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Volume 145 (1985)
- Issue 4 Pages 353-
- Issue 3 Pages 233-
- Issue 2 Pages 105-
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Volume 145, Issue 3
Displaying 1-13 of 13 articles from this issue
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Determination of the Normal Range for the Diagnosis of Autonomic NeuropathyNOBORU OIKAWA, MASANORI UMETSU, TAKAYOSHI TOYOTA, YOSHIO GOTO1985 Volume 145 Issue 3 Pages 233-241
Published: 1985
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSOIKAWA, N., UMETSU, M., TOYOTA, T. and GOTO, Y. Quantitative Evaluation of Diabetic Autonomic Neuropathy by Using Heart Rate Variations - Determination of the Normal Range for the Diagnosis of Autonomic Neuropathy. Tohoku J. exp. Med., 1985, 145 (3), 233-241 - Heart rate (HR) variations -in supine resting position, during deep breathing and on standing - were measured in 162 healthy subjects and 168 diabetics by use of an instantaneous-HR-change continuous recorder. As indices of HR variations, the standard deviation of the HR at rest (SD of HR), the mean difference between maximal and minimal HR during deep breathing (ΔI-E) and the HR increase on standing (ΔHR) were determined. In healthy subjects, the values for each test declined with age and the log-transformed data fitted the linear regression. The 90% confidence limits were calculated for the normal range and the values below normal range were defined as abnormal. In diabetics, the incidence of abnormal response were 19% in the SD of HR, 38% in the ΔI-E and 22% in the ΔHR. The ΔI-E was the most sensitive index for the autonomic neuropathy. The ΔHR was considered to be able to detect the different mechanisms of neural reflexes because of the poor correlation between the ΔHR and the respiratory HR variations. The present studies suggested that ΔI-E and ΔHR should be measured at the same time to evaluate the autonomic neuropathy.View full abstractDownload PDF (740K) -
YASUE FUKUSHI, REIKO YAMAMOTO1985 Volume 145 Issue 3 Pages 243-249
Published: 1985
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSFUKUSHI, Y. and YAMAMOTO, R. Effects of Methylmercuric Chloride and Bis (Methylmercuric) Selenide on Responses of Isolated Intestinal Smooth Muscle of the Guinea-Pig. Tohoku J. exp. Med., 1985, 145(3), 243-249 - The neurologically toxic substance, methylmercury, is detoxicated by simultaneous application of selenite. In vivo formation of bis (methylmercuric) selenide has been suggested as the mechanism of detoxication by selenite. By using isolated guinea-pig taenia coli preparations, the effects of methylmercuric chloride and bis (methylmercuric) selenide on contractile responses to nerve and direct stimulation were investigated. Application of either drugs scarcely affected contractile responses to direct stimulation. However, contractile responses to nerve stimulation were severely reduced by methylmercuric chloride. The effect of bis (methylmercuric) selenide was considerably weaker than methylmercuric chloride. The site blocked by methylmercuric chloride and bis (methylmercuric) selenide in the taenia preparation was discussedView full abstractDownload PDF (649K) -
HIROSHI MUNAKATA, MAMORU ISEMURA, ZENSAKU YOSIZAWA1985 Volume 145 Issue 3 Pages 251-257
Published: 1985
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSMUNAKATA, H., ISEMURA, M. and YOSIZAWA, Z. An Application of the High-Iron Diamine Staining for Detection of Sulfated Glycoproteins (Glycopeptides) in Electrophoresis on Cellulose Acetate Membrane. Tohoku J. exp. Med., 1985, 145 (3), 251-257-The high-iron diamine staining (HID), which has been used in histochemistry to stain sulfated glycoconjugates (SGC), was tested for delectability of authentic acidic substances (chondroitin sulfates A plus C, dermatan sulfate, heparan sulfate, chondroitin, hyaluronic acid, α1-acid glycoprotein and ribonucleic acid) in electrophoresis on cellulose acetate membrane (Separax). The results showed that only SGC were detectable by the HID, although all the above substances were stained with alcian blue. The glycoconjugate preparations obtained from the liver, kidney, lung, small intestine, colon, stomach, brain and spleen of rats were examined by two-dimensional electrophoresis on Separax. The new spots (or bands), besides those of sulfated glycosaminoglycans, were detected by the HID on the electrophoretograms of all the samples except for the kidney one. The substances giving the new spots (or bands) were indicated to be sulfated glycopeptides (SGP) by crude heparinase digestion of a representative sample. The present results revealed that the HID was applicable for detection of SGP in electrophoresis on cellulose acetate membrane. Also, it is a novel finding that the liver and spleen contain SGP.View full abstractDownload PDF (3577K) -
TOYOHARU ISAWA, TAKEO TESHIMA, TOMIO HIRANO, AKIO EBINA, KIYOSHI KONNO1985 Volume 145 Issue 3 Pages 259-267
Published: 1985
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSISAWA, T., TESHIMA, T., HIRANO, T., EBINA, A. and KONNO K. Estimation of Alveolar Deposition Ratio of Inhaled Radioaerosol. Tohoku J. exp. Med., 1985, 145 (3), 259-267 - The lung retention ratio (LRR) and the alveolar deposition ratio (ALDR) are mandatory to calculate the other three indices for quantitatively evaluating mucociliary clearance function of the ciliated airways; the airway deposition ratio (ADR), the airway retention ratio (ARR) and the airway clearance efficiency (ACE). According to our original method, the LRR required 2-hr continuous measurement of radioactivity in the thorax and the ALDR a repeat measurement at 24-hr after radioaerosol inhalation. The 2-hr continuous measurement is, however, too long for a clinical examination and the 24-hr repeat measurement cumbersome. The purpose of the study was to find a way to get the ALDR by calculation without repeating the 24-hr measurement and by counting radioactivity for the shortest possible period and yet without sacrificing the visual evaluation by compiling radioaerosol inhalation lung cine-scintigraphy. By using the derived formulae listed in Table 1, the ALDR was calculable. Forced expiratory volume (FEV) in one sec divided by FEV in per cent (FE V1.0%), the initial 60min measurement of radioactivity without repeating 24-hr measurement, and smoking history were the minimum requirements to calculate the ALDR. The calculated ALDR appeared reliable enough to estimate the other indices.View full abstractDownload PDF (767K) -
RYUJI NAKAMURA1985 Volume 145 Issue 3 Pages 269-282
Published: 1985
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSNAKAMURA, R., Quantitative Light and Electron Microscopical Studies of the Effect of Vagotomy on Parietal Cells in Rats. Tohoku J. exp. Med., 1985, 145 (3), 269-282 - As early as 3 days after truncal vagotomy, a decrease in the number of parietal cells per gland and the length of fundic glands of the stomach was observed in young Wistar rats. A decrease in the size of cells and the number of mitochondria per cell was also evident in the remaining parietal cells. These atrophic changes lasted nearly one month after the operation. A decrease in the cellular size and mitochondrial number in parietal cells was noted again in the rats sacrificed 12 months after the operation. Since the similar changes were also seen in the age-matched controls, the changes in older rats were interpreted as an aging phenomenon.View full abstractDownload PDF (3093K) -
MINEO IWASA, ISAO OHYA, TSUYOSHI YOKOI, KAORU SAGISAKA1985 Volume 145 Issue 3 Pages 283-290
Published: 1985
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSIWASA, M., OHYA, I., YOKOI, T. and SAGISAKA, K. A New Individual Variation of Saliva Detected with Rabbit Anti-Human Saliva Serum. Tohoku J. exp. Med., 1985, 145(3), 283-290 - A rabbit anti-saliva hyperimmune serum was raised against pooled human saliva. After absorption with human serum and semen, the immune serum formed two or three distinct precipitation lines with human saliva by the Ouchterlony method. Based on the presence or absence of a precipitation line, close to the antigen well designated as Pl, all human saliva were divided into two groups; Pl+ and Pl-. The incidence of Pl+ saliva was 114 out of 154 samples (74%). By immunoelectrophoresis with anti-saliva serum preabsorbed with Pl- saliva, Pl+ saliva formed a precipitation line in cathodal area. No amylase activity was found in this precipitation line but it was stained with the Schiff's reagent. Pl protein was isolated by Sephadex G-100 and ion-exchange chromatography. Isoelectric focusing of the Pl protein in polyacrylamide salb gel containing urea revealed that the protein differed from any known parotid salivary proteins such as Pr, Db, Pa and Pm.View full abstractDownload PDF (1904K) -
SEIKI MATSUNO, MASAO KOBARI, HIROTAKE HISANO, HIDEMI YAMAUCHI, TOSHIO ...1985 Volume 145 Issue 3 Pages 291-302
Published: 1985
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSMATSUNO, S., KOBARI, M., HISANO, H., YAMAUCHI, H. and SATO, T. Prognosis of the Pancreatic Cancer in Terms of the Regional Lymph Node Reaction. Tohoku J. exp. Med., 1985, 145 (3), 291-302 - In order to elucidate the immunological defense mechanism in tumor bearing host against pancreatic cancer, tissue reactions of the regional lymph nodes were investigated in terms of extension and prognosis of pancreatic cancer. As the site for investigation of lymph node, paracortical area, germinal center and sinus histiocytes system were selected. In each case paracortical hyperplasia, of hyperplasia germinal center and sinus histiocytosis were graded and the relationship between such grade and prognosis of resected pancreatic cancer was studied with the following results. The grades of each tissue reaction in cases without lymph node metastasis were higher than that observed in cases with metastasis. There was significant difference in sinus histiocytosis (p<0.01). Higher grade tissue reactions were observed in cases with primary lesion with histologic type classified as papillary adenocarcinoma or as well differentiated tubular adenocarcinoma as compared to those in cases of poorly differentiated histologic type. In cases with pancreatic cancer the prognosis was better than cases with higher grade of tissue reactions of the regional lymph nodes. In cases with pancreatic cancer both cellular immunity and humoral immunity are involved in host defense mechanism. It was considered that sinus histiocytosis is significant as an indicator for good prognosis and a factor to inhibit progression in pancreatic cancer.View full abstractDownload PDF (2508K) -
TAKAYOSHI TODA, YUMIKO TODA, FRED A. KUMMEROW1985 Volume 145 Issue 3 Pages 303-310
Published: 1985
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSTODA, T., TODA, Y. and KUMMEROW, F.A. Coronary Arterial Lesions in Piglets from Sows Fed Moderate Excesses of Vitamin D. Tohoku J. exp. Med., 1985, 145(3), 303-310 - Ultrastructural studies were conducted on the coronary arteries of 6 week-old piglets from sows which received diets containing either 25 μg or 3.7μg of vitamin D3 per pound of basal ration. The subendothelial space which was rich in mucopolysaccharide and newly formed tiny elastic fibers, contained numerous macrophages, some of which possessed lipid droplets. Degenerated smooth muscle cells with pyknotic nuclei or vesiculated organelles, and mitotic smooth muscle cells were occasionally seen in the coronary artery. Piglets from sows which were fed high doses of vitamin D had more degenerated smooth muscle cells without stainable lipid and lipid containing cells in their coronary arteries than piglets from sows which were fed low doses of vitamin D. These results suggested that an excess dietary intake of vitamin D by pregnant animals may have potential angiotoxic effects on the coronary arteries of their offsprings.View full abstractDownload PDF (3970K) -
YOSHIO UEHARA, SHINSEI GASA, AKIRA MAKITA, KEISUKE SAKURADA, TAMOTSU M ...1985 Volume 145 Issue 3 Pages 311-319
Published: 1985
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSUEHARA, Y., GASA, S., MAKITA, A., SAKURADA, K. and MIYAZAKI, T. Expression of Arylsulfatase B Variant from Leukocytes in Chronic Myelogenous Leukemia Related to Chemotherapy. Tohoku J. exp. Med., 1985, 145(3), 311-319 -Lysosomal arylsulfatase B of human leukocytes consisted of two forms; a basic form (B) and a variant form (B1) which is phosphorylated at the carbohydrate chains of the B form (Uehara, Y., Gasa, S., Makita, A., Sakurada, K., and Miyazaki, T. (1983) Cancer Res. 43, 5618-5622). The amounts of the variant form relative to the basic form were considerably increased in leukocytes of chronic myelogenous leukemia (CML). The present communication demonstrates that, upon chemotherapy of the patients with CML, degree of phosphorylation as well as the relative amounts of the phosphorylated variant form of CML leukocytes are markedly decreased concomitantly with an increase of the basic, less phosphorylated form. This effect of chemotherapy on the variant form preceded to clinical improvement of the CML patients, suggesting that the relative amount of the phosphorylated enzyme will be a potential prognostic indicator for the therapeutic effect of CML.View full abstractDownload PDF (862K) -
TETSUO SHIMIZU, YUKO ISHIKAWA, ATSUKO FURUTA, TSUNEO FUKUDA1985 Volume 145 Issue 3 Pages 321-328
Published: 1985
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSHIMIZU, T., ISHIKAWA, Y., FURUTA, A. and FUKUDA, T. Biochemical and Membrane Functional Alterations in Red Cells during Preparation and Storage of Leukocyte- and Platelet-Poor Red Cell Suspensions Prepared by Warm-Centrifuge Method. Tohoku J. exp. Med., 1985, 145 (3), 321-328 - A simple technique for effective removal of leukocytes and platelets from red blood cells (RBC) was reported. In that technique whole blood was incubated at 3°C. In its modified procedure phosphate buffer was added to the starting RBC. Changes in red cells during preparation and storage at 4°C of RBC suspensions were evaluated. Reconstituted RBC were incubated at 37°C for 1hr. After centrifugation, the lower compartment was separated with a special apparatus and diluted with 0.9% saline, which contained 78% red cells and 3% leukocytes and platelets of the starting RBC. In the incubation and subsequent preparation steps, 2, 3-diphosphoglycerate levels were decreased, but not adenosine triphosphate. One-day storage caused no serious metabolic changes in red cells. Red cell osmotic fragility was not changed throughout. RBC stored maintained sterility. The in vitro data indicate that red cells in leukocyte- and platelet-poor RBC suspensions processed with the warm-centrifuge method retained high biochemical and membrane functional abilities for 1 day.View full abstractDownload PDF (658K) -
YASUO OSHIMA1985 Volume 145 Issue 3 Pages 329-333
Published: 1985
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSOSHIMA, Y. Effect of Diet Restriction on 24-hr Urinary C-Peptide Excretion in Non-Insulin Dependent Non-Obese Diabetic Subjects. Tohoku J. exp. Med., 1985, 145 (3), 329-333 - Changes in insulin secretory responses by diet restriction were analyzed in non-insulin dependent non-obese diabetic subjects (NIDDM -NO) as well as in healthy volunteers by determining 24-hr urinary C-peptide immunoreactivity (24-hr UCPR). Insulin sensitivity for glucose utilization was measured by using cyclic somatostatin. Overnight fasting plasma glucose concentrations decreased in NIDDM-NO; however, 24-hr UCPR was reduced in both NIDDM-NO and healthy controls by diet restriction (25kcal/kg) for 14 days. The level of 24-hr UCPR was higher but insulin sensitivity was lower in NIDDM-NO as compared with those in healthy controls. Therefore, diet restriction may be effective in controlling hyperglycemia and hypersecretion of insulin in NIDDM-NO, but it alone may not improve the reduced insulin sensitivity.View full abstractDownload PDF (416K) -
RYUICHI NAKAMURA, TOORU HOSOKAWA, ICHIRO TSUJI1985 Volume 145 Issue 3 Pages 335-340
Published: 1985
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSNAKAMURA, R., HOSOKAWA, T. and TSUJI, I. Relationship of Muscle Strength for Knee Extension to Walking Capacity in Patients with Spastic Hemiparesis. Tohoku J. exp. Med., 1985, 145 (3), 335-340 - Muscle strength for knee extension, both isokinetic and isometric, and walking capacity, maximum velocity and walking rate, were examined in 11 post-stroke hemiparetic patients. The degree of spasticity of the affected lower extremity was not related to the isokinetic and isometric torques, or to the walking capacity. Although both the isokinetic and isometric torques decreased on the affected side compared to the non-affected side, the rate of decrease was remarkable in isokinetic torque at fast velocities. The walking capacity was well correlated with isokinetic torque of the affected side at fast velocities but not isometric torques. These results suggest that reduction of muscle strength at rapid movements is an essential feature of spastic paresis and is primary cause of motor disabilities such as impaired locomotor function.View full abstractDownload PDF (579K) -
TAKEHIRO MITSUHASHI, YORIHIKO CHANOKI, HIDEKI KUWAHARA, TAKEO SAKAI, H ...1985 Volume 145 Issue 3 Pages 341-351
Published: 1985
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSMITSUHASHI, T., CHANOKI, Y., KUWAHARA, H., SAKAI, T., MASUDA, H. and SUGINO, S. Experimental Emphysematous Bullae Induced by Methylchoranthrene and Carrageenan. Tohoku J. exp. Med., 1985, 145 (3), 341-351 - An animal model for the emphysematous bullae in the rabbit lung induced by methylchoranthrene and carrageenan was reported. In early stages (by 2 months) the cystic lesions associated with extensive pneumonia. In later stages the inflammation subsided and only the cystic lesions remained, resulting in typical, large emphysematous bullae.View full abstractDownload PDF (3693K)
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