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Volume 192 (2000)
- Issue 4 Pages 239-
- Issue 3 Pages 165-
- Issue 2 Pages 99-
- Issue 1 Pages 1-
Volume 192, Issue 4
December
Displaying 1-7 of 7 articles from this issue
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Regular Contributions
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Akiyoshi Tanaka, Tomio Ueno, Masaaki Oka, Takashi Suzuki2000 Volume 192 Issue 4 Pages 239-247
Published: 2000
Released on J-STAGE: March 29, 2005
JOURNAL FREE ACCESSEarly delayed gastric emptying has been reported as a frequent complication following pylorus preserving pancreatoduodenectomy (PPPD). We investigated the effect of division of the pyloric branch of the vagus nerve and/or transection of the duodenum on gastric emptying using the acetaminophen method in rats to speculate the unknown etiology of early delayed gastric emptying after PPPD. Twenty-four male Wistar rats were divided into the following four groups; Group S, sham operation as controls; Group N, disturbance of neuro-vascular supply to the pylorus; Group D, temporary interference of the duodenal continuity; and Group N+D, with both procedures in Group N and Group D. Gastric emptying was measured using the acetaminophen method at 1, 2, and 4 weeks after operations in each group. No significant difference was observed in Group S at any intervals after the operation. Gastric emptying was prolonged significantly in Group N, Group D and Group N+D compared to Group S until 2 weeks following surgery. Significant delayed gastric emptying was sustained in Group N+D at 4 weeks, although gastric emptying in Group N and Group D was improved by 4 weeks. The results in rodent models suggest that both dissection of the pyloric branch of the vagus and transection of the duodenum might be causative factors of postoperative delayed gastric emptying following PPPD.View full abstractDownload PDF (604K) -
Senji Hoshi, Ichiro Shintaku, Ken-ichi Suzuki, Toshiko Takahashi, Yasu ...2000 Volume 192 Issue 4 Pages 249-258
Published: 2000
Released on J-STAGE: March 29, 2005
JOURNAL FREE ACCESSTreatment by internal iliac arterial infusion chemotherapy (IA) combined with pelvic irradiation has proved to be effective for locally invasive bladder. Eight male patients, median age of 78 years (range 73-81) were enrolled. Pretreatment CT and whole layer core biopsy revealed T3a or T3b. Pelvic CT or fine needle aspiration biopsy following bipedal lymphography revealed N0 in 4 cases, N2 in 2 and N3 in 2, respectively. Three to 7 cycles of cisplatin (CDDP) 30-50 mg/m2, methotrexate 20 mg/m2 and tetrahydropymnyl-adriamycin 20 mg/m2 every 3 week was administered combined with 40-50 Gy. of whole pelvis irradiation. In 4 renal function impaired patients, 100 mg/m2 of carboplatin was administered instead of CDDP. All patients obtained complete response and the bladders were preserved. Observation periods were from 9 to 75 months (median 37 months). One N2 patient died with metastatic disease and two died without carcinoma. Two patients developed invasive bladder cancer on the side opposite to the primary tumors. Both were successfully treated by IA and irradiation. Bladders of all except one patient functioned for a long period. Side effects of IA and irradiation were not significant. IA combined with pelvic irradiation is effective and safe for elderly patients with bladder carcinoma.View full abstractDownload PDF (1536K) -
Kiminori Terui, Yoshihiro Takahashi, Junichi Kitazawa, Tsutomu Toki, M ...2000 Volume 192 Issue 4 Pages 259-273
Published: 2000
Released on J-STAGE: March 29, 2005
JOURNAL FREE ACCESSAlthough normal megakaryocytic development has been shown to require the presence of functional GATA-1 and NF-E2 transcription factors in vivo, the roles of other members of the GATA binding factors and NF-E2 family during megakaryocytic differentiation are unclear. In the present study, the expression of GATA family members, GATA-1 and GATA-2, a GATA-binding factor, EVI-1, the large subunit of NF-E2 factor, p45 and the related factors, Nrf1, Nrf2, Nrf3, BACH1, BACH2, and the small subunit of NF-E2, MAFK and MAFG has been examined in human megakaryocytic and erythroid cells by reverse transcriptase-polymerase chain reaction. CD34+ cells isolated from human cord blood were induced to unilineage megakaryocytic or erythroid differentiation in liquid suspension culture in the presense of thrombopoietin or erythropoietin, respectively. Each lineage was identified by monoclonal antibody against GPIIb/IIIa or glycophorin A. In megakaryocytic culture, p45, Nrf1, Nrf2, BACH1, MAFK and MAFG mRNAs were induced similarly to erythroid culture. Nrf3 mRNA was barely detected in both cultures. BACH2 was induced only in megakaryocytic culture, although the level of expression was low. Furthermore, the profiles of transcription factors involved in hematopoiesis, EVI-1 and Ets-1 mRNAs were induced only in megakaryocytic culture. Megakaryocytic and erythroid differentiation pathways are closely related to each other, and these two lineage cells share a number of lineage-specific transcription factors. However, the results showed that the profile of the expression of these transcription factors in megakaryocytic cells is distinct from that of erythroid lineage. The dynamic changes in the levels of different transcription factors that occur during primary megakaryocytic differentiation suggest that the levels of these factors may influence the progression to specific hematopoietic pathways.View full abstractDownload PDF (2315K) -
Shunji Suzuki, Yoshio Yoneyama, Rintaro Sawa, Tomoaki Murata, Tsutomu ...2000 Volume 192 Issue 4 Pages 275-281
Published: 2000
Released on J-STAGE: March 29, 2005
JOURNAL FREE ACCESSIn this study, we measured fetal plasma adenosine and xanthine concentrations during and after severe asphyxia, and investigated the key issues related to oxygen therapy. Asphyxia was induced by occluding the umbilical cord for 5 minutes in 6 fetal sheep with and without the administration of oxygen to the ewe. Plasma adenosine concentration increased significantly during cord occlusion in the all fetuses, and the differences between the values in the fetuses with and without maternal oxygen administration was not significant. By 30 minutes after cord release, plasma adenosine concentration in all fetuses had returned to levels similar to those at the start of the experiment. Plasma xanthine concentration also increased during cord occlusion in all fetuses. However, 30 minutes after cord release, plasma xanthine concentration had decreased significantly in fetuses without additional oxygen, while it did not change significantly in fetuses with maternal oxygen administration. Thus, we speculated that maternal oxygen administration before fetal asphyxia may not contribute to additional ATP stores in fetal organs and may produce oxygen free radicals following asphyxia.View full abstractDownload PDF (435K) -
Yutaka Masuda, Minoru Suzuki2000 Volume 192 Issue 4 Pages 283-289
Published: 2000
Released on J-STAGE: March 29, 2005
JOURNAL FREE ACCESSForced swimming is considered a depression model. Repeated electroconvulsive shock treatment shows an anti-depressive effect in mice forced swimming. In serum of the mice treated with repeated electroconvulsive shock, the humoral anti-depressive factors were detected. The factors were the glycolipid having GalNAcα1-3GalNAc and mouse fibrinopeptide A having amino acid sequence TDTEKDGEFLSGGGV. The behavioral anti-depressive activity of the glycolipid was decreased by the low doses (100∼10 μg/kg) of dopamine 2 receptor antagonist sulpiride. Behavioral activity of the peptide was also decreased by the low doses (100∼1 μg/kg) of dopamine 1 receptor antagonist SCH-23390. The present findings clearly indicate that repeated electroconvulsive shock treatment induces the humoral anti-depressive factors affecting the dopaminergic neuronal system in mice.View full abstractDownload PDF (407K) -
Yoshiko Tobimatsu, Ryuichi Nakamura2000 Volume 192 Issue 4 Pages 291-299
Published: 2000
Released on J-STAGE: March 29, 2005
JOURNAL FREE ACCESSThe purpose of this study was to investigate the characteristics of individuals with cerebral palsy that affected their ability to find a job in Japan. A retrospective nonrandomized descriptive study was performed. Subjects were 99 individuals with cerebral palsy who were eligible to have a vocational training at the National Rehabilitation Center for the Disabled after graduation from high school. All of them were able to perform ADL unassistedly. The mean age of the subjects was 19.9 years (range, 18 to 33) and the mean intelligence quotient measured by WAIS-R was 78.5 (range, 46 to 110). Walking ability, being female and experience of learning in a regular middle high school were significant explanatory variables in the multiple regression equation. The ability of individuals with cerebral palsy to get a job in Japan in the 1990's was largely determined by being able to walk and having an education in a regular school.View full abstractDownload PDF (523K) -
Mami Akazawa, Masaru Takasaki, Akio Tomoda2000 Volume 192 Issue 4 Pages 301-312
Published: 2000
Released on J-STAGE: March 29, 2005
JOURNAL FREE ACCESSWhen human erythrocytes were incubated with ο-aminophenol at pH 7.0 at 37°C for 46 hours, intracellular oxyhemoglobin was completely oxidized to methemoglobin during the initial 6 hours, and methemoglobin formed was then reduced to oxyhemoglobin during the following 20 hours. This was demonstrated by the changes in absorption spectra of intracellular hemoglobin. Such oscillatory behavior of intracellular hemoglobin during reaction with ο-aminophenol was explained by the fact that ο-aminophenol has the ability to both oxidize oxyhemoglobin and reduce methemoglobin. In order to study the mechanism of oxido-reductive reactions of hemoglobin with aromatic reductants including ο-aminophenol, the oxidation of ferrous hemoglobin and reduction of methemoglobin with various aromatic reductants such as ο-aminophenol, 2-amino-4-methyl-phenol, 2-amino-5-methylphenol, and homogentisic acid were investigated under various conditions. It was found that oxyhemoglobin was oxidized by these aromatic compounds, and the oxidation rate was accelerated in the presence of inositol hexaphosphate, but was not affected in the presence of catalase and superoxide dismutase, except for the case with homogentisic acid. The oxidation of ferrous hemoglobin by these compounds did not proceed under anaerobic conditions. Methemoglobin was reduced by these aromatic compounds, and the reduction rate was much accelerated in the presence of inositol hexaphosphate, but was not affected in the presence of catalase and superoxide dismutase, except for the case with homogentisic acid. The reduction of methemoglobin by these compounds proceeded under anaerobic conditions, suggesting that ferric heme of hemoglobin reacts directly with aromatic reductants. On the basis of these results, the mechanism of oxido-reductive reaction of ferrous and ferric hemoglobin with aromatic reductants was proposed.View full abstractDownload PDF (659K)
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