The Tohoku Journal of Experimental Medicine
Online ISSN : 1349-3329
Print ISSN : 0040-8727
ISSN-L : 0040-8727
Volume 173 , Issue 4
Showing 1-8 articles out of 8 articles from the selected issue
  • YUKIE SAITOR, MASAHITO MIURA, HIROFUMI NIITSUMA, NOBORU NUMATA, HITOSH ...
    1994 Volume 173 Issue 4 Pages 361-369
    Published: 1994
    Released: August 31, 2006
    JOURNALS FREE ACCESS
    SAITOH, Y., MIURA, M., NIITSUMA, H., NUMATA, N., OHORI, H., ISHII, M. and TOYOTA, T. Changes of Serum Hepatitis C Virus Levels in Patients with Chronic Hepatitis C Treated with Two Courses of Interferon Administration. Tohoku J. Exp. Med., 1994, 173 (4), 361-369-To assess the efficacy of repeated interferon (IFN) administration in patients with chronic hepatitis C unresponsive to initial therapy, serum hepatitis C virus (HCV)-RNA levels were measured in 12 patients who had failed prior IFN therapy. Serum HCV-RNA was assayed by measuring DNA complementary to HCV-RNA using a quantitative reverse transcription-polymerase chain reaction assay. The mean total dose of IFN was 227.8 megaunits for first treatment and 270.7 mega-units for second treatment. Five responders with a normal alanine aminotransferase (ALT) concentration (less than 40IU/liter) at the end of the first treatment also had a normal ALT concentration at the end of the second treatment. By contrast, all nonresponders with an elevated ALT concentration during the first treatment likewise had an elevated ALT concentration at the end of the second treatment. HCV-RNA levels before the first treatment varied from 106 to 108 copies/μl. The serum HCV-RNA levels fell in 9 out of 10 patients after the first treatment and in 11 out of 12 patients after the second treatment. One patient had unchanged normal serum ALT levels after two courses of IFN treatment. These results suggested that the outcome of a second course of IFN treatment was similar biochemically and virologically to a first course, and that patients who did not respond initially seldom respond to additional IFN therapy. Therefore, readministration of IFN should be restricted to patients who respond biochemically and virologically to initial treatment. The optimal second dose shall be determined with further studies.
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  • HIDEKI YUKI, SHINOBU SATO, YASUSHI ARISAKA, SHUICHI KATO, HITONOBU TOM ...
    1994 Volume 173 Issue 4 Pages 371-375
    Published: 1994
    Released: August 31, 2006
    JOURNALS FREE ACCESS
    YUKI, H., SATO, S., ARISAKA, Y., KATO, S. and TOMOIKE, H. Orally Administered Beraprost Sodium Inhibits Pulmonary Hypertension Induced by Monocrotaline in Rats. Tohoku J. Exp. Med., 1994, 173 (4), 371-375-Rats were fed on distilled water (DW) or DW containing beraprost sodium (BPS). BPS concentration was 1.5 or 3.0μ/ml in DW for the low BPS groups and 6.0 or 10.0μ/ml in DW for the high BPS group. Monocrotaline (MCT) was subcutaneously given (60 mg/kg), and saline was injected as control. Data were analyzed among the following groups; Groups (Saline+DW), (Saline+Low BPS), (MCT+DW), (MCT+Low BPS) and (MCT+High BPS). Three weeks later, pulmonary (Ppa) and systemic (Psa) arterial pressure were measured under anesthesia. MCT caused significant elevation of Ppa {18.3±0.6cmH2O for Group (Saline+DW) vs. 27.2± 1.2cmH2O for Group (MCT+DW), p<0.001, mean±S.E.} and Ppa was significantly and dose-dependently suppressed by BPS; Group (MCT+DW) vs. Groups (MCT+Low BPS), 23.4±0.7cmH2O and (MCT+High BPS), 22.5±0.5 cmH2O, p<0.05, mean±S.E.). Psa was not lowered dose-dependently by BPS. We conclude that oral beraprost sodium suppresses pulmonary hypertension produced by monocrotaline in rat.
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  • AKIHIRO MUNAKATA, YOSHIHARU UNO
    1994 Volume 173 Issue 4 Pages 377-382
    Published: 1994
    Released: August 31, 2006
    JOURNALS FREE ACCESS
    MUNAKATA, A. and UNO, Y. Colonoscopic Polypectomy with Local Injection of Methylene Blue. Tohoku J. Exp. Med., 1994, 173 (4), 377-382-For safer and complete colonoscopic polypectomy of large broad-based adenomas, we attempted submucosal injection of methylene blue before electrosurgical snare excision. Twenty-five large broad-based sessile adenomas 20mm or more in diameter were resected. Methylene blue was injected about 2mm away from the edge of the lesion. After injection, the lesion appearing as a blue bleb was resected by snare polypectomy. All the lesions were completely resected by single-step polypectomy. During and after polypectomy, no complication occurred. Endoscopic resection of large sessile adenomas with the aid of submucosal injection of methylene blue thus appears to be safe and effective.
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  • KOUHEI FUKUSHIMA, HIRONOBU SASANO, IWAO SASAKI, HIROSHI NAGURA, YUJI F ...
    1994 Volume 173 Issue 4 Pages 383-390
    Published: 1994
    Released: August 31, 2006
    JOURNALS FREE ACCESS
    FUKUSHIMA, K., SASANO, H., SASAKI, I., NAGURA, H., FUNAYAMA, Y., MATSUNO, S. Increased Expression of Mineralocorticoid Receptor in Human Ileum after Total Colectomy: Immunohistochemical and Immunoblotting Studies. Tohoku J. Exp. Med., 1994; 173 (4), 383-390-Aldosterone is known to regulate transmembrane ion transport and water absorption through its binding to mineralocorticoid receptor (MR) in the mammalian colon. A possible roles of aldosterone has been suggested in increased water absorption from remnant ileum in the patients with total colectomy. We, therefore, studied immunolocalization of MR in remnant ileal mucosa in the patients with total colectomy in order to study the role of aldosterone in water and sodium absorption in these patients. Immunohistochemical localization of MR was performed in ileal mucosa of 7 patients with total colectomy and 5 cases of normal ileum obtained from the resection of ascending colon due to carcinoma by using a polyclonal antibody raised against a mineralocorticoid receptor fusion protein as a primary antibody and a biotin-streptavidin system for immunostaining. Immunoblotting was also performed. Positive MR immunoreactivity was observed in both cytoplasm and nucleus of absorptive cells of ileum of total colectomy patients but not in control normal ileum. Immunoblotting revealed the presence of an approximately 100kDa immunoreactive product corresponding to mineralocorticoid receptor. Aldosterone is considered to act on ileal mucosa following total colectomy and the aldosterone dependent sodium transport and water absorption may be accelerated in ileal mucosa after total colectomy, which is consistent with the postoperative decrease in the stool volume observed in these patients. In conclusion, increased expression of the mineralocorticoid receptor in remnant ileum may play an important role in intestinal adaptation in total colectomized patients.
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  • HIROYUKI KIKUCHI, TOKUTARO SATO
    1994 Volume 173 Issue 4 Pages 391-397
    Published: 1994
    Released: August 31, 2006
    JOURNALS FREE ACCESS
    KIKUCHI, H. and SATO, T. Colorimetric Microdetermination of l-Lactate in Deproteinized Blood. Tohoku J. Exp. Med., 1994, 173 (4), 391-397-The method by MacQueen and Plaut was modified to devise simple microdetermination method of l-lactate which is applicable to deproteinized blood sample. Blood was deproteinized with addition of 25% (w/w) trichloroacetic acid (TCA), and 0.075 M 2-amino-2-methyl-1, 3-propanediole (AMPD) was used to make pH of the reaction mixture optimal. It was validated in recovery and precision study, and showed good correlation with usual UV method; correlation coefficient was 0.985, and slope was 0.994. TCA-blood mixture may be kept at room temperature for at least 2hr without centrifugation, and the supernatant may be preserved for at least 2 weeks at 4°C.
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  • TOKUTARO SATO, MASAO HIWATARI
    1994 Volume 173 Issue 4 Pages 399-404
    Published: 1994
    Released: August 31, 2006
    JOURNALS FREE ACCESS
    SATO, T. and HIWATARI, M. Control of Diabetes during Rehabilitation for Diabetic Stroke. Tohoku J. Exp. Med., 1994, 173 (4), 399-404-Changes of diabetic control during rehabilitation therapy was studied retrospectively in 33 cases of diabetic stroke. Diabetic control was not deteriorated in any cases during rehabilitation therapy. In 21 cases, treatment of diabetes was not changed during rehabilitation therapy and the levels of fasting plasma glucose were decreased in all of them. Doses of hypoglycemic agents was reduced without significant deterioration of diabetic control in 9 cases whose diabetic control was excellent, and in 2 cases who experienced hypoglycemic attack during rehabilitation therapy. In these cases, oral hypoglycemic agent was reduced from 2 to 1 tablet and insulin from 32 to 21 units per day on average. These results indicate that training in the stroke rehabilitation moderately improves diabetic control.
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  • SHIGERU KOHNO, HIRONOBU KOLA, MIKIO OKA, JYUN-ICHI KADOTA, MITSUO KAKU ...
    1994 Volume 173 Issue 4 Pages 405-411
    Published: 1994
    Released: August 31, 2006
    JOURNALS FREE ACCESS
    KOHNO, S., KOGA, H., OKA, M., KADOTA, J., KAKU, M., SODA, H., TOMONO, K. and HARA, K. The Pattern of Respiratory Infection in Patients with Lung Cancer. Tohoku J. Exp. Med., 1994, 173 (4)-405-411-We examined retrospectively the pattern of respiratory infection in 579 patients with lung cancer admitted to Nagasaki University Hospital during the past 15 years. A total of 139 patients (24.0%) developed respiratory infection. The rates of pulmonary infection associated with large (36.2%) and small cell carcinomas (33.6%) were significantly higher than those with squamous cell carcinoma (26.0%) and adenocarcinoma (17.3%). Advanced stages of lung cancer were associated with higher complication rates (stage I: 6.3%, stage II: 15.9%, stage III: 27.9%, and stage IV: 33.8%). Deceased patients showed a significantly higher rate of pulmonary infection than alive patients during the period of investigation. Isolated organisms in excess of 107cfu/ml in sputum or 104cfu/ml in bronchial aspirate were mainly gram-negative bacteria (68.8%), such as Haemophilus influenzae, Klebsiella pneumoniae, Enterobacter cloacae, Acinetobacter sp. and Pseudomonas aeruginosa. The number of patients infected with gram-positive bacteria increased markedly after 1982. Our results suggest that a successful control of pulmonary infection associated with lung cancer is important in improving the prognosis of lung malignancy.
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  • TERUO NAKAMURA, KAZUO TAKEBE, KENJI KUDOH, MASATAKA ISHII, KEN-ICHI IM ...
    1994 Volume 173 Issue 4 Pages 413-420
    Published: 1994
    Released: August 31, 2006
    JOURNALS FREE ACCESS
    NAKAMURA, T., TAKEBE, K., KUDOH, K., ISHII, M., IMAMURA, K., KIKUCHI, H., KASAI, F., TANDOH, Y., YAMADA, N., ARAI, Y., TERADA, A. and MACHIDA, K. Increased Plasma Gluconeogenic and System A Amino Acids in Patients with Pancreatic Diabetes due to Chronic Pancreatitis in Comparison with Primary Diabetes. Tohoku J. Exp. Med., 1994, 173 (4), 413-420-We examined the plasma amino acid profiles of 17 patients with pancreatic diabetes in comparison with those of 14 healthy subjects and 16 patients with primary diabetes of similarly poor glycemic control. We also measured fasting plasma glucagon and free insulin levels in patients with pancreatic diabetes and in those with primary diabetes. The fasting plasma amino acid level was highest in patients with pancreatic diabetes. Furthermore, the plasma glucagon level in patients with pancreatic diabetes was significantly low, and negative correlations were found between glucagon and glucagon-related System A and gluconeogenic amino acids. On the other hand, the levels of branched-chain amino acids (BCAA) and System L amino acids, which are subjected to change in the state of diabetes mellitus, increased in patients with pancreatic diabetes. From the above results, we determined that the amino acid profile in patients with pancreatic diabetes resembled that in patients with total pancreatectomy. It is our opinion that the increases of amino acid levels (gluconeogenic and System A amino acids) in patients with pancreatic diabetes is not dependent on maldigestion/malabsorption under treatment, but is mainly dependent on glucagon deficiency.
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