Kaketa fractions
1), obtained by a modification of Nakagawa and Kasai procedure
2), of united cancerous urines with original seat in stomach, liver, lung or mamma and of normal urine were employed separately. The material was dissolved in slightly alkaline water and precipitated by acidulation with acetic acid. The precipitate was liberated from most of an urinary poison by treatment with Dowex 1 and electrodialysis, and the deposit occurring on the electrodialysis was added to a column of Dowex I in borate form and chromatographed with the same buffer to isolate the main component in an electrophoretically homogeneous state. Average yields of the cancerous and normal products amounted to 50 and 34mg. per liter of urine respectively. Those products were both soluble in water at other pH values than 4.4-4.6, and free from phosphorus and proved to be glucidamins. The former product: N 13.5%, hexosamine 3.0%, galactose 1.6%, glucose 2.0%, mannose 1.5%, L-fucose 0.4%, sialic acid 3.4%, ash 0%, [α]
7D=-110°; the latter product: N 13.8%, hexosamine 2.9%, galactose 1.9%, glucose 1.0%, mannose 1.4%, L-fucose 0.5%, sialic acid 4.4%, ash 0.6%, [α]
7D=-100°. They proved both to be toxohormones, with the minimum active dosis per mouse of 1mg. in the former and with that of 5mg. in the latter. They lack KIK- and group-potency.
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