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Volume 149 (1986)
- Issue 4 Pages 343-
- Issue 3 Pages 231-
- Issue 2 Pages 107-
- Issue 1 Pages 1-
Volume 149, Issue 3
Displaying 1-13 of 13 articles from this issue
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HIROTAKA OTA, MINEKO FUKUSHIMA, JUNJI, MURATA AKIRA WAKIZAKA, MASAHIR ...1986 Volume 149 Issue 3 Pages 231-240
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSOTA, H., FUKUSHIMA, M., MURATA, J., WAKIZAKA, A. and MAKI, M. Ovarian Membrane Receptors for LH, FSH and Prolactin during the Menstrual Cycle and in Polycystic Ovary Syndrome. Tohoku J. exp. Med., 1986, 149 (3), 231-240-To investigate the role of ovarian membrane receptors for LH, FSH and prolactin of patients with developing Polycystic ovary (PCO) syndrome, seven patients were selected and the receptor function was studied in relation to changes of several serum hormone levels. The results were compared with those from individuals with regular menstrual cycles in the late follicular (LF; n=6) and midluteal (ML; n=6) phases. LH receptor binding in the normal cycle remained low (1.73±0.14fmole/mg homogenate protein) in the LF phase and elevated 3 fold in the ML phase. LH receptors in the PCO patients maintained a higher binding level than that in the LF phase, being close to the ML level. FSH receptors were at a high level in the LF phase (4.09±0.40fmole/mg homogenate protein), but decreased by 23% in the ML phase. In the PCO group the ovarian FSH receptor showed a high level, near to that in the LF phase. Prolactin receptors showed no significant changes among the two controls and PCO group. PCO patients showed increased levels of serum LH and testosterone and a raised ratio of estrone to estradiol, although there was no change in the serum FSH level. These data suggested that the LH receptor binding in PCO was not so low as to the LF level. A lack of the down-regulation mechanism of LH receptors, in spite of the high level of serum LH in PCO, might be one of the clues to elucidate the pathophysiological mechanism in developing PCO syndrome. Elevated levels of the gonadotropin receptors, especially FSH receptors, seem to be involved in the high incidence of ovarian hyperstimulation during hormone treatment.View full abstractDownload PDF (1028K) -
HIROSHI MASUKAWA, MASAYUKI KANEKO, TSUNEO TAKAHASHI, MAKOTO ISHIKAWA, ...1986 Volume 149 Issue 3 Pages 241-249
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSMASUKAWA, H., KANEKO, M., TAKAHASHI, T., ISHIKAWA, M. and SUZUKI, Y. Electrical Measurement of Chloride Secretion in the Perfused Human Jejunum: Effects of Theophylline and Prostaglandin E1. Tohoku J. exp. Med., 1986, 149 (3), 241-249-The effects of intraluminal theophylline or prostaglandin E1 (PGE1) on transepithelial potential difference (PD) were examined in the human jejunum in vivo with a quadruple lumen perfusion tube. To measure transepithelial PDs, we placed one of the Ag/AgCl electrodes in the jejunal lumen and the other in the subcutanous region of the left forearm of the subjects. Infusion of theophylline or PGE1 into the jejunal lumen produced a change in the PD, the lumen being more negatively charged. The magnitude of the change in PD for 2.4×10-3M theophylline was 3.3±0.48mV (mean+S.E., n=6) and that for 10-6M PGE1 was 5.0± 0.60mV (n=6). In addition, the change in the PD induced by simultaneous infusion of both 2.4×10-3M theophylline and 10-7M PGE1 was about twice as large as the sum of those induced by individual infusion of the same concentration of theophylline or PGE1. We concluded that these PD changes occurred primarily by stimulation of electrogenic Cl secretion in the jejunum. Further exploitation of this method to determine PD might be useful for clinical investigation of the intestinal secretion.View full abstractDownload PDF (753K) -
HIROHIKO UKAI, SHIRO TAKADA, SHUNEN INUI, MASAYUKI IKEDA1986 Volume 149 Issue 3 Pages 251-260
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSUKAI, H., TAKADA, S., INUI, S. and IKEDA, M. Relationship between Exposure and Environmental Concentrations in Organic Solvent Workplaces. Tohoku J. exp. Med., 1986, 149 (3), 251-260-The correlation between the exposure concentration (ExpC) of workers and the environmental concentration (EnvC) in their workplaces were examined in 143 printing, painting, gluing, degreasing and other solvent workplaces manned by 535 workers of both sexes. ExpC was measured with carbon felt dosimeters, and EnvC was with grab sampling of air into poly-tetrafluoroethylene bags; both were followed by GC analyses. When ExpC and EnvC were compared on both group and individual basis, the correlation was found to be positive but rather weak, and individual difference in ExpC within the workers of the same workplace was not negligible. Workplaces were classified after regulatory practice into three categories, and attempts were made to find out the conditions so that none among the 174 workers in the 43 Category 1 (i.e., “clean” from regulatory view point) workplaces would be exposed to solvent vapors over the current occupational exposure limit (OEL). Accordingly, it was found that the requirement will be met in case half the OEL is taken as the E value (the EnvC limit set by the regulation). Thus, the same conclusion was reached through the analyses of two separate batchs of experiences, i.e., one in the present study and the other in the previous report (Ikeda and Ohtsuki 1985).View full abstractDownload PDF (941K) -
HARUMI SHISHIDO, ATSUSHI TAKAHASHI, KEIZO MATSUMOTO, AKIKO SAKAI, MASA ...1986 Volume 149 Issue 3 Pages 261-269
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSHISHIDO, H., TAKAHASHI, A., MATSUMOTO, K., SAKAI, A. and ISHIGURO, M. Growth-Enhancing Effect of Branhamella catarrhalis Culture Filtrates on the L-Forms of Haemophilus influenzae. Tohoku J. exp. Med., 1986, 149 (3), 261-269 -Haemophilus influenzae is a common respiratory pathogen and recurrent infection due to H. influenzae is a phenomenon often present in patients with chronic respiratory tract infections. The effects of Neisseria perflava and B. catarrhalis culture filtrates on the growth and reversion of H. influenzae L-forms were determined using the L-forms of H. influenzae induced and subcultured in a medium containing 10% sucrose and these culture filtrates. When each culture filtrate of two strains of N. perflava was added, the viable count of revertants reached a peak of growth after 48hr and exceeded 104CFU/ml even 48 days after the subculture. Adding each culture filtrate of six strains of B. catarrhalis, the viable count of revertants from the L-forms of H. influenzae was much higher than that in the absence of any filtrate (negative control). The filtrate of a culture prepared from one of the six strains of B. catarrhalis was subjected to biochemical analysis. The molecular weight of the growth-enhancing factor(s) was determined to be approximately 1, 000 to 5, 000. Some peptide fractions showed growth-enhancing effects on the L-forms of H. influenzae. These findings suggest that culture filtrates may be important clues as to the correlation between recurrent infections due to H. influenzae and the growth of L-forms in the sputum of the patients with chronic respiratory tract infections, during or after the administration of β-lactam antibiotics.View full abstractDownload PDF (971K) -
HARUMI SHISHIDO1986 Volume 149 Issue 3 Pages 271-282
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSHISHIDO, H. Growth-Enhancing Effects of Culture Filtrates of Sputum Isolates on the L-Forms of Haemophilus influenzae. Tohoku J. exp. Med., 1986, 149 (3), 271-282-The growth-enhancing effects of culture filtrates of respiratory pathogenic bacteria, including Haemophilus influenzae, as well as normal floral bacteria other than Neisseria perflava and Branhamella catarrhalis on L-forms of H. influenzae were examined in vitro, using five species of major respiratory pathogenic bacteria and seven species of normal floral bacteria commonly isolated from the sputum of patients with chronic respiratory tract infections. The growth-enhancing factor(s) was present in the filtrates prepared from the culture of respiratory pathogenic Streptococcus pneumoniae (2) and Staphylococcus aureus (1), the effects of which were as potent as those of a culture filtrate of B. catarrhalis used as the positive control. The culture filtrates of respiratory pathogenic Pseudomonas aeruginosa (2) and Klebsiella pneumoniae (1) had weak growth-enhancing effects on H. influenzae L-forms. The culture filtrates of 21 strains of normal floral bacteria isolated from the sputum including α-hemolytic Streptococci (4), non-hemolytic Streptococci (4), Micrococcus roseus (5), coagulase-negative Staphylococci (3), and Neisseria spp. (5) had growth-enhancing effects on the L-forms of H. influenzae. These data elucidate the significance of L-forms of H. influenzae in recurrent infections due to H. influenzae in patients with chronic respiratory tract infections.View full abstractDownload PDF (1392K) -
TAKASHI MORITA, MARCIA A. WHEELER, IKUO MIYAGAWA, SHUN KONDO, ROBERT M ...1986 Volume 149 Issue 3 Pages 283-285
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSMORITA, T., WHEELER, M.A., MIYAGAWA, I., KONDO, S. and WEISS, R.M. Effects of Forskolin on Contractility and Cyclic AMP Levels in Rabbit Detrusor Muscle. Tohoku J. exp. Med., 1986, 149 (3), 283-285-Forskolin caused a concentration-dependent relaxation and increase in cyclic AMP levels in rabbit detrusor muscle. Propranolol, a β-adrenoceptor antagonist, did not affect the relaxation induced by forskolin. 3-isobutyl-1-metylxanthine (IBMX), a cyclic nucleotide phosphodiesterase inhibitor, potentiated the relaxation induced by forskolin. These data suggest that the relaxation of rabbit detrusor muscle induced by forskolin is mediated by cyclic AMP accumulation resulting from activation of adenylate cyclase.View full abstractDownload PDF (279K) -
FUMI AOKI, HIROSHI NAGASAKI, RYUICHI NAKAMURA1986 Volume 149 Issue 3 Pages 287-291
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSAOKI, F., NAGASAKI, H, and NAKAMURA, R. The Relation of Integrated EMG of the Triceps Brachii to Force in Rapid Elbow Extension. Tohoku J. exp. Med., 1986, 149 (3), 287-291-The relationships between the integrated EMG (iEMG) of the first burst activity of the triceps brachii muscle and biomechanical variables such as acceleration, velocity and kinetic energy were examined for ballistic elbow extension in five healthy men. The duration of the first burst ceased within 100 msec in every subject. Although the regression analysis of each variable to iEMG was all significant, the correlation coefficient was the largest for mean or peak acceleration and the smallest for kinetic energy. The motor center would control the force output to initiate ballistic movement by changing the amount of the EMG activity keeping its duration constant.View full abstractDownload PDF (442K) -
KAORU KOIKE, TAKAMASA OHNUKI, KAZUHIRO OHKUDA, SUMIO NITTA, TASUKU NAK ...1986 Volume 149 Issue 3 Pages 293-305
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKOIKE, K., OHNUKI, T., OHKUDA, K., NITTA, S. and NAKADA, T. Branching Architecture of Canine Pulmonary Arteries: A Quantitative Cast Study. Tohoku J. exp. Med., 1986, 149 (3), 293-305-The purpose of this investigation is to analyze the branching pattern of pulmonary artery. The excised right lower lobes of six mongrel dogs were fixed by perfusion with 1.2% glutaraldehyde solution. Pressure of the pulmonary artery, vein and airway were controlled during perfusion. After fixation, resin was injected manually into the artery. When resinwas completely polymerized, the lung was placed in 15N NaOH solution to remove lung tissue. The diameters and lengths of branches were measured by stereomicroscopy. The results obtained were as follows: (1) The basically dichotomous pulmonary arteries showed not only fairly regular dichotomies but monopolies, in the latter of which the diameter of parent branch was almost the same as that of its larger daughter branch. (2) The diameter of monopodiel branch was less than 500μm, even when the parent branch was a large central segment. In arteries of over 1, 000μm in diameter, 75% or more of branchings were monopodies, whereas in small arteries of 200-300μm in diameter, half the bifurcations were monopolies. (3) At a bifurcation, the average daughter-to-parent diameter ratio was 0.8-0.9 for the larger daughter branch and 0.5 for the smaller one. (4) The average length of branches was in proportion to their average diameter.View full abstractDownload PDF (1039K) -
HIROSHI OHE, HIROKI WATANABE, MASAHITO SAITOH, J. EDSON PONTES, GERALD ...1986 Volume 149 Issue 3 Pages 307-316
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSOHE, H., WATANABE, H., SAITOH, M., PONTES, J.E. and MURPHY, G.P. Evaluation of Effect of Treatment for Primary Lesions of Stage D2 Prostatic Cancer by Means of Transrectal Ultrasonotomography. Tohoku J. exp. Med., 1986, 149 (3), 307-316-The effect of treatment for primary prostatic lesions of Stage D2 prostatic cancer was evaluated by means of transrectal ultrasonotomography and was compared with that for distant metastatic lesions. A new score system was introduced to assess the effect of treatment for the primary lesions of prostatic cancer from the ultrasonic findings of the prostate on a single examination after treatment. In 19 subjects evaluated as effective or moderate in the treatment of primary lesions, 9 were also evaluated as effective or moderate in the treatment of metastatic lesions, while 10 were judged as poor in the treatment of metastatic lesions. In 12 subjects which were evaluated as poor in the treatment of primary lesions, 3 were judged as moderate in the effect of treatment of metastatic lesions, while 9 were judged as poor. It was proved that the correct evaluation of the primary prostatic tumor was required to perform adequate treatment for Stage D2 prostatic cancer.View full abstractDownload PDF (879K) -
TADASHI TERUI, TAIZO KATO, RYUJI SUZUKI, KATSUO KUMAGAI, HACHIRO TAGAM ...1986 Volume 149 Issue 3 Pages 317-322
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSTERUI, T., KATO, T., SUZUKI, R., KUMAGAI, K. and TAGAMI, H. Effect of Interleukin 1 on Chemotaxis and Chemiluminescence of Human Neutrophils. Tohoku J. exp. Med., 1986, 149 (3), 317-322 - We studied the influence of interleukin 1 (IL 1) on chemotaxis and chemiluminescence (CL) of human neutrophils. We found that IL 1 showed chemotactic activity within the concentrations ranging from 1 to 200U/ml, whereas no luminol-or lucigenin-dependent CL was noted in neutrophils with the stimulation of IL 1 at the concentrations ranged from 1 to 100U/ml. Moreover, no enhancement of zymosan-induced CL was observed in neutrophils following prior exposure to IL 1 at a concentration of 100U/ml for 60min at 37°C. We think that IL 1 may be responsible for the inflammatory reaction only through its chemotactic activity for neutrophils without enhancement of the oxidative metabolism of neutrophils.View full abstractDownload PDF (575K) -
TAKASHI KONDO, FUMIHIRO SHIONOZAKI, SHIGEFUMI FUJIMURA, ATSUSHI YAMAUC ...1986 Volume 149 Issue 3 Pages 323-328
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKONDO, T., SHIONOZAKI, F., FUJIMURA, S., YAMAUCHI, A., HANDA, M., OKABE, T., SAITO, R., ICHINOSE, T., SHIRAISHI, Y. and NAKADA, T. A Rat Model for Monitoring Homograft Rejection of the Lung. Tohoku J. exp. Med., 1986, 149 (3), 323-328 - A new surgical procedure was devised for the experimental homograft of the lung. The pulmonary artery and vein and bronchus were anastomosed under the operation microscope using interrupted sutures in group W (30 outbred Wistar rats) and continuous sutures in group F (7 inbred F344/Ducrj Rats). Mean ischemia time during the operation was 95.3±10.3min in the group W and 55.6± 5.8 in group F. Eight out of 30 rats (27%) of group W and all rats of group F survived more than 24hr after the surgery. Pulmonary functions of grafted lungs were evaluated by serial x-rays and the perfusion ratio was 27.4±11.6% (mean± S.D.) in group W and 25.4±7.7% in group F. A patent opening of the hilar anastomosis in these rats was confirmed at autopsy. This model with continuous suture would be useful for the rat lung transplantation.View full abstractDownload PDF (1104K) -
HIROSHI TAKAHASHI, NOBUO TSUDA, FUMIAKI TEZUKA, HARUO OKABE1986 Volume 149 Issue 3 Pages 329-340
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSTAKAHASHI, H., TSUDA, N., TEZUKA, F. and OKABE, H. Immunohistochemical Localization of Carcinoembryonic Antigen in Carcinoma in Pleomorphic Adenoma of Salivary Gland: Use in the Diagnosis of Benign and Malignant Lesions. Tohoku J. exp. Med., 1986, 149 (3) 329-340-The localization of carcinoembryonic antigen (CEA) and lysozyme (LZM) was immunohistochemically studied in 34 carcinomas arising in benign pleomorphic adenomas and 25 normal salivary glands in order to assess its potential diagnostic value. (1) CEA in the normal salivary gland was located in luminal cell membranes of intercalated duct cells and serous acinar cells. (2) Strongly positive cell surface and intraluminal staining of CEA appeared in the areas of gland-forming pattern in pleomorphic adenoma. (3) CEA activity was detected in 7/9 cases (78%) of adenocarcinoma, 10/11 cases (91%) of epidermoid carcinoma, 3/8 cases (38%) of anaplastic carcinoma, 5/5 cases (100%) of mucoepidermoid carcinoma, and 1/1 case (100%) of adenoid cystic carcinoma. CEA was always present in the cytoplasm of epithelial cells and luminal contents of neoplastic glands. CEA in epidermoid carcinoma may occasionally react strongly in the cytoplasm. (4) Lysozyme-immunoreactivity was detected in the cytoplasm of intercalated duct cells and serous acinar cells of the normal salivary gland but little or no LZM was observed in any of the tumors. These results suggest that the presence of CEA could be a useful marker that provides valuable information for the differential diagnosis between benign and malignant areas of carcinoma in pleomorphic adenoma of the salivary gland. Moreover, LZM could be of valuable use for discriminating neoplastic from non-neoplastic tissue of salivary glands.View full abstractDownload PDF (6618K) -
MASACHIKA SENBA, KENJI IRIFUNE, MITSUO KAKU1986 Volume 149 Issue 3 Pages 341-342
Published: 1986
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSENBA, M., IRIFUNE, K. and KAKU, M. Rapid Identification of Klebsiella pneumoniae in the Lung Tissue by Fluorescence Microscopy. Tohoku J. exp. Med., 1986, 149 (3), 341-342 - Klebsiella pneumoniae is stained weekly with hematoxylin and eosin, moderately with periodic acid Schiff (PAS), and strongly by the Warthin-Starry method. Staining by the Warthin-Starry method, however, takes more than one hour. Therefore, we developed a new procedure for rapid identification of K. pneumoniae in paraffin sections of the lung under a fluorescence microscope.View full abstractDownload PDF (273K)
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