All issues
Volume 168 (1992)
- Issue 4 Pages 539-
- Issue 3 Pages 449-
- Issue 2 Pages 67-
- Issue 1 Pages 1-
Volume 168, Issue 4
Displaying 1-9 of 9 articles from this issue
- |<
- <
- 1
- >
- >|
-
IWAO SASAKI, KOUHEI FUKUSHIMA, HIROO NAITO, SEIKI MATSUNO, TUNEO SHIRA ...1992 Volume 168 Issue 4 Pages 539-548
Published: 1992
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSASAKI, I., FUKUSHIMA, K., NAITO, H., MATSUNO, S., SHIRATORI, T. and MAKI, T. Long-Term Results of Pylorus-Preserving Gastrectomy for Gastric Ulcer. Tohoku J. Exp. Med., 1992, 168 (4), 539-548-The postoperative results of pylorus-preserving gastrectomy (PPG) for gastric ulcer performed in 134 patients during the past 25 years (mean postoperative period, 16.6 years) were studied. The incidence of postoperative complications was low. Dumping syndrome occurred in only 4.4% and 0% of cases as assessed by questionnaire and interview, respectively. Four (5.4%) of 74 patients available for this study had ulcer recurrence. In one of these four patients concurrent gastroduodenal ulcer was suspected from preoperative gastric analysis. The site of recurrence was found in all cases to be the remnant antral gland area along the greater curvature between the proper gastric gland area and the duodenum. Basal and maximal acid outputs at the time of relapse were significantly higher in patients with recurrence than in patients without recurrence. The fasting and postprandial serum gastrin levels were high in one patient with recurrence, whose antrum was preserved as long as 3cm proximal to the pyloric ring; this was longer than that described in our original method of PPG. In two other recurrent cases the serum gastrin levels were not different from those in norecurrent cases. Immunohistochemical examination of the residual antrum showed no increase in the G-cell density in patients either with or without recurrence. These results suggest that the long-term quality of life of patients treated with PPG remains favorable. Recurrence rate can be further reduced if PPG is strictly indicated for gastric ulcer only and carried out by meticulous surgical techniques. In the pathogenesis of the ulcer recurrence the role of gastrin release from the residual antral mucosa seems to be limited.- pylorus-preserving gastrectomy; long-term follow-up; postoperative ulcer recurrenceView full abstractDownload PDF (1466K) -
TOSHIYUKI TSURUTA, SADAHIKO MASUKO, HIDEO WATANABE1992 Volume 168 Issue 4 Pages 549-560
Published: 1992
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSTSURUTA, T., MASUKO, S. and WATANABE, H. Immunohistochemical Study of the Sympathetic and Sensory Innervation to the Blood Vessels of the Dog Forepaw. Tohoku J. Exp. Med., 1992, 168 (4), 549-560-Immunohistochemical staining of arteries supplying the dog forepaw showed a dense distribution of nerve fibers which were immunoreactive to tyrosine hydroxylase (TH), neuropeptide Y (NPY), vasoactive intestinal peptide (VIP), substance P (SP), and calcitonin gene-related peptide (CGRP) around the vascular walls. The density of each immunoreactive fiber tended to increase in the peripheral branch of the vascular tree. Retrograde axonal tracing with Fast Blue from the artery revealed that these immunoreactive fibers originated from NPY-containing catecholaminergic as well as VIP/SP/ CGRP-containing non-catecholaminergic neurons in the stellate ganglion and SP/ CGRP-containing neurons in the dorsal root ganglia of segments C7 to Th1. After stellate ganglionectomy, TH-, NPY-, and, VIP-immunoreactive fibers disappeared completely from the arterial walls while approximately 40% of SP- and CGRP- immunoreactive fibers remained. The present results indicate that the artery of the dog forepaw receive triple innervation of adrenergic sympathetic, non- adrenergic sympathetic, and sensory fibers, and suggest that about 40% of SP- and CGRP-immunoreactive fibers are of sensory origin.-perivascular innervation; digital artery; dog forepaw; immunohistochemistryView full abstractDownload PDF (2661K) -
HIROKAZU SATO1992 Volume 168 Issue 4 Pages 561-572
Published: 1992
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSATO, H. Purification of a New Anticoagulant Protein, Calphobindin III, from Human Placenta. Tohoku J. Exp. Med., 1992, 168 (4), 561-572-The Ca2+-phospholipid binding proteins in human placental tissue were investigated with the binding of a placental EDTA extract to liposomes composed of placental phospholipids. A new Ca2+-dependent phospholipid-binding protein different from calphobindin I (CPB I) and calphobindin II (CPB II) was isolated from the EDTA extract, and the purification procedure of this protein was established. The yield of the purified protein was about 1.2mg from one placenta. The protein prolonged the clotting time of normal plasma when coagulation was induced by tissue factor and ellagic acid. This protein had an apparent molecular weight of 32, 000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions, and its isoelectric point was 5.8. Because of its ability to bind phospholipids in the presence of Ca2+, this protein was designated as calphobindin III (CPB III).-Ca2+-dependent phospholipid-binding protein; calphobindin; annexin; anticoagulant; purificationView full abstractDownload PDF (1367K) -
YONGJIAN XU, KOICHIRO SHIRAISHI, MASUKO MORI, MASAKICHI MOTOMIYA1992 Volume 168 Issue 4 Pages 573-582
Published: 1992
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSXU, Y., SHIRAISHI, K., MORI, M. and MOTOMIYA, M. Changes of Fibronectin in the Right and Left Ventricles of Rats Exposed to Chronic Normobaric Hypoxia. Tohoku J. Exp. Med., 1992, 168 (4), 573-582-Changes of fibronectin (FN) in the right and left ventricles of adult rats exposed to chronic normobaric hypoxia were observed by a peroxidase immunohistochemical stain technique and analyzed quantitatively by a point counting method. Fifty-six rats were randomly divided into control groups of day 0 (immediately prior to the experiment), day 5, day 15, and day 30 and hypoxia groups of day 5, day 15, and day 30. Rats of the hypoxia groups were put into a normobaric hypoxia chamber with oxygen concentration adjusted to 10 percent. The rats of the control groups breathed room air. From day 5 on, the ratio of the weight of the right ventricle (RV) to that of the left ventricle (LV) plus interventricular septum (SP), RV/(LV+SP), and the ratio of the weight of the right ventricle (RV) to the body weight (BW), RV/BW, in the hypoxia groups increased significantly as compared with those of the control groups. The amount of immunoreactive FN in the right ventricle increased significantly in the hypoxia groups after exposure to hypoxia environment for 15 days (10.31%±2.15%, mean±S.D.) and for 30 days (9.55%±1.65%) as compared with those in the day 0 control group (3.05%±1.15%, p<0.01), the day 15 control group (3.26%±0.83%, p<0.01), and the day 30 control group (3.19%±0.51%, p<0.01). However, there were no significant changes in the amount of immunoreactive FN in the left ventricle of the hypoxia groups as compared with the control groups. These results suggest that chronic hypoxia may lead to an increase of FN in the hypertrophied right ventricle but not in the left ventricle, which indicates that pulmonary hypertension induced by chronic hypoxia rather than chronic hypoxia itself is a major cause for the increase of FN in the myocardium. The increased FN in the right ventricle may accelerate the accumulation of collagen and, in turn, contribute to the increase of the myocardial stiffness and eventually to the diastolic dysfunction of the hypertrophied right ventricle induced by chronic hypoxia.-fibronectin (FN); hypoxia; right ventricular hypertrophy; pulmonary heart diseaseView full abstractDownload PDF (4111K) -
PANAYIOTIS M. ZAVOS, GRACE M. CENTOLA1992 Volume 168 Issue 4 Pages 583-590
Published: 1992
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSZAVOS, P.M. and CENTOLA, G.M. Methods of Semen Preparation for Intrauterine Insemination and Subsequent Pregnancy Rates. Tohoku J. Exp. Med., 1992, 168 (4), 583-590-Semen for insemination, either intrauterine or in vitro, must be prepared to remove seminal plasma products and/or select the healthier population of sperm prior to use. Traditionally, a double wash technique is performed, with or without subsequent swim-up to isolate the motile fraction if necessary. More recently, the use of the SpermPrep filtration method has gained acceptance, with the benefits of removal of leukocytes and seminal debris from the specimen as well as enhancement of overall sperm quality. In the current study we compared the traditional double wash method without the swim-up to SpermPrep filtration. Intrauterine inseminations (IUI's) were performed in 307 cycles on 148 infertile couples at two different infertility centers in the USA. After complete diagnostic evaluation the couples were offered IUI before proceeding to any other form of assisted reproductive technologies. Semen samples were prepared in human tubal fluid media supplemented with 5% human serum albumin (HSA; location 1) or in Ham's F-10 media supplemented with 3% HSA (location 2), either with the SpermPrep filtration method (ZBL, Inc., Lexington, KY 40523, USA) or the double sperm wash (SW) procedure. Similar sperm numbers were used for the IUI procedure in both treatment groups and locations. The SpermPrep method resulted in significantly higher pregnancy rates (PR) than the SW procedure, independent of location. The clinical pregnancy rates per cycle were statistically lower (p<0.05) in the SW group (20-22% vs. 9-10%). Of significant clinical importance, almost twice as many cycles were required in the SW group to achieve these pregnancies when compared to the SpermPrep group of patients. The results point out that IUI has a significant role to play in the treatment of many infertile couples. Furthermore, the results point out very clearly that selecting high quality spermatozoa via the SpermPrep method resulted in even higher conception rates than the SW method when IUI was applied. Similar improvements in PR using the SpermPrep method may be realized with other artificial reproductive techniques. SpermPrep; spermatozoa; sperm wash; semen fitration; IUI; pregnancy ratesView full abstractDownload PDF (932K) -
JIRO WATARAI, TOSHIO KATO, MITSURU KOBAYASHI, YASUO SEINO, MICHIHIKO K ...1992 Volume 168 Issue 4 Pages 591-598
Published: 1992
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSWATARAI, J., KATO, T., KOBAYASHI, M., SEINO, Y., KITAMURA, M. and ABO, S. Locoregional Control for Esophageal Carcinoma Treated with Irradiation Following Surgery. Tohoku J. Exp. Med., 1992, 168 (4), 591-598-Locoregional failure was analyzed in a total of 34 esophageal carcinoma patients treated with postoperative prophylactic irradiation following curative surgery. All patients had squamous cell carcinoma and no prior treatment. Twelve patients had subsequent lymph node metastasis in the follow-up period. In the 12 patients with node metastasis, there were 5 instances of supraclavicular node metastasis, 7 instances of thoracic inlet node (uppermost part of mediastinum) metastasis, and 3 instances of mediastinal node metastasis. Three patients had 2 metastatic nodes and 9 patients had 1 metastatic node. Intervals between surgery and recurrence were a median of 12 months for mediastinal nodes, 19 months for thoracic inlet nodes, and 26 months for supraclavicular nodes. Ten (52.6%) of the 19 patients treated by using a 12-MeV electron beam had metastatic involvement at the supraclavicular and thoracic inlet nodes. On the other hand, 2 (13.3%) of the 15 patients had the above-described lymph node metastases when treated by using 15-MeV electron, 18-MeV electron, or 10-MV photon beams. The difference in the metastatic rate between these two groups was statistically significant at the level of p<0.05 (chi-square test); this seems to be attributable mainly to the dose level at deep region. Doses of over 50Gy to the thoracic inlet and supraclavicular nodes at deep location were necessary to reduce metastasis-esophageal carcinoma; postoperative irradiation; lymph node metastasisView full abstractDownload PDF (802K) -
YOSHIYUKI OHASHI, MASAYOSHI MINEGISHI, SHIGERU TSUCHIYA, TASUKE KONNO1992 Volume 168 Issue 4 Pages 599-610
Published: 1992
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSOHASHI, Y., MINEGISHI, M., TSUCHIYA, S. and KONNO, T. Three Monoclonal Antibodies against Human LEA-1 α and β Chains with Different Biological Activities. Tohoku J. Exp. Med., 1992, 168 (4), 599-610-Three murine monoclonal antibodies (MAbs) directed against human lymphocyte function-associated antigen-1 (LFA-1, CD11a/CD18), designated as MAY.017, MAY. 035, and MAY. 044, were newly generated. The hybridomas were screened for their ability to inhibit a phorbol ester-stimulated aggregation of Epstein-Barr virus-transformed B-lymphoblastoid cell line (B-LCL). The MAbs bound to peripheral blood leukocytes, T cell lines, B cell lines, and some of myeloid/monocytic lines, but not to B-LCL derived from a patient with leukocyte adhesion deficiency. MAY.035 immunoprecipitated a complex of proteins with molecular masses of 155kDa and 95kDa, while MAY.017 and MAY.044 did a complex of 130kDa, 155kDa and 95 kDa proteins. MAY.035 was shown as to recognize the α chain of LFA-1(CD11a), and both MAY.017 and MAY.044 the β chain of the β2 integrin family (CD18). All the three MAbs inhibited lymphocyte proliferative responses to mitogens or alloantigens. MAY.017 blocked cytolytic activity mediated by natural killer cells. -monoclonal antibody; integrins; lymphocyte function-associated antigen-1; leukocyte; bone marrow transplantationView full abstractDownload PDF (1721K) -
SHINICHIRO KOYAMA, AKIKO OHTSUKA, TAKASHI HORIE1992 Volume 168 Issue 4 Pages 611-619
Published: 1992
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKOYAMA, S., OHTSUKA, A. and HORIE, T. Eucapnic Hyperventilation-Induced Bronchoconstriction in Rabbits. Tohoku J. Exp. Med., 1992, 168 (4), 611-619- We examined whether eucapnic hyperventilation with dry air produces the bronchoconstriction in anesthetized, non-sensitized rabbits and in ovalbumin sensitized rabbits. Eucapnic hyperventilation challenge with dry air containing 5% CO2 at room temperature was performed with 4 non-sensitized and 7 sensitized rabbits by mechanical ventilation for 15min (120 breaths/min, 7ml tidal volume/kg body weight). Total lung resistance (RL) and dynamic compliance (Cdyn) were measured before and 0, 5, 15, and 30min after hyperventilation. In non-sensitized rabbits, RL and Cdyn did not change significantly. However, in sensitized rabbits, RL increased maximally by 48.9%±9.0% at 5min, and then decreased to the baseline level at 30min after challenge. Cdyn decreased maximally by 12.5%± 3.5% at 15min after challenge. These changes were significantly different from the baselines (p<0.05). Furthermore, to investigate the role of histamine on hyperventilation-induced bronchoconstriction (HIB) in sensitized rabbits, we performed the hyperventilation challenges in 5 sensitized rabbits with the pretreatment of H1-receptor antagonist (chlorpheniramine, 1mg/kg, i.v.) and found that the maximum increment of RL was suppressed to 24.2%±7.4% of the control, which was significantly lower than the maximal RL in nontreated sensitized rabbits (p<0.05). We concluded that HIB occurrs only in sensitized rabbits and that histamine may play an important role in the development of HIB in sensitized rabbits.-hyperventilation-induced bronchoconstriction; ovalbumin sensitized rabbits; chlorpheniramineView full abstractDownload PDF (906K) -
OSAMU NISHIZAWA, TOSHIYUKI KAWAHARA, NAOTAKE SHIMODA, KAZUMASA SUZUKI, ...1992 Volume 168 Issue 4 Pages 621-622
Published: 1992
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSNISHIZAWA, O., KAWAHARA, T., SHIMODA, N., SUZUKI, K., FUJIEDA, N., KUDO, T., SUZUKI, T., NOTO, H., HARADA, T. and TSUCHIDA, S. Effect of Methylene Blue on the Vesicourethral Function in the Rats. Tohoku J. Exp. Med., 1992, 168 (4), 621-622-The bladder and urethral activities during the rhythmic bladder contractions were evaluated before and after the intraarterial administration of methlene blue, which prevents the activation of soluble guanylate cyclase. The methlene blue produced an increase in the bladder activity and a decrease in the urethral smooth muscle relaxant response induced with bladder contraction. The L-arginine/nitric oxide pathway seems to modulate the vesicourethral function. -bladder; urethra; nitric oxideView full abstractDownload PDF (186K)
- |<
- <
- 1
- >
- >|