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Volume 89 (1966)
- Issue 4 Pages 307-
- Issue 3 Pages 213-
- Issue 2 Pages 103-
- Issue 1 Pages 1-
Volume 89, Issue 3
Displaying 1-8 of 8 articles from this issue
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Shigeo Kariyone, Takeo Miyake1966 Volume 89 Issue 3 Pages 213-234
Published: July 25, 1966
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSQualitative and quantitative observations of stainable iron in the bone marrow reticulum cell and in the sideroblast were performed in various hematological disorders of human subjects.
Histological and metabolic studies of iron in reticulum cells of the liver, spleen and bone marrow after the administration of colloidal iron and Mohr's salt were carried out in rabbits under various experimental conditions.
1) Hemosiderin granules in the bone marrow reticulum cell varied in various hematological disorders, not only in their amount, but also in their form and nature.
2) There was a moderate correlation between the number of iron granules in the bone marrow reticulum cell and in sideroblasts. Both of them were well correlated to the ability of erythropoiesis.
3) In animal experiments, stainable iron in the reticulum cell of the liver, spleen and bone marrow were markedly increased 4 hours after the injection of colloidal iron. After that, iron in the cell was re-distributed to other tissues according to the various experimental conditions. When a large dose of Mohr's salt was injected, iron was taken chiefly by the parenchymal cell of the liver, and then it was eluted to plasma and saturated transferrin for several hours.
Kinetics of hemosiderin iron in the reticulum cell was studied under various experimental conditions.
4) From the study of the hemosiderin turnover in reticulum cells, it was suggested that there might be a functional difference between the reticulum cell of the spleen and of the bone marrow.
5) The frequency of phagocytosis of erythroblasts and erythrocytes in the bone marrow reticulum cell was studied in clinical cases and in experimental animals, and its significance was discussed.View full abstractDownload PDF (2775K) -
Akira Yajima1966 Volume 89 Issue 3 Pages 235-244
Published: July 25, 1966
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe cerebrum, cerebellum, diencephalon and brain stem of female rats were studied for their contents of nucleic acid bases, and the values obtained were compared between maturity and decrepitude.
There was recognized no difference in contents of the DNA bases, i.e., guanine (G), adenine (A), cytosine (C) and thymine (T), among the portions of the brain as well as between maturity and decrepitude. Contents of RNA bases guanine, adenine, cytosine and uracil (U) did not show any variation according to portions of the brain both in mature and decrepit rats; however, in the latter, as compared with the former, the four portions of the brain all gave a lower value of guanine and a higher level of uracil; thus there was noted a declining tendency of (G+C) /(A+U).
In comparison of the nucleic acid fractions of the diencephalon between mature and decrepit rats, the RNA/DNA ratio was reduced in the latter as compared with that in the former. s-RNA/DNA, r1-RNA/DNA and r2-RNA/DNA were found lower in decrepit than those in mature rats. Especially r2-RNA/DNA, that is the ratio of the high molecular portion of ribosome RNA to DNA, was markedly reduced.View full abstractDownload PDF (590K) -
Seigi Tsuchida, Yukio Kimura1966 Volume 89 Issue 3 Pages 245-250
Published: July 25, 1966
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe post-surgical effects of ureterostomy and nephrostomy were compared, and the following results were obtained: (1) reduction of renal function was greater after ureterostomy than after nephrostomy and (2) pyelonephritis occurred more frequently following ureterostomy than nephrostomy. In addition, electoureterography and urometrography were conducted in 10 cases following ureterostomy. One case of them showed normal ureteral peristalsis, 4 cases showed irregular peristalsis, and in the remaining 5 cases peristalsis failed to occur.View full abstractDownload PDF (325K) -
Matsuro Fukushima, Yoshinobu Ishikawa, Takahisa Horimai, Minoru Adachi1966 Volume 89 Issue 3 Pages 251-264
Published: July 25, 1966
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSFor the purpose of evaluating the clinical value of ‘in vitro’ sensitivity test of cancer cells to anticancer drugs and the clinical significance of uric acid concentration in the serum and urine after cancer chemotherapy, some experiments were carried out on the DiPaolo's cell agar plate method, and the uric acid level in the serum and urine after the administration of anticancer drugs were examined.
In cases of experimental ascites and solid tumor, DiPaolo's method gave nearly satisfactory results, but in the tests with human cancer cells the method was proved to be available only in 40 per cent of the cases. The uric acid level in the serum of rats bearing AH 130 tumor rose after the administration of anticancer drugs. However, the level did not rise in patients with stomach cancer given with 0.2mg Mitomycin per kg; even in the cases where cancer cells had been proved to be sensitive to the agents by DiPaolo's method, an elevation of the level did not take place after cancer chemotherapy, except in one case treated with Ititromin.View full abstractDownload PDF (1162K) -
Norio Murai1966 Volume 89 Issue 3 Pages 265-272
Published: July 25, 1966
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe present study was designed to investigate the effects of four centrally acting drugs (chlorpromazine 6.0mg/kg, reserpine 0.1mg/kg, meprobamate 60.0mg/kg and phenobarbital 8.0mg/kg) upon the behavioral development, especially development of intelligence, of the offspring of rats, to which these drugs were administered during four days of the early and late periods, from the 5th to 8th and from the 17th to 20th days, of pregnancy.
When the offsprings were 90 days old, the level of their general activity was measured by the revolving drum technique. In both the administration periods the activity level was significantly lower only in the meprobamate -treated group than that in the control group.
Intelligence of 120-day-old animals was tested by Hebb-Williams' enclosed field test. When the administration was done in the early period of gestation, the intelligence of the chlorpromazine-, meprobamate- or phenobarbital-treated groups was each significantly lower than that of the control group. But when the administration was done in the late period of pregnancy, only the meprobamatetreated group showed a significantly lower intelligence than the control group.
Theyse findings were also supported by the results of the brain activity test by a Woodbury's electroshock apparatus.
From these results it is suggested that some of the centrally acting drugs have possibility of causing a retardation of mental development of offspring, and that the prenatal medication in the early period of gestation has a profounder effect than that in the late period.View full abstractDownload PDF (477K) -
Hiyoshimaru Oyamada1966 Volume 89 Issue 3 Pages 273-278
Published: July 25, 1966
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSIt is well known that I'll-triiodothyronine resin sponge uptake test (Triosorb test) is a very useful and easy procedure for the determination of thyroid activity. However, this method has an inevitable problem of fluctuating standard values (resin sponge uptake % of standard serum) of each kit, which makes universal comparison of Triosorb values obtained on patients impossible. The method presented here utilizes 30% correction on the standard value of each kit and provides a solution on the problem due to the fluctuating standard values. Therefore, universal comparison of Triosorb values obtained by different kits having different standard values becomes possible.View full abstractDownload PDF (275K) -
Seiju Onodera1966 Volume 89 Issue 3 Pages 279-291
Published: July 25, 1966
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSBy immunoelectrophoresis at least three immunoglobulins, IgG, IgA and IgM can be recognized but quantitative determination of each immunoglobulin has been hampered by methodological difficulties. Recently, however, Fahey and McKelvey described in details an antibody-agar plate method for this purpose. While the author was following their method, it was found that less antiserum was required by spreading gntiserum on the agar plate than by mixing it with the agar at 56°C. The mixing method required two or three times the amount of antiserum needed for the spreading method.
By the antibody-agar plate method normal serum IgG, IgA and IgM value were calculated as 18.5±3.9mg/ml, 3.6±0.68mg/ml and 1.3±0.37mg/ml, respectively. These normal values for IgG and for IgA are higher than those reported by Fahey and McKelvey.
The serum IgG, IgA and IgM concentration in various diseases were examined and compared with the total protein concentration. The immunoglobulin levels were not necessarily related to the total protein levels. There was characteristic increase of IgG with decrease of IgA and IgM in G multiple myeloma. All immunoglobulins tended to be decreased in cases under long-term steroid treatment. As for immunoglobulin concentration in other conditions, further studies seem to be required to conclude anything definite.View full abstractDownload PDF (3447K) -
Seishi Kawabata, Mizuho Asakawa1966 Volume 89 Issue 3 Pages 293-305
Published: July 25, 1966
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSReticuloendothelial cells (RES cells) of human bone marrow were enzymehistochemically and cytochemically studied. Acid phosphatase staining (azo-dye method) was most suitable for the demonstration of RES cells. Attempts were made to classify RES cells of smear specimens into 5 types according to the size of nucleus, volume of cytoplasm and phagocytosis with the aid of acid phosphatase staining, and ‘RES cell picture;’ was made by calculating the percentage of all types concerned. Analysis of 'RES cell picture' showed the presence of a close correlation between function and morphology of RES cells. Intracytoplasmic bodies of RES cells other than mitochondria, endoplasmic reticulum, Golgi apparatus and ribosome were electron-microscopically studied. Classification of these bodies was made into 5 types, of which relation to acid phosphatase staining was discussed and lysosomal nature of these bodies was mentioned.View full abstractDownload PDF (3131K)
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