Influence of some salts which are contained physiologically in blood, KCl, CaCl
2, NaCl and hydrogen ions, on oxygen consumption of liver tissue of mouse was studied by Warburg's method (1923).
(1) Change of KCl-concentration in Ringer's solution (0.02-0.06%) had little influence on the oxygen consumption both at 30° and 38°. Temperature coefficients Q
10 and μ were therefore not different at different KCl-concentration.
(2) At 30° the oxygen consumption seemed to be slightly accelerated by higher concentration of CaCl
2, but at 38° without any influence; the temperature coefficients Q
10 and μ were therefore slightly smaller at higher concentrations of CaCl
2.
(3) In phosphate-Ringer's solution with various concentrations of NaCl (0.8-1.2%), the oxygen consumption was slightly smaller at higher NaCl-concentration both at 30° and 38°.
In bicarbonate-Ringer's solution (NaCl, 0.3-1.5%) the consumption showed the same tendency as in the former case both at 30° and 38°, but the absolute value of the consumption was distinctly larger. The temperature coefficients Q
10 and μ were generally smaller at higher NaCl-concentration.
(4) The oxygen consumption was influenced in relatively less degree by change of the hydrogen ion concentration in Ringer's solution within the range 7.44-5.50, which was prepared by primary and secondary phosphate mixture. The influence was manifested only at much higher H
+-concentration (at
pH smaller than 6.0), where the oxygen consumption was distinctly less, especially at later experimental period.
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