The Tohoku Journal of Experimental Medicine
Online ISSN : 1349-3329
Print ISSN : 0040-8727
ISSN-L : 0040-8727
Volume 168 , Issue 3
Showing 1-10 articles out of 10 articles from the selected issue
  • LAURO TOSHIHARU ARAKI, SATOSHI EBIHARA, TAKASHI NAKATSUKA, KIYONORI HA ...
    1992 Volume 168 Issue 3 Pages 449-458
    Published: 1992
    Released: August 31, 2006
    JOURNALS FREE ACCESS
    ARAKI, L.T., EBIHARA, S., NAKATSUKA, T. and HARII, K. Study of Neovascularization of the Jejunal Graft-A New Animal Model. Tohoku J. Exp. Med., 1992, 168 (3), 449-458 - The development of a new rabbit model for study of neovascularization of the jejunal graft is described. The relationship between stripping of the serosal membrane and the speed of the neovascularization process was analyzed. The results demonstrate that the visceral peritoneum acts like a physical barrier, retarding the formation of the new nutrient blood supply from recipient bed to the jejunal graft. - jejunal graft; neovascularization; rabbit; peritoneum
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  • SAYEEDUL ISLAM, ICHIRO OKA, SHINYA FUJITA
    1992 Volume 168 Issue 3 Pages 459-466
    Published: 1992
    Released: August 31, 2006
    JOURNALS FREE ACCESS
    ISLAM, S., OKA, I. and FUJITA, S. Anatomical Study of Preaxial Polydactyly in 158 Hands. Tohoku J. Exp. Med., 1992, 168 (3), 459-466 - Preaxial polydactyly is a common congenital hand anomaly. Here in 138 patients with 158 duplicated thumbs, a new classification of this anomaly was done from analysis of radiographs and operation records. Initially all the duplicated thumbs were arranged into from Type I to VII on the basis of radiological extent of bifurcation. Appendage type was noted as a separate entity. Type IV was found the most common and had 53 thumbs (33.6%) involved. But findings on surgery indicated that the exact level of bifurcation varied in each type, and Type VII became three times as frequent as previously noted from x-ray examination at the time of surgery. Radiologically encountered duplications at IP (Type II), MP (Type IV) and CM (Type VI) joints were specifically classified into five groups from A to E on the basis of the structural changes detected on surgery. Details in each group were discussed. - congenital anomaly; thumb polydactyly; classification
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  • SHIGEAKI NONOYAMA, MASAYUKI NAKAYAMA, JUN ABE, TAKAO KOHSAKA, NOBORU K ...
    1992 Volume 168 Issue 3 Pages 467-474
    Published: 1992
    Released: August 31, 2006
    JOURNALS FREE ACCESS
    NONOYAMA, S., NAKAYAMA, M., ABE, J., KOHSAKA, T., KOBAYASHI, N. and YATA, J. Characterization of Human Thymic Lymphocytes Forming Rosettes with Stromal Cells. Tohoku J. Exp. Med., 1992, 168 (3), 467-474 - The interaction of thymic lymphocytes and stromal cells is believed to be important for T cell development in thymus. In this study, thymic rosettes (TR), which are cell-cell complexes of thymic lymphocytes and stromal cells, were isolated from human thymic tissue, and were characterized. Treating human thymus with collagenase in mild condition, human TR were successfully isolated. Subsequently, TR were purified by the 1G sedimentation method. Human TR consisted of a stromal cell in center surrounded by lymphocytes. The stromal cells were positive for CD14, CD11b, and HLA-DR but negative for thymic epithelial cell specific mAb, UH-1, suggesting that they are macrophage/dendritic cells. The lymphocytes which formed TR (TRL) were mainly double positive (CD4+CD8+) and CD1+ cells, and few of them expressed bright CD3, indicating that TRL are in the intermediate maturation stage. TRL expressed activation markers (Ta1 and HLA-DR) in a significantly higher percentage of cells than did unselected thymocytes. Blocking test revealed that CD11a and CD2 are involved in the binding of TRL and the stromal cells as adhesion molecules. - adhesion molecule; T cell differentiation; human thymic rosettes
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  • SATOSHI KASHIMOTO, AKIHIKO NONAKA, TOSHIHIRO NAKAMURA, TERUO KUMAZAWA
    1992 Volume 168 Issue 3 Pages 475-481
    Published: 1992
    Released: August 31, 2006
    JOURNALS FREE ACCESS
    KASHIMOTO, S., NONAKA, A., NAKAMURA, T. and KUMAZAWA, T. Anesthetic Influences on Myocardial and Hepatic Energy Metabolism in Hemorrhaged Spontaneous Hypertensive Rats. Tohoku J. Exp. Med., 1992, 168 (3), 475-481 - Twenty four spontaneously hypertensive rats (SHRs) were used to assess the influence of anesthetics on myocardial and hepatic energy metabolism after hemorrhage. They were divided into four groups: a control group and three others which received pentobarbital (60mg•Kg-1 ip), 2.2% enflurane, or 1.4% isoflurane. Following a 10-min stabilisation period, blood (2ml•100g body weight-1) was gradually withdrawn over a 5-min period from a femoral artery. Thirty minutes after the induction of hemorrhage, the heart and liver were removed, and myocardial and hepatic metabolites (ATP, lactate, pyruvate, and glycogen) were measured by the enzymatic methods. Metabolic acidosis and decreased hematocrit were noted in all groups after hemorrhage. The mean arterial pressure in rats receiving anesthetics decreased significantly in comparison with the control group. There were significant increases of myocardial and hepatic lactate/pyruvate ratios in rats receiving enflurane when compared with controls. These results suggest that enflurane may be more detrimental than other anesthetics to the maintenance of anesthesia in hypovolemic SHRs. - anesthetics; hemorrhagic shock; metabolism ; spontaneously hypertensive rat
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  • TAKASHIGE MIYAZAKI, SHIGERU KOHNO, KAZUO SASAYAMA, YUICHI INOUE, KOHEI ...
    1992 Volume 168 Issue 3 Pages 483-490
    Published: 1992
    Released: August 31, 2006
    JOURNALS FREE ACCESS
    MIYAZAKI, T., KOHNO, S., SASAYAMA, K., INOUE, Y., HARA, K., OGASAWARA, M., SATO, T. and SUNAMOTO, J. Polysaccharide-Coated Liposomal Amphotericin B for the Treatment of Murine Pulmonary Candidiasis. Tohoku J. Exp. Med., 1992, 168 (3), 483-490 - Amylopectin-coated liposomal amphotericin B was investigated in a murine model of pulmonary candidiasis. The LD50 of amylopectincoated liposomal amphotericin B in normal mice was more than 10.0mg/kg, and that of conventional amphotericin B was 1.2mg/kg. Amylopectin-coated liposomes showed twice the concentration in the lungs of conventional liposomes. Candida albicans was inoculated intratracheally into BALB/C mice. Twenty- four hours later, the number of Candida in the lungs of mice treated with amylopectin-coated liposomes was less than in those treated with conventional liposomes, and amylopectin-coated liposomes improved the survival rate of inoculated mice. Coating liposomes with amylopectin aids the targeting of amphotericin B to the lungs. - liposome; amphotericin B; amylopectin; pulmonary candidiasis; HPLC
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  • RYO KONNO, SHINJI SATO, AKIRA YAJIMA
    1992 Volume 168 Issue 3 Pages 491-497
    Published: 1992
    Released: August 31, 2006
    JOURNALS FREE ACCESS
    KONNO, R., SATO, S. and YAJIMA, A. Detection of Human Papillomavirus DNA in Normal Epithelium and in Squamous Metaplasia of the Uterine Cervix by the Polymerase Chain Reaction. Tohoku J. Exp. Med., 1992, 168 (3), 491-497 - The presence of human papillomavirus (HPV) 16 was studied by using the polymerise chain reaction (PCR) directly on sections histologically defined as normal squamous epithelium and metaplastic squamous tissue of the uterine cervix. Ten specimens of normal epithelium were obtained at hysterectomy from women with uterine leiomyoma. Six specimens of squamous metaplasia were adjacent to the areas of dysplastic epithelium in which HPV 16 DNA had been detected previously by PCR. HPV 16 DNA was amplified specifically and detected in two of 10 normal cervical epithelium specimens and in all of 6 squamous metaplasia specimens adjacent to dysplastic lesions. However, HPV DNA could not be detected in the metaplasia by in situ hybridization. These results suggest that metaplastic squamous tissue adjacent to dysplastic lesions harbors fewer copies of HPV DNA than the dysplastic area and the carcinoma and that the HPV copy number per cell may be relevant to the pathogenesis of cervical carcinoma. - uterine cervix; normal epithelium; squamous metaplasia; human papillomavirus; polymerase chain reaction
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  • EIJI ABE, MICHIO ARAI
    1992 Volume 168 Issue 3 Pages 499-505
    Published: 1992
    Released: August 31, 2006
    JOURNALS FREE ACCESS
    ABE, E. and ARAI, M. Synovial Fluid Ferritin in Traumatic Hemarthrosis, Rheumatoid Arthritis and Osteoarthritis. Tohoku J. Exp. Med., 1992, 168 (3), 499- 505 - Synovial fluid ferritin levels in patients with traumatic hemarthrosis (HA), rheumatoid arthritis (RA), and osteoarthritis (OA) were measured by double antibody radioimmunoassay. Synovial fluid ferritin levels were significantly higher in 60 patients with HA (mean±S.D., 536±536ng/ml) and 39 patients with RA (614±486ng/ml) than in 20 patients with OA (130±119ng/ml) (p<0.01). Individual levels, however, considerably varied. In HA patients, the synovial fluid ferritin level correlated well with the duration of hemarthrosis, but not with hemoglobin, hematocrit, or an inflammatory synovial fluid index such as the leukocyte count. In RA patients, there was no significant correlation between the synovial fluid ferritin levels and any inflammatory parameter, such as catalase activity, synovial leukocyte counts (including polymorphs and monocytes) or the duration of arthritis. Our results indicate that the synovial fluid ferritin level reflects primarily hemoglobin degradation and appears unrelated to inflammation in joint diseases. - synovial fluid; ferritin; hemarthrosis; trauma; rheumatoid arthritis
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  • MASAMI SATO, YASUKI SAITO, NORIYOSHI NAGAMOTO, CHIAKI ENDO, KATUO USUD ...
    1992 Volume 168 Issue 3 Pages 507-513
    Published: 1992
    Released: August 31, 2006
    JOURNALS FREE ACCESS
    SATO, M., SAITO, Y., NAGAMOTO, N., ENDO C., USUDA, K., TAKAHASHI, S., KAN'MA, K., SAGAWA, M., OHTA, S., NAKADA, T. and FUJIMURA, S. An Improved Method of Bronchial Stump Closure for Prevention of Bronchopleural Fistula in Pulmonary Resection. Tohoku J. Exp. Med., 1992, 168 (3), 507-513 - We performed 880 pulmonary resections from January 1982 to June 1988 using Sweet's procedure for closure of the bronchial stump, in which 39 patients (4.4%) developed bronchial fistulas. Bronchoscopic studies showed that bronchopleural fistualas were located mainly at the corner of the stump. This indicates that the corner is the point with the highest tension when Sweet's procedure is employed. In some cases, stumps were injured by suture materials, resulting in bronchopleural fistulas. Since July 1988, bronchial stumps have been closed by using two pairs of teflon pledgets with additional interrupted sutures. From July 1988 to April 1990, 288 patients were treated by this new method, and only one of them developed bronchopleural fistula. This new method prevents injury of the stump by suture material and reduces the tension at the bronchial stump for a long time. Thus, pulmonary resections can be safely employed even after anti-cancer chemotherapy and/or radiation therapy. - bronchopleural fistula; non-absorbable suture; neoadjuvant therapy; teflon pledget
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  • MIKIO IMAMURA, HIDEMI YAMAUCHI
    1992 Volume 168 Issue 3 Pages 515-528
    Published: 1992
    Released: August 31, 2006
    JOURNALS FREE ACCESS
    IMAMURA, M. and YAMAUCHI, H. Effects of Massive Small Bowel Resection on Metabolism of Bile Acids and Vitamin D3 and Gastrin Release in Dogs. Tohoku J. Exp. Med., 1992, 168 (3), - 515-528, The effects of ursodeoxycholic acid (UDCA) and 1α-hydroxyvitamin D3 on pathophysiological changes following massive resection of the distal small bowel (75%) were investigated by using adult beagle dogs. After surgery, body weight decreased, watery diarrhea occurred, and the transit time of the alimentary tract shortened. These undesirable consequences lessened markedly after oral administration of UDCA, though 1α- hydroxyvitamin D3 was not effective. Plasma levels of both 25-hydroxyvitamin D3 and 24, 25-dihydroxyvitamin D3 decreased after surgery, while plasma 1α, 25-dihydroxyvitamin D3 concentrations remained unchanged during the observation period of six months. Although fasting plasma concentrations of total bile acid were not reduced, the integrated response to a meal decreased significantly after surgery in spite of the administration of UDCA. The concentration of UDCA in the gallbladder bile increased markedly in dogs which received UDCA. Taurine-conjugated bile acids accounted for more than 90% of the gallbladder bile. Postprandial hypergastrinemia occurred following the massive small bowel resection in the control group and in the group which received 1α-hydroxyvitamin D3 alone, while it did not occur in the group given UDCA together with 1α- hydroxyvitamin D3. These results indicate that administration of UDCA after massive resection of the small intestine is effective in maintaining good nutritional state. - dog; massive small bowel resection; test meal; ursodeoxycholic acid; 1α-hydroxyvitamin D3; gastrin
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  • TSUGIYASU KANDA, TOMOYUKI YOKOYAMA, TADASHI SUZUKI, KAZUHIKO MURATA
    1992 Volume 168 Issue 3 Pages 529-537
    Published: 1992
    Released: August 31, 2006
    JOURNALS FREE ACCESS
    KANDA, T., YOKOYAMA, T., SUZUKI, T. and MURATA, K. Functional Abnormalities of Circulating Natural Killer Cell Subpopulations in Patients with Dilated Cardiomyopathy. Tohoku J. Exp. Med., 1992, 168 (3), 529-537 We investigated abnormalities in natural killer (NK) cells in the myocardium and circulating blood of 38 patients with idiopathic dilated cardiomyopathy (DCM), 18 patients with hypertrophic cardiomyopathy, 8 patients with primary amyloidosis, and 12 age-matched normal control subjects. Immunohistochemical staining of myocardial biopsies revealed a significantly greater number of CD57-positive NK cells in patients with DCM than that in controls (3.7±2.7 v.s. 1.9±1.6, p<0.05). The New York Heart Association functional class, left ventricular ejection fraction, myocardial fiber diameter, and interstitial fibrosis volume fraction did not differ significantly between the DCM patients who died within five years of diagnosis and the 31 surviving DCM patients. However, there were significantly fewer OD57- positive NK cells in patients who died than in surviving patients (p<0.05). There were no significant differences in the peripheral NK cell activity or the number of NK subset cells between the 16 patients with DCM (n=16) and the 12 age-matched normal controls. In normal controls, the number of some NK cell subpopulations (CD16+, CD57+, CD16+ CD57+, and CD8+ CD57+ cells) were positively correlated with NK cell activity. In patients with DCM, there was no correlation between the number of NK cell Subpopulations and NK cell activity. Our findings indicate that functional abnormalities exist in NK cell subpopulations in patients with DCM, and that these abnormalities may be related to the pathogenesis of DCM. - natural killer cell; immunohistochemistry; NK cell activity; dilated cardiomyopathy
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