SEINO, J., FUKUOKA, Y., OKUDA, T. and TACHIBANA, T.
Isolation, Molecular Properties and Allotype of Mouse Clq. Tohoku J. exp. Med. 1984,
142 (4), 351-361-Mouse Clq, a subcomponent of the first component of complement, was purified from mouse EDTA plasma by a combination of precipitation with polyethyleneglycol, affinity chromatography on IgG-Sepharose, ion exchange chromatography and molecular sieving. Mouse Clq was compatible with human Clq in the sense that it shows Cl hemolytic activity by the combination with human Clr and Cls. The molecular weight of mouse Clq as estimated by SDS-PAGE was approximately 410, 000 and almost the same as that of human Clq. After treatment of mouse Clq with 4M urea, three distinct subunits were obtained on SDS-PAGE in non-reducing condition. Moreover it was shown that there are small, yet definite differences in the molecular weights of the subunits between the two mouse strains. Three subunits of the Clq from C3H and ICR showed apparent molecular weights of 62, 000, 58, 000 and 53, 000 but those of DDI showed 62, 000, 55, 000 and 51, 000. These results suggested the existence of allotype of mouse Clq, and indeed, alloantisera made by injecting the Clq of ICR to DDI reacted in Ouchterlony double diffusion test with the plasma of ICR, C3H, AKR and BALB/c, but not with DDD, DKI, C57BL/10 and C57BL/6. -mouse Clq; subunits of mouse Clq; allotype of mouse Clq
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