantibody production in human.View full abstractDownload PDF (551K) -
KOICHI NAKAYAMA1987 Volume 152 Issue 3 Pages 259-267
Published: 1987
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSNAKAYAMA, K. On-Line Recording of Vascular Resistance in Isolated Canine Renal Artery by Use of a Differential Pressure Transducer. Tohoku J. exp. Med., 1987, 152 (3), 259-267-For the assessment of the validity of the perfusion system of vascular segments, both isolated canine renal artery in cylindrical form and artificial tubing were perfused intra- and extraluminally, and vascular resistance was continuously recorded by use of a differential pressure transducer. Themodel experiments using artificial tubing showed that the vascular resistance, inversely related to outflow and differential pressure, enormously increased when the lumen was occluded more than 85% of the control. Various agonistic stimuli such as norepinephrine, phenylephrine, methoxamine, prostaglandin F2α and 5-hydroxytryptamine, when administered intraluminally, increased the vascular resistance, while verapamil and papaverine decreased it. Transmural electricalstimulation also increased the resistance which was susceptible to tetrodotoxin. The removal of endothelium by infusion of saponin augmented the contractile responses to agonistic stimuli such as norepinephrine and 5-hydroxytryptamine. The results indicate that the present perfusion method seems to be a good alternative for studying contractile activity and endothelium function of isolated vascular tissues.View full abstractDownload PDF (823K) -
KAZURO KAISE, NOBUKO KAISE, KATZUMI YOSHIDA, YOICHI ITAGAKI, MAKIKO YA ...1987 Volume 152 Issue 3 Pages 269-275
Published: 1987
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKAISE, K., KAISE, N., YOSHIDA, K., ITAGAKI, Y., YAMAMOTO, M., SAKURADA, T., SAITO, S., and YOSHINAGA, K. Characteristics of Anti-TSH Receptor Antibodies in Two Patients Who Developed Spontaneous Hypothyroidism after Antithyroid Drug Therapy for Hyperthyroid Graves' Disease. Tohoku J. exp. Med., 1987, 152 (3), 269-275-Spontaneous hypothyroidism was developed in two patients with Graves' hyperthyroidism 1.8 or 1 years after antithyroid drug therapy. In these patients anti-thyrotropin (TSH) receptor antibodies were detected. Thyrotropin receptor antibody (TRAb) index measured with TSH radioreceptor assay was 61.0% in case 1 and 68.0% in case 2 (normal value: -10 to 10%), and human thyroid adenylate cyclase stimulating activities (HTACS) were 154% in case 1 and 190% in case 2 (normal value: 70 to 130%). IgG from case 1 suppressed the stimulating activity of bovine TSH (bTSH) on thyroid adenylate cyclase in low concentrations in vitro. These results indicate that a blocking type of anti-TSH receptor antibodies as well as concomitant chronic thyroiditis might be a causative factor in patients who developed spontaneous hypothyroidism shortly after the cessation of antithyroid drugs.View full abstractDownload PDF (624K) -
YASUHIRO AOKI, MASATO FUNAYAMA, KAORU SAGISAKA1987 Volume 152 Issue 3 Pages 277-281
Published: 1987
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSAOKI, Y., FUNAYAMA, M. and SAGISAKA, K. Detection of AB Antigen in Blood Stain Using Enzyme-Linked Immunosorbent Assay. Tohoku J. exp. Med., 1987, 152 (3), 277-281-A new method for detection of AB antigen from blood stain using enzyme-linked immunosorbent assay (ELISA) is described. Flat-bottomed-wells of polystylen plate coated with human anti-B or rabbit anti-A were sensitized with AB antigen which was extracted from blood stain with 1% octyl-glucopyranoside in 0.1M phosphate buffer pH 8.0. Mouse monoclonal anti-A or anti-B, and peroxidase conjugated anti-mouse immunoglobulin were added to the wells, respectively. Subsequently, the substrate was dropped into the wells, and the absorbance of the solution was measured. By this method, we could distinguish AB group blood stain from the mixed stain of A and B group bloods. When rabbit antiserum was used as the first antibody, differentiation between these antigens was unsuccessful presumably because of non-specific adsorption.View full abstractDownload PDF (397K) -
MUNEYA SUZUKI, NORIHIKO TERAO, YASUSHI ASANO, SAICHI HOSODA1987 Volume 152 Issue 3 Pages 283-290
Published: 1987
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSUZUKI, M., TERAO, N., ASANO, Y. & HOSODA, S. Adaptive Changes of H+ and/or Bicarbonate Transport in Chronic Metabolic Acidosis: Its Quantitative Evaluation by Using Isolated Perfused Kidney. Tohoku J. exp. Med., 1987, 152 (3), 283-290-We have recently improved the technique of the isolated perfused rat kidney by addition of amino acids to the perfusion solution. Utilizing this improved preparation, we examined to what extent chronic acid-base status can alter H+ and/or bicarbonate transport in the whole kidney and the effect of acetazolamide (10-4M) on this process. Kidneys from rats that had been drinking tap water (control), 1.5% NH4Cl (acidotic), or 1.5% NaHCO3 (alkalotic) were perfused with a solution of identical pH and PCO2. During the control period, the fractional bicarbonate reabsorption was higher in acidotic rats than in control rats by 1.0% and urine pH was lower than those in control. On the other hand, the two parameters were not different between control and alkalotic rats. Acetazolamide increased urine pH and decreased the fractional bicarbonate reabsorption in all, but these parameters in acidotic and alkalotic rats were not different compared with those in control. We conclude that adaptive changes of H+ and/ or bicarbonate transport in chronic metabolic acidosis corresponds to about 1.0% as fractional bicarbonate reabsorption by the isolated perfused kidney and is largely carbonic anhydrase dependent.View full abstractDownload PDF (670K) -
MITSURO CHIBA, OSAMU MASAMUNE, YUTAKA YOSHIDA, SHINOBU KAROUJI1987 Volume 152 Issue 3 Pages 291-300
Published: 1987
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSCHIBA, M., MASAMUNE, O., YOSHIDA, Y. and KAROUJI, S. NK Activities and NHK-1+ Cells in Familial Polyposis Coli. Tohoku J. exp. Med., 1987, 152 (3), 291-300-Although natural killer cells are thought to play a major role in surveillance against tumor development, no systemic studies on natural killer cells and their activities have been reported in familial polyposis coli in which cancer always develops at an early age. Natural killer activities and natural killer cells were studied in 14 patients with familial polyposis coli. Peripheral blood lymphocytes from familial polyposis coli showed normal cytotoxicities against K562 cells, P4788 cells derived from colon cancer and Chang cells. The number of natural killer cells in peripheral blood lymphocytes identified by an immunofluorescence antibody method and that in gut tissue by immunoperoxidase staining using anti-HNK-1 monoclonal antibody were comparable to those seen in normal controls. These results led to the following conclusion: it seems unlikely that a deficiency or impairment of natural killer activity is one of backgrounds for the development and subsequent malignant change of adenomas in familial polyposis coli.View full abstractDownload PDF (3003K) -
NOBORU NUSHIRO, KEISHI ABE, MASAHIDE SEINO, SADAYOSHI ITOH, KAORU YOSH ...1987 Volume 152 Issue 3 Pages 301-310
Published: 1987
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSNUSHIRO, N., ABE, K., SEINO, M., ITOH, S. and YOSHINAGA, K. The Effects of Atrial Natriuretic Peptide on Renal Function and the Renin-Aldosterone System in Anesthetized Rabbits. Tohoku J. exp. Med., 1987, 152 (3), 301-310 To determine the effects of α-human atrial natriuretic peptide (ANP) on renal function and the renin-aldosterone system in anesthetized rabbits, ANP (0.05μg/kg/min) or 5% dextrose solution in vehicle control was infused intravenously. The infusion ofANP resulted in a significant decrease in mean arterial pressure with an increase in renal blood flow (RBF). ANP also produced significant increases in urine volume and urinary sodium excretion. ANP tended to increase glomerular filtration rate, filtered sodium load and net tubular reabsorption of sodium. However, there were no significant differences in these parameters compared with control group. Fractional sodium excretion was increased significantly by ANP. Plasma renin activity (PRA) was suppressed only at 30min after the infusion of ANP, while a significant fall in plasma aldosterone concentration (PAC) lasted even in the recovery period. These results indicate that ANP produces a diuresis and natriuresis through the increased RBF in anesthetized rabbits. It is also suggested that ANP suppresses PAC independent of the inhibition of PRA.View full abstractDownload PDF (949K) -
MOTOAKI YOSHIDA, KANJI IZUMI, TAKAO NAKANISHI1987 Volume 152 Issue 3 Pages 311-317
Published: 1987
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSYOSHIDA, M., IZUMI, K. and NAKANISHI, T. Effects of Thyrotropin Releasing Hormone (TRH) on Glutamate-Induced Seizures in Rats. Tohoku J. exp. Med., 1987, 152 (3), 311-317-We investigated effects of TRH on glutamate-induced seizures in rats. Rats were injected intraperitoneally with sodium glutamate in a dose (22.3mmole/kg) equivalent to ED95 for generalized convulsions. Twenty five min later TRH in a dose of maximum effect (40μg in the tartrate form) was injected intracerebroventricularly in order that the time of peak effect was simultaneous with the onset of seizure which was anticipated from the preliminary study. TRH delayed the onset of initial seizures significantly, though neither occurrence nor incidence of various seizure activities was affected. A trend of delay in the mean time of death was also observed. These results suggest that TRH has an inhibitory action against glutamate-induced seizures in rats, albeit mild.View full abstractDownload PDF (685K)
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