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Volume 88 (1966)
- Issue 4 Pages 305-
- Issue 3 Pages 195-
- Issue 2 Pages 99-
- Issue 1 Pages 1-
Volume 88, Issue 4
Displaying 1-10 of 10 articles from this issue
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Tsuneo Arakawa, Masako Fujii, Haruko Hirono1966 Volume 88 Issue 4 Pages 305-310
Published: April 25, 1966
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSTetrahydrofolate-dependent enzyme activities were investigated upon erythrocyte lysates from two cases with formiminotransferase deficiency syndrome. The activities of both N5, 10-methylene-tetrahydrofolate dehydrogenase and serine-hydroxymethylase were found to be slightly increased. Cyclohydrolase and formate-activating enzyme were found to be normal in their activity.
The results obtained suggested that a metabolic pathway of tetrahydrofolates, including tetrahydrofolate→N5, 10-methylene-tetrahydrofolate→N5, 10-methenyl-tetrahydrofolate→N10-formyltetrahydrofolate, was of an increased activity in erythrocytes in which formiminotransferase was of defective activity.View full abstractDownload PDF (257K) -
Shunshi Irisawa, Masafumi Shirai, Shozaburo Matsushita, Manabu Kagayam ...1966 Volume 88 Issue 4 Pages 311-326
Published: April 25, 1966
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSHistological, histochemical and biochemical investigations were performed on the adenohypophysis, thyroid, adrenal and gonad of diabetic patients and of experimental diabetic animals. The characteristic changes in the adenohypo-physis of diabetics were remarkable increase of alpha cells and distinct decrease of delta cells. In most of the cases, the follicles of the thyroid were large and their epithelial cells were low and flat. The characteristic feature of the adrenal cortex was hyperplasia of the zona fasciculata. The testicular canaliculi were atrophied with thickened basement membranes. Spermatogenesis ceased almost completely in many tubules. The basement membrane of the diabetic testis was stained stronger than the normal with PAS. Spermatogenic cells were stained with Alcian blue; especially the cells near the basement membrane had strong affinity to the dye. A remarkable metachromasia with toluidine blue in the diabetic testicular tissue was recognized at pH 1.5 or 3.0. A significant increase in the values of Qo2 and Qco2 of testicular tissues in diabetics was demonstrated. Almost all diabetics showed decreased urinary excretion of pituitary gonadotropin. The present investigation suggested that these changes were due to deficiency of TSH, ACTH and gonadotropin resulting from the degeneration of the adeno-hypophysis in diabetics. However, the sexual disturbances in diabetics might be due not only to deficient pituitary gonadotropin, but also to degenerative changes of the nerves innervating the gonads.View full abstractDownload PDF (4635K) -
Takeshi Sekine1966 Volume 88 Issue 4 Pages 327-340
Published: April 25, 1966
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe present experiments were carried out to study the effect of acute or sub-acute antrectomy or vagotomy on the sensitivity of the parietal cells to histamine submucosally administered to the fundic gland area of the rabbit stomach. The Congo red-Carbowax method was used for visualizing gastric acid secretion.
The sensitivity of the parietal cells to locally administered histamine was not influenced by acute or subacute vagotomy, but it was substantially reduced by acute or subacute resection of the antrum.
The results of the present study suggest that gastrin released from the antrum, even in subthreshold amounts for eliciting the acid secretion, acts to increase the sensitivity of the parietal cells to histamine.View full abstractDownload PDF (2529K) -
Tsuneo Arakawa, Masako Fujii, Kazuo Ohara, Shuichi Watanabe, Minoru Ka ...1966 Volume 88 Issue 4 Pages 341-352
Published: April 25, 1966
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSIn 1963 a probably new metabolic error of folic acid was reported by us under a term ‘formiminotransferase deficiency syndrome’. It was characterized by mental retardation, hyperfolic acidemia and urinary excretion of large amounts of formiminoglutamic acid (FIGLU) following an oral dose of L-histidine. The primary lesion was demonstrated to be a defective activity of formiminotransferase in liver tissues.
The present paper presented another type of metabolic error of folic acid which consisted of mental retardation, hyperfolic acidemia and the absence of FIGLU-uria following an oral dose of L-histidine. A marked decrease in cyclohydrolase activity was demonstrated in erythrocytes as well as liver tissues biopsied from three infants with this type of metabolic error of folate. A term ‘cyclohydrolase deficiency syndrome’ was suggested for this new entity.
Clinical findings, such as mental retardation, microcephaly and ventricular dilatation in pneumoencephalograms, in cyclohydrolase deficiency syndrome were also observed in formiminotransferase deficiency syndrome.
Hyperfolic acidemia was also a biochemical finding common to both the syndromes.
FIGLU-uria was observed in formiminotransferase deficiency, but absent in cyclohydrolase deficiency.
A definite diagnosis for formiminotransferase deficiency syndrome was made by demonstration of a decreased activity of formiminotransferase in either erythrocytes or liver tissues. In case of cyclohydrolase deficiency a marked decrease in cyclohydrolase activity in either erythrocytes or liver tissues should be demonstrated for a definite diagnosis for this syndrome.View full abstractDownload PDF (528K) -
Noriyoshi Suzuki, Masayuki Toyoda1966 Volume 88 Issue 4 Pages 353-360
Published: April 25, 1966
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThis paper deals with the infrared absorption spectra of bilirubin and calcium bilirubinate in order to provide a better understanding in the analysis of gallstones by means of infrared spectroscopy. The infrared spectra of these two authentic compounds, together with powdered pigmented calcium stones and their fractions, have been measured for the purpose of establishing the position and assignment of the characteristic absorption bands. As a result, several erroneous interpretations by previous authors of the spectrum of bilirubin, especially as regards absorptions of carboxyl groups, have been noted. Moreover, some absorption bands which were previously regarded to be characteristic of calcium bilirubinate have been attributed to ethanol-extractable ingredients of the pigmented calcium stone; calcium bilirubinate, both authentic material and a specimen obtained from the residue from ethanol extraction of the pigmented calcium stone, showed a spectrum suggesting bilirubin to be present in the form of its carboxylate.View full abstractDownload PDF (346K) -
Sennosuke Ohukuzi1966 Volume 88 Issue 4 Pages 361-366
Published: April 25, 1966
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSIn 8 unanesthetized dogs, blood samples from the adrenal vein were collected by a modification of the method of Satake and his co-workers, and analyzed fluorimetrically for adrenaline and noradrenaline. Dogs paced by an attendant on a bicycle were run 2.0-13.1km. Seven out of 8 dogs exhausted completely at the end of running. The adrenaline and noradrenaline secretion rates increas-ed markedly in one dog, in which the body temperature rose after exercise above 42.0°C. The maximal secretion rate was observed in this case at 15min after the end of running, and noradrenaline percentage to total catecholamines released from the adrenal gland at this peak time was 40 per cent. In 5 dogs only a slight increase and in the other 2 no increase in adrenal medullary secretion was observed after exercise.View full abstractDownload PDF (305K) -
Toshiro Sakurada1966 Volume 88 Issue 4 Pages 367-372
Published: April 25, 1966
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSA practical method for separation and quantitative estimation of human thyroid hormones by thin layer chromatography (TLC) is presented. Thin layer chromatography was performed in Kieselguhr G plate washed with 10% HCl. This HCl washing was effective in removing the substances which interfered with the measurement of the iodo amino acid by eerie sulfate-arsenious acid reac- tion. The elution rates of iodo amino acids from thin layer chromatogram were 92% for 3-monoiodo-l-tyrosine (MIT), 93% for 3, 5-diiodo-l-tyrosine (DIT), 90% for 3, 5, 3'-triiodo-l-thyronine (T3) and 92% for l-thyroxine (T4) respectively. The correlation between the quantity of thyroid hormones and optical density was proved to be linear. The extraction rates of each iodo amino acid from human plasma were 75% for MIT, 62% for DIT, 73% for T3 and 63% for T4, and the factors of 1.33 for MIT, 1.61 for DIT, 1.37 for T3 and 1.59 for T4 were calculated. It might be possible to determine the quantity of circulating thyroid hormones by multiplying these factors by the estimated values from the eerie sulfate-arsenious acid color reaction.View full abstractDownload PDF (362K) -
Itaru Ohara, Mitsuo Kijima1966 Volume 88 Issue 4 Pages 373-376
Published: April 25, 1966
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe anticoagulative activity of a newly isolated ω-heparin from whale lung and intestine was compared with that of beef heparin by the intramuscular route in dogs and in two cases of Buerger's disease. The exact comparison could hardly be made, but the authors roughly estimated that the potency of ω-heparin was 1.5-fold that of lente preparation of beef heparin in the same dose level.View full abstractDownload PDF (172K) -
Yoshio Hashimoto, Kisaku Kamiya, Koichi Horio1966 Volume 88 Issue 4 Pages 377-384
Published: April 25, 1966
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe effect of ω-heparin on the blood clotting time was investigated. ω-Heparin was administered to 7 patients by intramuscular injection. The anticoagulative potency of 25mg of ω-heparin was found to be approximately equal to that of 50mg of beef heparin. In laboratory examinations performed in two cases before and after successive injections of ω-heparin, there were no remarkable changes in the blood picture and liver function. Any clinical symptom to be regarded as a side-effect of ω-heparin was not observed.View full abstractDownload PDF (341K) -
Yasuo Tsukahara1966 Volume 88 Issue 4 Pages 385-393
Published: April 25, 1966
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSTo make an international comparison on the mortality, the biennial age-adjusted death rates were calculated for 20 causes in 30 countries, standardized against the total population of 46 countries around 1950. Then the trends in the rates from 1950 to 1961, and the geographical distributions in the period 1960-61 are observed. The common declining tendency of the trends in most countries exists in some causes, such as in tuberculosis and in nephritis and nephrosis. The rates for vascular lesions affecting central nervous system, however, show an increase in some countries, but a decrease in the other. A marked difference between the trends among males and those among females is found in malignant neoplasms, heart disease and bronchitis.View full abstractDownload PDF (524K)
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