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Volume 162 (1990)
- Issue 4 Pages 293-
- Issue 3 Pages 203-
- Issue 2 Pages 109-
- Issue 1 Pages 1-
Volume 162, Issue 2
Displaying 1-9 of 9 articles from this issue
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SHIGEO YAMAKI1990 Volume 162 Issue 2 Pages 109-119
Published: 1990
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSYAMAKI, S. Pulmonary Vascular Disease in Shunted and Nonshunted Patients with Tetralogy of Fallot. Tohoku J. Exp. Med., 1990, 162 (2), 109-119 - Histometric analysis of pulmonary vascular disease was performed in 13 shunted and 21 nonshunted patients with tetralogy of Fallot, and in 29 normal controls. There was no significant difference in the medial thickness of the small pulmonary arteries between cases of tetralogy of Fallot and normal controls, but the media in shunted cases of tetralogy of Fallot were thicker than in the nonshunted cases. Intimal fibrosis, regarded as organized thrombi, and thrombi of small pulmonary arteries were observed generally after 4 years of age in both the shunted and nonshunted cases of tetralogy of Fallot. Intimal proliferation of musculoelastosis which was formed of longitudinal smooth muscle bundles and elastic fibers was characteristic in shunted patients, especially after the central palliation procedure, Waterston anastomosis or modified Blalock-Taussig (BT) anastomosis using the Gore-Tex tube graft. However, it was not usually seen in the BT anastomosis cases. Unexpected pulmonary hypertension due to the shunt operation is thought to be the cause of musculoelastosis, because musculoelastosis was observed even in a patient with pulmonary hypertension only 3 weeks following surgery. We therefore recommend the original BT anastomosis as the shunt procedure. When considering the use of other shunt operations in which there is a possibility of placing a pressure load on the pulmonary vascular bed, attention must be given to the size of the anastomosis or artificial tube graft.View full abstractDownload PDF (2421K) -
VINOD P. GAUR, KATSUHIRO YAMAGUCHI, JAMES E. TURNER1990 Volume 162 Issue 2 Pages 121-126
Published: 1990
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSGAUR, V. P., YAMAGUCHI, K. and TURNER, J.E. Somatostatin in Rat Retina: Localization by In Situ Hybridization Histochemistry and Immunocytochemistry. Tohoku J. Exp. Med., 1990, 162 (2), 121-126 - In order to analyse the mRNA-protein relationships of somatostatin containing neurons in the rat retina, we have used a combined method with in situ hybridization histochemistry using radio-labeled oligodeoxyribonucleotides complementary for rat somatostatin mRNA and immunocytochemistry using antiserum for somatostatin protein. We found double labeled amacrine cells in the innermost and outermost laminae of the inner nuclear layer as well as the displaced amacrine cells in the ganglion cell layer in the retina. Some cells in the inner nuclear layer had high levels of somatostatin mRNA. Radially immunostained interplexiform fibers were observed adjacent to the amacrine cells with high level of somatostatin mRNA. These data suggest the site of synthesis of somatostatin and post-translational migration of this neuropeptide within the rat retinal tissueView full abstractDownload PDF (1564K) -
YOSHIAKI KANO, TOMOKUNI TANIGUCHI, YAHIRO UEMURA, KAZUMASA YOKOYAMA, H ...1990 Volume 162 Issue 2 Pages 127-136
Published: 1990
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKANO, Y., TANIGUCHI, T., UEMURA, Y., YOKOYAMA, K., OHYANAGI, H. and SAITOH, Y. Selection of Monoclonal Antibodies Detecting KM01 Antigen. Tohoku J. Exp. Med., 1990, 162 (2), 127-136 - Monoclonal antibodies (MoAbs) were selected for specific binding inhibition of KM01 to the KM01 antigen, which was detected in the serum of colorectal and other gastrointestinal carcinoma patients. Out of 91 MoAbs tested, 8 were found to have the inhibiting activity. The reactivity of these MoAbs with glycolipid antigen, which was isolated from cancer cell line, corresponded well with that of cancer patients sera. These monoclonal antibodies are thought to be useful in combination with KM01 for the construction of the second generation of the KM01 antigen-detecting system.View full abstractDownload PDF (1393K) -
TAKAKO OKAYASU, KAZUE SHIGIHARA, NORIO KOBAYASHI, AKASHI ISHIKAWA, NAO ...1990 Volume 162 Issue 2 Pages 137-145
Published: 1990
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSOKAYASU, T., SHIGIHARA, K., KOBAYASHI, N., ISHIKAWA, A., FUKUSHIMA, N., TAKASE, A., HATTORI, S., NAKAJIMA, T., SHISHIDO, T. and AGATSUMA, Y. A Family Case of Nephroqenic Diabetes Insipidus. Tohoku J. Exp. Med., 1990, 162 (2), 137-145 - Two brothers, patient 1 with fever and vomiting, and patient 2 with failure to gain weight were studied. After 4hr of water deprivation test. the urinary osmolality of the patient 1 was only 105 mOsm/liter and his body weight showed a 4.6% reduction. In response to desamino-8-D arginine vasopressin intranasal administration, no significant elevation of urinary osmolality of patient 1 occurred. After low dose vasopressin tests, the maximal urinary osmolality of their father was in the normal range, but that of their mother was below the normal range. Moreover, the patients showed no significant increase of urinary osmolality after the same tests. The brothers were diagnosed as nephrogenic diabetes insipidus (NDI) and their mother was diagnosed as a carrier. An early diagnosis of NDI is important, since adequate managements such as low-solute diet with restricted protein and salt intake or such as water intake at frequent intervals can prevent the hyperosmolality which would develop the delayed mental and physical developments. The usefulness of the combination of indomethacin with thiazide diuretics is described.View full abstractDownload PDF (816K) -
AKIMITSU MIYAUCHI, CHIHARU KIRAMINE, SATOSHI TANAKA, KENJI HOJO1990 Volume 162 Issue 2 Pages 147-167
Published: 1990
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSMIYAUCHI, A., HIRAMINE, C., TANAKA, S. and HOJO, K. Differential Effects of a Single Dose of Cyclophosphamide on T Cell Subsets of the Thymus and Spleen in Mice: Flow Cytoluorometry Analysis. Tohoku J. Exp. Med., 1990, 162 (2), 147-167 - Sequential changes in the distribution of lymphocyte subpopulations of the thymus and spleen in BALB/c mice (male, 8-weeks old) during 2 weeks after a single i.p, injection of cyclophosphamide (CY, 200mg/kg body weight) were studied mainly through the use of CD4, CD8 and CD3-ε markers together with single- or two-color flow cytofluorometry. In the thymus on Day 2 after CY treatment, a marked decrease in the number and proportion of PNAhi, CD3- and CD4+CD8+ double positive (DP) subpopulation was observed in parallel with a marked reduction in the thymus weight, cortical area and total thymocyte number. This phenomenon might be associated with the decrease in the percentage of thymocytes at the S phase of the cell cycle on Day 1 and 2 after CY treatment, owing to the depletion of the rapidly dividing cells in DP subset. There was a significant reduction in the number and proportion in CD4+ single positive(SP) subset on Day 7. The cell number of CD8+SP subset continued to decrease during Day 7 to Day 14. This contrasted with the behaviors of DP, CD4+SP and CD4- CD8- double negative (DN) subsets in which a considerable recovery was attained by Day 14. The spleen from CY-treated mice showed a marked decrease in the DN subset and surface immunoglobulin-positive cells, perhaps B lymphocytes, in both the percentage and the absolute cell number on Days 2 and 7, which paralleled the marked reduction in its weight and total cell number. The absolute cell numbers of CD4+SP and CD8+SP subsets in the spleen were also reduced on Days 2 and 7. The reduction of the CD4+SP/CD8+SP ratio was found in the thymocytes on Days 2 and 7 but not in spleen cells. Our results suggest that the principal target cell population of CY is the PNAhi, CD3- and DP immature cortical thymocytes as well as splenic B cells. The sustained decrease in the number of CD8+SP thymocytes after CY treatment might be in part relevant to the potentiating effect of CY pretreatment on immune responses.View full abstractDownload PDF (2957K) -
KATSUYA YAMAMOTO, TAKESHI KOHNOSU, KAN-ICHI NAKAGAWARA, KYOKO SAKAI1990 Volume 162 Issue 2 Pages 169-175
Published: 1990
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSYAMAMOTO, K., KOHNOSU, T., NAKAGAWARA, K. and SAKAI, K. Effects of Antiepileptic Drugs and Organic Brain Damage on SCE Frequency in Cerebral Palsies. Tohoku J. Exp. Med., 1990, 162 (2), 169-175 - In order to assess the genotoxic effects of antiepileptic drugs (AEDs), analysis of sister chromatid exchanges (SCEs) was performed in lymphocytes of three groups of males; epileptics with AED therapy, non-epileptics without AED therapy and healthy controls. The epileptics and non-epileptics were cases of severe cerebral palsies due to perinatal asphyxia. Possible confounding factors of SCE frequencies, such as age, smoking habit, drug usage other than AED, recent history of viral infection, etc. were controlled. The frequency of SCE per cell was 4.63±0.71 (mean±S.D.) in epileptics, 4.70±0.89 in non-epileptics and 3.84±0.56 in healthy controls. SCEs in both epileptics and non-epileptics were significantly higher (p<0.05) than those in controls. There was no significant difference of SCE frequency between epileptics and non-epileptics. These results suggested that no mutagenic effect of AED could be demonstrated as revealed by SCEs, and organic brain damage per se might influence the baseline SCE frequency. The possible explanations for such observations are discussed.View full abstractDownload PDF (679K) -
KAZUO IRITA, HIRONORI SAKAI, HIDEHIKO ISOBE, MASAE YAMAKAWA, HAJIME NA ...1990 Volume 162 Issue 2 Pages 177-182
Published: 1990
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSIRITA, K., SAKAI, H., ISOBE, H., YAMAKAWA, M., NAWATA, H. and YOSHITAKE, J. The Effect of OP 2507, a Stable Analogue of Prostacyclin, on Hep G2 Exposed to Hypoxia. Tohoku J. Exp. Med., 1990, 162 (2), 177-182 - We developed a model for screening drugs to reduce ischemic liver damage using Hep G2, a hepatoblastoma cell line, and examined the effect of OP 2507, a stable analogue of prostacyclin, on hypoxic cell damage. Hypoxic exposure of the cells was done for 16hr in an air-tight chamber which was placed inside an incubator and was purged with 5% CO2/95% N2. Adding OP 2507 (0.01-10ng/ml) to the incubation medium during hypoxic exposure reduced mitochondrial damage estimated by MTT-reducing activity, while it failed to inhibit lactate accumulation in the medium. OP 2507 seems to be a good candidate for improving the preservation of liver allografts.View full abstractDownload PDF (644K) -
KAZUO MORI, HIDEAKI SAKAI, SOZO SUZUKI, YASUYUKI AKUTSU, MASAAKI ISHIK ...1990 Volume 162 Issue 2 Pages 183-193
Published: 1990
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSMORI, K., SAKAI, H., SUZUKI, S., AKUTSU, Y., ISHIKAWA, M., IMAIZUMI, M., TADA, K., AIHARA, M., SAWADA, Y., YOKOYAMA, M., SATO, Y., ENDO, Y., SUZUKI, Z., SATO, S., SASAKI, H., YOKOYAMA, S., HAYASHI, T., UCHIDA, T., HIWATASHI, K., ISHIDA, N., FUZIMAKI, M. and YAMADA, K. Effects of Glycyrrhizin (SNMC: Stronger Neo-Minophagen C®) in Hemophilia Patients with HIV-1 Infection. Tohoku J. Exp. Med., 1990, 162 (2), 183-193 - Forty-two hemophiliacs with HIV infection were treated with high-dose glycyrrhizin, Stronger Neo-Minophagen C® (SNMC). The dose was 100-200ml of SNMC in 21 patients and 400-800ml in the other 21. The patients were divided into an asymptomatic carrier (AC) group and AIDS related-complex (ARC) AIDS group. SNMC was administered intravenously daily for the first 3 weeks, and every second day for the following 8 weeks to the 42 HIV-infected hemophilia patients, in accordance with the protocol proposed by the Japanese National Research Committee. The CD4/CD8 ratio and CD4 positive lymphocyte counts did not change during the treatment period. However, significant improvement was noted in some cases. A slight increase in mitogenic responsiveness to phytohemagglutinin, Concanavalin A and pokeweed mitogen was noted in most patients of both groups, especially significant improvement was seen in the AC group administered over 400ml of SNMC. Furthermore, complete improvement was noted in liver dysfunction, which has been thought to be one of the major problems for hemophiliacs treated with blood products. Thus, prophylactic administration of high-dose SNMC to HIV positive hemophiliacs who have impaired immunological ability and liver dysfunction was considered to be effective in preventing the development from AC/ARC to AIDS.View full abstractDownload PDF (818K) -
YUSAKU TAZAWA, SHUNZO HAYAMIZU, KEIKO TAMAOKI, RYO CHIBA1990 Volume 162 Issue 2 Pages 195-201
Published: 1990
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSTAZAWA, Y., HAYAMIZU, S., TAMAOKI, K. and CHIBA, R. Subclinical Liver Dysfunction in One-Month-Old Infants with a Low Activity of Vitamin K Dependent Coagulant Factors Assessed by Normotest. Tohoku J. Exp. Med., 1990, 162 (2), 195-201 - To clarify features of late vitamin K deficiency hemorrhagic disease in Japanese infants, seventeen of 1, 687 infants screened by normotests were examined for signs and symptoms suggesting hepatobiliary diseases. Clinical observations disclosed findings suggesting hepatobiliary diseases in 7 of the 17 selected infants with normotest values of less than 40%, and 11 infants had abnormal results in one or more liver function tests. Taken together, 14 of the 17 infants had findings suggesting hepatobiliary diseases. Upon vitamin K supplementation normotest values improved in various degrees in all infants, whether or not they had signs or symptoms of hepatobiliary diseases. Late vitamin K deficiency hemorrhagic disease of infancy may be related to subclinical hepatobiliary diseases.View full abstractDownload PDF (581K)
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