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Volume 131 (1980)
- Issue 4 Pages 313-
- Issue 3 Pages 209-
- Issue 2 Pages 103-
- Issue 1 Pages 1-
Volume 131, Issue 3
Displaying 1-13 of 13 articles from this issue
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UDP-N-Acetylglucosamine 2'-Epimerase of Rat Hepatoma and Its Comparison with the Enzyme of Rat LiverKUNIMI KIKUCHI, SHIGERU TSUIKI1980 Volume 131 Issue 3 Pages 209-214
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSUDP-N-acetylglucosamine 2'-epimerase was partially purified from Yoshida sarcoma, a strain of rat ascites hepatoma, by (NH4)2SO4 precipitation followed by TEAE-cellulose chromatography. The purified enzyme was inhibited by CMP-N-acetylneuraminic acid to similar extent to partially purified rat liver 2'-epimerase. Although the two enzymes were also similar in Km for UDP-N-acetylglucosamine, they were differentiated by chromatography on TEAE-cellulose, DEAE-cellulose, DEAE-Sephadex and hydroxyapatite, tumor enzyme being less tightly bound to these columns than liver enzyme. Similar chromatographic difference was also noted between 2'-epimerases of mouse liver and mouse Ehrlich ascites carcinoma.View full abstractDownload PDF (385K) -
MICHIO HONGO, AKIRA ISHIMORI, AKIO NAGASAKI, TSUNEAKI SATO1980 Volume 131 Issue 3 Pages 215-219
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe role of the duodenum in the regulation of the lower esophageal sphincter pressure was studied in 3 anesthetized dogs with a duodenal fistula by relating the lower esophageal sphincter pressure with plasma gastrin and secretin level during intraduodenal acid infusion test. Instillation of hydrochloric acid into the duodenum produced an initial fall followed by a significant and sustained increase of the lower esophageal sphincter pressure. The initial fall of the lower esophageal sphincter pressure was found to correspond to a prompt but transient rise of plasma secretin concentration, while plasma gastrin did not change significantly throughout the experimental period. It is concluded that duodenum may be an important regulator of the lower esophageal sphincter pressure in the dog through secretin and other unknown factors.View full abstractDownload PDF (288K) -
NORIYUKI TATSUMI, YUKO WADA, TETSUO FUKUDA1980 Volume 131 Issue 3 Pages 221-225
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSBy the addition of phalloidin, an oligopeptide of Amanita phalloides, to platelet-rich-plasma, an increase of its transparency could be observed. The rate of increase of transparency was slow but the maximal intensity corresponded to that of ADP aggregation. Morphologically, many microfilaments were formed in the cytoplasm. But the figures were different from those in platelets in ADP aggregation.View full abstractDownload PDF (1211K) -
ATSUSHI KIMURA, SHIRO HAYASHI, KOICHI TSURUMI1980 Volume 131 Issue 3 Pages 227-239
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSGlycosaminoglycan isolated from the urine of a patient with the Hunter syndrome was composed of heparan sulfate (59.9%), dermatan sulfate (30.6%) and chondroitin sulfate (9.5%), and was heterogeneous in molecular weight (1, 500-10, 000) and in sulfate content (0.35-2.05 moles/mole of hexosamine). About 60% of dermatan sulfate and 10% of heparan sulfate had molecular weight of 7, 000 to 10, 000, while about 10% of the former and 60% of the latter had those of 1, 500 to 3, 500. Sulfate contents of dermatan sulfate and heparan sulfate were inversely related to their molecular weights. Higher total and N-sulfate contents were measured in smaller molecularweight heparan sulfate, and higher acetyl content was in larger molecular-weight heparan sulfate. On the basis of the chemical properties of dermatan sulfate and heparan sulfate isolated in this experiment, their catabolic processes in the Hunter syndrome were discussed.View full abstractDownload PDF (790K) -
ATSUSHI KIMURA, SHIRO HAYASHI, KOICHI TSURUMI1980 Volume 131 Issue 3 Pages 241-247
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe chemical structure of dermatan sulfate (DS) in the urine of a patient with the Hunter syndrome was studied through the analysis of disaccharide units which were derived from the urinary DS by digestion with chondroitinase ABC and separated on a Dowex 1 column. The DS was basically composed of repeating disaccharide units of iduronyl N-acetylgalactosamine 4-sulfate. About 90% of the excess sulfate were linked to the iduronate residues as an additional sulfate group in the unit. N-Acetylgalactosamine 6-sulfate and N-acetylgalactosamine 4, 6-disulfate residues were minor components. No non-sulfated disaccharide unit was detected in the digestion products. Only sulfoiduronate residue was found as the non-reducing terminal sugar of the DS molecule, consistent with the lack of iduronosulfate sulfatase in this disease.View full abstractDownload PDF (457K) -
HIROSHI KANEDA, KENSEI TOBINAI, JUN MATSUMOTO, TAKAKICHI MAETA, TOYOAK ...1980 Volume 131 Issue 3 Pages 249-255
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe effects of vitamin D2 (VD2) on hypocalcemia were studied in 54 hypocalcemic patients (29 males and 25 females) on chronic hemodialysis. Calcium lactate (3g/day) or VD2 (10, 000 IU/day, 50, 000 IU/day and 80, 000 IU/day) were administered for 4 months in order to correct the hypocalcemia. Serum calcium, phosphate and alkaline phosphatase levels were measured and the effects of VD2 on these parameters of calcium metabolism were followed. 1) Calcium lactate or 10, 000 IU/day of VD2 were not effective for the correction of hypocalcemia, while 50, 000-80, 000 IU/day of VD2 were effective. The effects of VD2 on serum calcium concentrations were dose-dependent, and the normalization of serum calcium concentrations was achieved more rapidly with higher doses of VD2. However, in the group treated with 80, 000 IU/day of VD2, many patients developed hypercalcemia, but in the group treated with 50, 000 IU/day of VD2, only a few patients did it. From these results, suitable dose (initial and maintenance doses) of VD2 in dialysed patients would be 50, 000 IU/day. 2) When the responder group (normal serum calcium levels after 4 months of treatment with 50, 000 IU/ day of VD2) and the non-responder group serum calcium levels lower than 4.2 mEq/liter on the same condition) were compared, the durations of dialysis were significantly shorter in the former than those in the latter. This fact may suggest that the effects of VD2 administration on hypocalcemia in dialysed patients are partly dependent on the residual renal function concerning the conversion of 25-OH-D3 into 1, 25 (OH)2D3.View full abstractDownload PDF (465K) -
NOBUO HIWATASHI, TAKAO KIKUCHI, OSAMU MASAMUNE, EIETSU OUCHI, HIKARU W ...1980 Volume 131 Issue 3 Pages 257-260
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSAn investigation was made of the differences between colorectal cancer and adenoma using HLA markers. Subjects included 60 cases of colorectal cancer, 43 of colorectal adenoma and 120 healthy Japanese controls. In comparison with the controls, Bw40 was increased (p<0.02) and B5 was decreased (p<0.005) in the colorectal adenoma cases, whereas in the colorectal cancer cases, Bw35 was increased (p<0.005). B5 was significantly less frequent in the adenoma cases than in the cancer cases (p<0.05). It is suggested that the susceptibility of Bw35 to the causal factors of colorectal cancer is under genetic control.View full abstractDownload PDF (215K) -
TETSURO KATO, RYOSUKE NEMOTO1980 Volume 131 Issue 3 Pages 261-270
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSCell viability under various conditions of cytotoxicity test was assessed by terminal labeling of tumor cells, T24 cell line derived from urinary bladder carcinoma, with 14C-Leucine. Changes of 14C-Leucine incorporation into the cells were fairly proportional to those of viable cell number measured by the trypan blue exclusion method, but a definite correlation between the two measurements was not always found following cytotoxic manipulations. When the cells were labeled immediately after drug treatments, 14C-leucine incorporation usually led to fluctuated and insensitive results presumably due to disturbed metabolic activities unrelated to cell viability and failed to indicate the degree of cell damage. It was shown, however, that the cytotoxicity test was satisfactorily determined by labeling tumor cells with 14C-Leucine after recovery in fresh medium for 24hr. Cytotoxic effects of anticancer drugs with concentration and time-dependency, hyperthermia and phytohemagglutinin-stimulated lymphocytes were demonstrated by the radioactivity incorporated into the target cells on day 2 after removal of the cytotoxic factors. The results indicate that the terminal labeling of tumor cells with 14C-leucine can be used as a rapid and reliable measure sensitivie to cell viability for an in vitro assay system.View full abstractDownload PDF (578K) -
KENJI SASAKI, FUJIO SUZUKI, NAKAO ISHIDA1980 Volume 131 Issue 3 Pages 271-283
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSA mouse model was established for the study of transfer factor (TF). TF was extracted from the spleens of sensitized mice and examined for activity by the footpad test, which showed that mouse and human TF possessed similar properties. Parenteral administration of TF imparted to unsensitized mice immunologically specific, delayed type hypersenstivity within 24hr. The magnitude of the response the recipients showed was proportional to the dose of TF. TF activity was relatively heat-stable, detectable in a fraction of molecular weight of ca 1, 000 and apparently contained nucleic acids. Nude mice responded negatively to TF but following administration of viable naive spleen cells a positive response was observed, which suggests that the thymus plays an important role in the expression of TF phenomenon and that the target of TF is the T cells.View full abstractDownload PDF (802K) -
HAJIME KUDO, TAKASHI WAGA, TATSUSUKE SATO, MASAMICHI OGASAWARA, IZUMI ...1980 Volume 131 Issue 3 Pages 285-288
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSC3H/He mice immunized repeatedly with irradiated (13, 000 rads 60Co) MM46 or MM48, both transplantable ascites mammary carcinomas of the same strain, were subcutaneously challenged with the identical or the different tumor. In mice immunized with irradiated MM46, the growth of challenges of not only MM46 but also MM48 was inhibited. On the other hand, in mice immunized with irradiated MM48, the growth of challenges of MM48 was inhibited, but the inhibition of the growth of MM46 was not observed. Corss-immunity, therefore, was shown by immunization with MM46 but not with MM48. These findings were considered to indicate that MM46 expressed cross-immunity against MM48 because of its high resistance to the irradiation, and that MM48 did not show cross-immunity to MM46 because of its low resistance to the irradiation.View full abstractDownload PDF (273K) -
TAKEO SHIMIZU, MASAHIKO ENDO, ZENSAKU YOSIZAWA1980 Volume 131 Issue 3 Pages 289-299
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSGlycoconjugates and glycogen obtained from the cervix uteri of nonpregnant women of proliferative phase and secretory phase, and from the postpartum cervix uteri were determined by electrophoresis on cellulose acetate membrane and chemical analysis before and after enzymatic digestion and nitrous acid treatment. Although the contents of neutral glycopeptide fraction in these tissues were similar, the glycogen content in the postpartum tissue greatly decreased. The contents in terms of hexosamine of hyaluronic acid, chondroitin sulfate, heparan sulfate and acidic glycopeptides in the postpartum tissue greatly increased in comparison with those in the nonpregnant tissues, whereas that of dermatan sulfate slightly decreased. In addition, dermatan sulfate in the postpartum tissue contained a small portion of non-sulfated disaccharide units, which was not detected in the nonpregnant tissues. The results indicate that significant changes in the contents of glycoconjugates and glycogen of human cervix uteri occur in ripening. The roles of these substances in the cervical ripening are discussed.View full abstractDownload PDF (718K) -
SACHIYOSHI ABE1980 Volume 131 Issue 3 Pages 301-309
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSSelective injections into the left anterior descending (LAD) and left circumflex (LCX) coronary arteries were separately performed in twenty dogs using various hyperosmotic solutions. Ventricular tachycardia (VT) was induced in all dogs studied, by injections of solutions having higher osmolality, whereas solutions with relatively lower osmolality did not produce VT. VT was induced in 100% of trials by injections of 30% saline into the LAD. Injections into the LCX of both 10% and 30% saline also elicited VT in 100%. The VT was produced under classic total cardiac denervation combined with intravenous propranolol (0.6mg/kg) and atropine (0.15mg/kg). Atrioventricular (A-V) block caused by surgical section of the distal part of the His bundle did not effectively block the production of VT. Reliability of this rather simple technique appears to provide us with a new experimental model.View full abstractDownload PDF (681K) -
YUTAKA LMAI, KEISHI ABE, YOICHI OTSUKA, YUTAKA SAKURAI, KAORU YOSHINAG ...1980 Volume 131 Issue 3 Pages 311-312
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSOrally active converting enzyme inhibitor (Captopril, SQ 14225) was administered in a dose of 50mg to 12 normotensive subjects (Group I), 26 essential hypertensive patients (Group II) and 8 renovascular hypertensive patients (Group III). In Group III, 5 of 8 patients had control plasma renin activity (PRA) similar to those in Groups I and II patients, but the PRA response to the administration of Captopril was greater in 7 of 8 patients than that in Groups I and II. These 7 patients had either bilateral or unilateral main renal artery stenosis. Captopril caused no increase in PRA in the remaining 1 who had unilateral renal artery stenosis with contralateral renal aplasia. It is concluded that this provocation test is useful as a screening procedure for the diagnosis of renovascular hypertension.View full abstractDownload PDF (154K)
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